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Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria

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Synthetic DNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1472))

Abstract

Clonetegration is a method for site-specific insertion of DNA into prokaryotic chromosomes, based on bacteriophage integrases. The method combines DNA cloning/assembly and chromosomal integration into a single step, providing a simple and rapid strategy for inserting DNA sequences into bacterial chromosomes.

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Acknowledgements

This work was supported by the Pasteur-Roux Fellowship (L.C.) and Australian Research Council grants DP110100824 and DP110101470 and HFSP grant RGP051 (K.E.S.).

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Authors and Affiliations

  1. Synthetic Biology Group, Institute Pasteur, Paris, 75015, France

    Lun Cui

  2. Department of Molecular and Cellular Biology, School of Biological Science, University of Adelaide, Adelaide, SA, 5005, Australia

    Keith E. Shearwin

Authors
  1. Lun Cui
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  2. Keith E. Shearwin
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Corresponding author

Correspondence to Keith E. Shearwin .

Editor information

Editors and Affiliations

  1. Applied Research Laboratories, The University of Texas at Austin, AUSTIN, Texas, USA

    Randall A. Hughes

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Cui, L., Shearwin, K.E. (2017). Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria. In: Hughes, R. (eds) Synthetic DNA. Methods in Molecular Biology, vol 1472. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6343-0_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6343-0_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6341-6

  • Online ISBN: 978-1-4939-6343-0

  • eBook Packages: Springer Protocols

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