Abstract
A setiform dematiaceous hyphomycete,Kionochaeta spissa was newly collected and isolated from evergreen broad-leaved forests in the southern parts of Japan. Except for its dematiaceous nature, the species is morphologically similar to a nectriaceous hyphomycete,Chaetopsina fulva. The morphology and cultural properties of the Japanese isolates ofK. spissa were described, and the phylogenetic relationships betweenChaetopsina (C. fulva (type species)) andKionochaeta (K. ramifera (type species),K. spissa andK. ivoriensis) were inferred based on nuclear encoded small subunit (18S) rDNA sequences using the neighbor-joining method.Chaetopsina andKionochaeta were found to be phylogenetically related to the Hypocreales and Sordariales, respectively. Both should be maintained as separate genus for phylogenetic classification. The morphological resemblance especially betweenC. fulva andK. spissa is an example of the convergent evolution.
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Ainsworth, G. C., James, P. W. and Hawksworth, D. L. 1971. Ainsworth & Bisby's dictionary of the fungi, 6th ed. Commonwealth Mycological Institute, Kew.
Berbee, M. L. 1996. Loculoascomycete origins and evolution of filamentous ascomycete morphology based on 18S rRNA gene sequence data. Mol. Biol. Evol.13: 462–470.
Castañeda, R. F. 1986. Deuteromycotina de Cuba. Hyphomycetes IV. Inst. Invest. Fund. Agric. Trop. (Alejandro de Humboldt), Habana.
Crane, J. L. and Schoknecht, J. D. 1982. Hyphomycetes from freshwater swamps and hammocks. Can. J. Bot.60: 369–378.
Crous, P. W., Wingfield, M. J. and Kendrick, W. B. 1994.Konochaeta pini sp. nov. andVerrucophragmia splendens gen. nov. from leaf litter in South Africa. Mycologia86: 447–450.
DiCosmo, F., Berch, S. and Kendrick, B. 1983.Cylindrotrichum, Chaetopsis, and two new genera of Hyphomycetes,Kylindria andXenokylindria. Mycologia75: 949–973.
Ellis, M. B. 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew.
Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution39: 783–791.
Gargas, A. and Taylor, J. W. 1995. Phylogeny of discomycetes and early radiations of the apothecial Ascomycotina inferred from SSU rDNA sequence data. Exp. Mycol.19: 7–15.
Haase, G., Sonntag, L., van de Peer, Y., Uijthof, J. M. J., Podbielski, A. and Melzer-Krick, B. 1995. Phylogenetic analysis of ten black yeast species using nuclear small subunit rRNA gene sequences. Antonie van Leeuwenhoek68: 19–33.
Hawksworth, D. L., Kirk, P. M., Sutton, B. C. and Pegler, D. N. 1995. Ainsworth & Bisby's dictionary of the fungi, 8th ed. International Mycological Institute, Egham.
Hawksworth, D. L., Sutton, B. C. and Ainsworth, G. C. 1983. Ainsworth & Bisby's dictionary of the fungi, 7th ed. Commonwealth Mycological Institute, Kew.
Hendriks, L., De Baere, R., van de Peer, Y., Neefs, J., Goris, A. and De Wachter, R. 1991. The evolutionary position of the rhodophytePorhyra umbilicalis and the basidiomyceteLeucosporidium scottii among other eukaryotes as deduced from complete sequences of small ribosomal subunit RNA. J. Mol. Evol.32: 167–177.
Kendrick, B. and DiCosmo, F. with the unitunicate and bitunicate committees of Kananaskis-II. 1979. Teleomorph-anamorph connections in Ascomycetes. In: The whole fungus, the sexual-asexual synthesis, (ed. by Kendrick, B.), pp. 283–410. National Museum of Natural Sciences, National Museums of Canada and the Kananaskis Foundation, Canada.
Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.16: 111–120.
Kirk, P. M. 1985. New or interesting microfungi XIV. Dematiaceous hyphomycetes from Mt Kenya. Mycotaxon23: 305–352.
Kirk, P. M. and Sutton, B. C. 1985. A reassessment of the anamorph genusChaetopsina (Hyphomycetes). Trans. Br. Mycol. Soc.85: 709–717.
Kornerup, A. and Wanscher, J. H. 1978. Methuen handbook of colour, 3rd ed. E. Methuen, London.
Kuthubutheen, A. J. and Nawawi, A. 1988. Two new species ofKionochaeta (Hyphomycetes) andK. ramifera from Malaysia. Trans. Br. Mycol. Soc.90: 437–444.
Lane D. J., Pace B., Olsen, G. J., Stahl, D. A., Sogin, M. L. and Pace, N. P. 1985. Rapid determination of 18S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. USA82: 6955–6959.
Matsushima, T. 1971. Microfungi of the Solomon islands and Papua-New Guinea. Published by the author, Kobe.
Merli, S., Garofano, L., Rambelli, A. and Pasqualetti, M. 1992.Chaetopsina nimbae, a new species of dematiaceous Hyphomycetes. Mycotaxo44: 323–331.
Morgan-Jones, G. 1979. Notes on Hyphomycetes. XXXI.Chaetopsina auburnensis sp. nov. Mycotaxon8: 411–416.
