Skip to main content
SpringerLink
Log in

In vitro bone formation on coral granules

  • Regular Papers
  • Published:
In Vitro Cellular & Developmental Biology Aims and scope Submit manuscript

Summary

We investigated the ability of fetal rat bone cells isolated after collagenase digestion to differentiate in vitro and to produce a mineralized matrix on coral granules. Scanning electron microscopy examination of the surface of the seeded coral granules revealed that cells attached, spread, and proliferated on the material surface. Bone nodule formation was studied in this in vitro system by direct examination under an inverted phase contrast microscope. The initial event observed was the appearance of cells with phosphatase alkaline activity arranged in several layers and forming a three-dimensional organization around the coral particles. By Day 7, nodule formation began and a refringent material appeared and extended to the background cells during the following days. By Day 15, some coral granules were embedded in a mineralized matrix. Histologic results demonstrated the formation of a mineralized tissue with the appearance of woven bone.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bellows, C. G.; Aubin, J. E.; Heersche, J. N. M., et al. Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif. Tissue Int. 38:143–154; 1986.

    Article  PubMed  CAS  Google Scholar 

  2. Bhargava, U.; Bar-Lev, M.; Bellows, C. G., et al. Ultrastructural analysis of bone nodules formed in vitro by isolated fetal rat calvaria cells. Bone 9:155–163; 1988.

    Article  PubMed  CAS  Google Scholar 

  3. Cheung, H. S.; Story, M. T.; McCarty, D. J. Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells. Arthritis Rheum. 27:668–674; 1984.

    Article  PubMed  CAS  Google Scholar 

  4. Cheung, H. S. In vitro cartilage formation on porous hydroxyapatite ceramic granules. In Vitro Cell. Dev. Biol. 21:353–357; 1985.

    PubMed  CAS  Google Scholar 

  5. Cheung, H. S.; Haak, M. H. Growth of osteoblasts on porous calcium phosphate ceramic: an in vitro model for biocompatibility study. Biomaterials 10:63–67; 1989.

    Article  PubMed  CAS  Google Scholar 

  6. Collin, P.; Nefussi, J. R.; Boy-Lefèvre, M. L., et al. Analysis of the organic matrix synthesized by osteoblastic cells during in vitro mineralization. J. Dent. Res. 65:abstract 1016; 1986.

    Google Scholar 

  7. Collin, P.; Nefussi, J. R.; Boy-Lefèvre, M. L., et al. Expression of bone-associated proteins in mineralized matrix synthesized by rat osteoblastic cells in vitro. Paris: Société Française du Tissu Conjonctif; 1988.

    Google Scholar 

  8. Chiroff, R. T.; White, E. W.; Weber, K. N., et al. Tissue ingrowth of replamineform implants. J. Biomed. Mater. Res. 9:29–45; 1975.

    Article  PubMed  CAS  Google Scholar 

  9. Davies, J. E.; Causton, B.; Bovell, Y., et al. The migration of osteoblasts over substrata of discrete surface charge. Biomaterials 7:231–233; 1986.

    Article  PubMed  CAS  Google Scholar 

  10. Davies, J. E.; Tarrant, S. F.; Matsuda, T. Interaction between primary bone cell cultures and biomaterials. Part 1: Method; the in vitro and in vivo stages. In: Biomaterials and clinical applications. Amsterdam: Elsevier Science Publishers B.V.; 1988.

    Google Scholar 

  11. Ecarot-Charrier, B.; Glorieux, F. H.; van der Rest, M., et al. Osteoblasts isolated from mouse calvaria initiate matrix mineralization in culture. J. Cell Biol. 96:639–643; 1983.

    Article  PubMed  CAS  Google Scholar 

  12. Ernst, M.; Froesch, E. R. Osteoblast-like cells in a serum-free methylcellulose medium form colonies: effects of insulin and insulin-like growth factor I. Calcif. Tissue Int. 40:27–34; 1987.

