Abstract
Somatic cell hybrids obtained from the fusion of human B lymphocytes and an asparagine synthetase-deficient Chinese hamster ovary cell line were isolated after growth in asparagine-free medium. The human and hamster forms of asparagine synthetase differ significantly in their rate of inactivation at 47.5° C. The asparagine synthetase activity expressed in the hybrids was inactivated at 47.5° C at the same rate as the human form of the enzyme. Karyotypic analysis and analysis for chromosome-specific enzyme markers showed that the structural gene for asparagine synthetase is located on chromosome 7 in humans. The heat-inactivation profile for asparagine synthetase in extracts of hybrids formed between human peripheral leukocytes and a hamster cell line expressing asparagine synthetase activity was intermediate between the two parental types when human chromosome 7 was present, but was identical to the hamster parent when chromosome 7 was absent.
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Arfin, S.M., Cirullo, R.E., Arredondo-Vega, F.X. et al. Assignment of structural gene for asparagine synthetase to human chromosome 7. Somat Cell Mol Genet 9, 517–531 (1983). https://doi.org/10.1007/BF01574256
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DOI: https://doi.org/10.1007/BF01574256