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In vitro studies of gadolinium-DTPA conjugated with monoclonal antibodies as cancer-specific magnetic resonance imaging contrast agents

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Abstract

The monoclonal antibodies, 9.2.27 against human melanoma cell lines and WM53 against leukemia cell lines, were conjugated with cyclic anhydride gadolinium-diethylenetriaminepenta-acetic acid (Gd-cDTPAa) and used as tumorspecific contrast agents in magnetic resonance imaging (MRI). The data indicate that Gd-DTPA-9.2.27 in solution decreased the T1 relaxation of water protons at 7.0 Tesla (300 MHz) in direct proportion to the gadolinium concentration, and this effect was greater than in Gd-DTPA solutions. These conjugates show high specificity for melanoma and leukemia cell lines. T1 relaxation time at 7.0 Tesla, measured for the melanoma cell line (MM-138) and leukemia cell line (HL-60) after incubation at 37 °C for 4 hr, were significantly decreased (approximately 25%) relative to controls. The gadolinium concentration in cells and washing solutions was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES). A linear relationship was observed between T1 relaxation rates and gadolinium concentrations obtained by ICP-AES. The ICP-AES results showed no gadolinium uptake in the nontargeted HT-29 colorectal cancer cells.

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Authors and Affiliations

  1. Center for Experimental Radiation Oncology, Shahrekord University of Medical Sciences, Iran

    D. Shahbazi-Gahrouei

  2. Centre for Experimental Radiation Oncology, St George Cancer Care Centre, 2217, Kogarah, NSW

    S. M. Rizvi & B. J. Allen

  3. University of Western Sydney, Nepean

    M. A. Williams

Authors
  1. D. Shahbazi-Gahrouei
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  2. S. M. Rizvi
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  3. M. A. Williams
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  4. B. J. Allen
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Correspondence to B. J. Allen.

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Shahbazi-Gahrouei, D., Rizvi, S.M., Williams, M.A. et al. In vitro studies of gadolinium-DTPA conjugated with monoclonal antibodies as cancer-specific magnetic resonance imaging contrast agents. Australas. Phys. Eng. Sci. Med. 25, 31 (2002). https://doi.org/10.1007/BF03178372

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  • DOI: https://doi.org/10.1007/BF03178372

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