    Article  PubMed  CAS  Google Scholar 

  13. Gratzner, H. G.; Leif, R. C.; Ingram, D. J., et al. The use of antibody specific for bromodeoxyuridine for the immunofluorescent determination of DNA replication in single cells and chromosomes. Exp. Cell Res. 95:88–94; 1975.

    Article  PubMed  CAS  Google Scholar 

  14. Gregoire, M.; Orly, I.; Kerebel, L. M., et al. In vitro effects of calcium phosphate biomaterials on fibroblastic cell behaviour. Biol. Cell. 59:255–260; 1987.

    PubMed  CAS  Google Scholar 

  15. Guillemin, G.; Patat, J. L.; Fournié, J., et al. The use of coral as a bone graft substitute. J. Biomed. Mater. Res. 21:557–567; 1987.

    Article  PubMed  CAS  Google Scholar 

  16. Guillemin, G.; Meunier, A.; Dallant, P., et al. Comparison of coral resorption and bone apposition with two natural corals of different porosities. J. Biomed. Mater. Res. 23:765–779; 1989.

    Article  PubMed  CAS  Google Scholar 

  17. Harmand, M. F.; Bordenave, L.; Duphil, R., et al. Human differentiated cell cultures: in vitro models for characterization of cell/biomaterial interface. In: Christel, P.; Meunier, A.; Lee, A. J. C., eds. Biological and biomechanical performance of biomaterials. Amsterdam: Elsevier; 1986:361–366.

    Google Scholar 

  18. Holmes, R. E.; Bucholz, R. W.; Mooney, V. Porous hydroxyapatite as a bone graft substitute in diaphyseal defects: a histometric study. J. Orthop. Res. 5:114–121; 1987.

    Article  PubMed  CAS  Google Scholar 

  19. Inoue, T.; Cox, J. E.; Pilliar, R. M., et al. Effect of the surface geometry of smooth and porous-coated titanium alloy on the orientation of fibroblasts in vitro. J. Biomed. Mater. Res. 21:107–126; 1987.

    Article  PubMed  CAS  Google Scholar 

  20. Itakura, Y.; Kosugi, A.; Sudo, H., et al. Development of a new system for evaluating the biocompatibility of implant materials using an osteogenic cell line (MC3T3-E1). J. Biomed. Mater. Res. 22:613–622; 1988.

    Article  PubMed  CAS  Google Scholar 

  21. Jansen, J. A.; de Wijn, J. R.; Wolters-Lutgerhorst, J. M. L., et al. Ultrastructural study of epithelial cell attachment to implant materials. J. Dent. Res. 64:891–896; 1985.

    PubMed  CAS  Google Scholar 

  22. Jones, S. J.; Boyde, A. The migration of osteoblasts. Cell Tissue Res. 184:179–183; 1977.

    Article  PubMed  CAS  Google Scholar 

  23. Jones, S. J.; Boyde, A. Colonization of various natural substrates by osteoblasts in vitro. Scanning Electron Microsc. II:529–538; 1979.

    Google Scholar 

  24. Kang, I. K.; Ito, Y.; Sisido, M., et al. Attachment and growth of fibroblast cells on polypeptide derivatives. J. Biomed. Mater. Res. 23:223–239; 1989.

    Article  PubMed  Google Scholar 

  25. Maroudas, N. G. Chemical and mechanical requirements for fibroblast adhesion. Nature 244:353–354; 1973.

    Article  PubMed  CAS  Google Scholar 

  26. Matsuda, T.; Davies, J. E. The in vitro response of osteoblasts to bioactive glass. Biomaterials 8:275–284; 1987.

    Article  PubMed  CAS  Google Scholar 

  27. Nefussi, J. R.; Boy-Lefèvre, M. L.; Boulekbache, H., et al. Mineralization in vitro of matrix formed by osteoblasts isolated by collagenase digestion. Differentiation 29:160–168; 1985.