Nishida, H. and Sugiyama, J. 1993. Phylogenetic relationships amongTaphrina, Saitoella, and other higher fungi. Mol. Biol. Evol.10: 431–436.
Nishida, H. and Sugiyama, J. 1994. Archiascomycetes: detection of a major new lineage within the Ascomycota. Mycoscience35: 361–366.
Ogawa, H., Yoshimura, A. and Sugiyama, J. 1997. Polyphyletic origins of species of the anamorphic genusGeosmithia and the relationships of the cleistothecial genera: Evidence from 18S, 5S and 28S rDNA sequence analyses. Mycologia89: 756–771.
Onofri, S. and Zucconi, L. 1991. Scanning electron microscopy of conidiophore development and conidiogenesis inChaetopsina fulva. Mycotaxon41: 451–457.
Pearson, W. R. and Lipman, D. J. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA85: 2444–2448.
Persiani, A. M., Zucconi, L. and Onofri, S. 1984. Hyphomycetes rari o interessanti della foresta tropicale. III. Contributo alla conoscenza del genereChaetopsina Rambelli. Mic. Ital.2: 11–14.
Rambelli, A. 1956.Chaetopsina, nuovo genere di ifali demaziacei. Atti Accad. Sci. dell Ist. Bol.3: 1–6.
Rambelli, A. 1987. A bibliographic reassessment of the genusChaetopsina. Micol. Ital.1: 7–13.
Rambelli, A. and Lunghini, D. 1976. “Chaetopsina ivoriensis” a new species of dematiaceous Hyphomycetes. Giorn. Bot. Ital.110: 253–258.
Rambelli, A. and Lunghini, D. 1979.Chaetopsina species from tropical forest litter. Trans. Br. Mycol. Soc.72: 491–494.
Rambelli, A., Pasqualetti, M. and Lunghini, D. 1991a. Variabilità morfologica in tre ceppi diChaetopsina fulva. Micol. Veget. Mediterranea6: 11–15.
Rambelli, A., Zucconi, L. and Pasqualetti, M. 1991b. Variabilità morfo-dimensionale di due ceppi diChaetopsina fulva su differenti matrici vegetall. Giorn. Bot. Ital.125: 907–917.
Saitou, N. and Nei, M. 1987. The neighhour-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol.4: 406–425.
Samuels, G. J. 1985. Four new species ofNectria and theirChaetopsina anamorphs. Mycotaxon22: 13–32.
Sanger, F., Nicklen, S. and Coulson, A. R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA74: 5463–5467.
Spatafora, J. W. 1995. Ascomal evolution of filamentous ascomycetes: evidence from molecular data. Can. J. Bot.73 (Suppl. 1): S811-S815.
Spatafora, J. W. and Blackwell, M. 1994. Cladistic analysis of partial ssrDNA sequences among unitunicate perithecial ascomycetes and its implications on the evolution of centrum development. In: Ascomycete systematics; problems and perspectives in the nineties, (ed. by Hawksworth, D. L.), pp. 233–242. Plenum Press, New York.
Spatafora, J. W., Mitchell, T. G. and Vilgalys, R. 1995. Analysis of genes coding for small-subunit rRNA sequences in studying phylogenetics of dematiaceous fungal pathogens. J. Clin. Microbiol.33: 1322–1326.
Stackebrandt, E. and Goebel, B. M. 1994. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol.44: 846–849.
Sutton, B. C. and Hodges, C. S. 1976.Eucalyptus microfungi: Some setose hyphomycetes with phialides. Nova Hedwigia27: 343–352.
Taylor, J. W. 1995. Making the Deuteromycota redundant: a practical integration of mitosporic and meiosporic fungi. Can. J. Bot.73 (Suppl. 1): S754-S759.
Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res.22: 4673–4680.
Tubaki, K. and Saito, T. 1969.Endophragmia alternata sp. nov. and other hyphomycetes onPinus leaves in Japan. Trans. Br. Mycol. Soc.52: 477–482.
Untereiner, W. A. 1995. Fruiting studies in species ofCapronia (Herpotrichiellaceae). Antonie van Leeuwenhoek68: 3–17.
Untereiner, W. A., Straus, N. A. and Malloch, D. 1995. A molecular-morphotaxonomic approach to the systematics of the Herpotrichiellaceae and allied black yeasts. Mycol. Res.99: 897–913.
Wingfield, M. J. 1987. A new name forPhialocephala illini. Mycotaxon30: 149–156.
Yotsumoto, Y., Oohara, A. and Mikawa, T. 1995. A convenient method for extraction and purification of DNA from filamentous fungi. Microbiol. Cult. Coll.11: 19–21. (In Japanese).
Zucconi, L. and Rambelli, A. 1993. A new species ofChaetopsina from tropical forest litter. Mycotaxon48: 5–12.
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Okada, G., Takematsu, A. & Takamura, Y. Phylogenetic relationships of the hyphomycete generaChaetopsina andKionochaeta based on 18S rDNA sequences. Mycoscience 38, 409–420 (1997). https://doi.org/10.1007/BF02461681
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DOI: https://doi.org/10.1007/BF02461681