    Article  PubMed  CAS  Google Scholar 

  28. Nefussi, J. R.; Septier, D.; Collin, P., et al. A comparative ultrahistochemical study of glycosaminoglycans with cuprolinic blue in bone formed in vivo and in vitro. Calcif. Tissue Int. 44:11–19; 1989.

    Article  PubMed  CAS  Google Scholar 

  29. Nefussi, J. R.; Pouchelet, M.; Collin, P., et al. Microcinematographic and autoradiographic kinetic studies of bone cell differentiation in vitro: matrix formation and mineralization. Bone 10:345–352; 1989.

    Article  PubMed  CAS  Google Scholar 

  30. Nijweide, P. J.; van Iperen-van Gent, A. S.; Kawilarang-de Haas, E. W., et al. Bone formation and calcification by isolated osteoblast-like cells. J. Cell. Biol. 93:318–323; 1982.

    Article  PubMed  CAS  Google Scholar 

  31. Ouhayoun, J. P.; Issahakien, S.; Patat, J. L., et al. Etude de la dynamique de réparation osseuse favorisé par 3 biomatériaux, phosphate tricalcique, hydroxyapatite poreuse et corail naturel, implantés dans la mandibule du porc nain. In: Baquey, C.; Dupuy, B., eds. Hybrid artificial organs. Colloque INSERM 117:345–350; 1989.

  32. Osdoby, P.; Caplan, A. I. First bone formation in the developing chick limb. Dev. Biol. 86:147–156; 1981.

    Article  PubMed  CAS  Google Scholar 

  33. Roux, F. X.; Brasnu, D.; Loty, B., et al. Madreporic coral: a new bone graft substitute for cranial surgery. J. Neurosurg. 69:510–513; 1988.

    Article  PubMed  CAS  Google Scholar 

  34. Shelton, R. M.; Whyte, I. M.; Davies, J. E. Interaction between primary bone cell cultures and biomaterials Part 4: Colonization of charged polymer surfaces. In: Biomaterials and clinical applications. Amsterdam: Elsevier Science Publishers B.V.; 1987.

    Google Scholar 

  35. Tenenbaum, H. C.; Heersche, J. N. M. Differentiation of osteoid-producing cells in vitro: possible evidence for the requirement of a microenvironment. Calcif. Tissue Int. 38:262–267; 1986.

    Article  PubMed  CAS  Google Scholar 

  36. Van der Valk, P.; van Pelt, A. W. J.; Busscher, H. J., et al. Interaction of fibroblasts and polymer surfaces. Relationship between surface free energy and fibroblast spreading. J. Biomed. Mater. Res. 17:807–817; 1983.

    Article  PubMed  Google Scholar 

  37. Wilson, J.; Pigott, G. H.; Schoen, F. J., et al. Toxicology and biocompatibility of bioglasses. J. Biomed. Mater. Res. 15:805–817; 1981.

    Article  PubMed  CAS  Google Scholar 

  38. Wlodarski, K. H.; Reddi, A. H. Alkaline phosphatase as a marker of osteoinductive cells. Calcif. Tissue Int. 39:382–385; 1986.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biologie-Odontologie, Université Paris VII, 2, place Jussieu, F-75251, Paris Cedex 05, France

    J. M. Sautier, J. R. Nefussi & N. Forest

  2. Laboratoire de Biologie du Développement, Université Paris VII, 2, place Jussieu, F-75251, Paris Cedex 05, France

    H. Boulekbache

Authors
  1. J. M. Sautier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. R. Nefussi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Boulekbache
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Forest
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the University of Paris VII and the Dental Association of Garancière. This work is a part of Doctorat d'Université that J. M. Sautier will be submitting to the University of Paris VII.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sautier, J.M., Nefussi, J.R., Boulekbache, H. et al. In vitro bone formation on coral granules. In Vitro Cell Dev Biol 26, 1079–1085 (1990). https://doi.org/10.1007/BF02624444

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02624444

Key words

Search

Navigation