Summary
It could be shown that in uraemic subjects the calciumbinding protein content in duodenal mucosa was 23% less than the corresponding tissue of patients without renal disease. The calcium-binding protein content in uraemic jejunal tissue was even further decreased when compared with normal duodenal tissue. However, there was no normal jejunal tissue available for comparison. The calciumbinding protein was partially purified on Sephadex G-75 and appears by disc electrophoresis as one band, moving rapidly toward the anode. The relationship between these findings and the well known vitamin D resistance in patients with renal disease is discussed.
Zusammenfassung
Duodenum und Jejunum von 2 urämischen Patienten wurden auf ihren Gehalt an calciumbindenden Protein (CaBP) untersucht und mit gepoolten Duodenalstücken von 10 Nierengesunden verglichen. Das CaBP, über Sephadex G-75 teiweise gereinigt, zeigt in der Discelektrophorese eine rasch zur Anode verlaufende Proteinbande. Die Calciumbindungsfähigkeit (45Ca cpm/mg Protein) der Gewebshomogenate wurde mit dem Chelex-100-Test bestimmt. Die spezifische Calciumbindungsaktivität war im urämischen Duodenum um 23% und im urämischen Jejunum um 34% gegenüber dem Duodenum Nierengesunder vermindert.
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Literatur
Avioli, L. V., Birge, S., Lee, S. W., Slatopolsky, E.: The metabolic fate of vitamin D3-3H in chronic renal failure. J. clin. Invest.47, 2239 (1968).
Avioli, L. V., Scott, S., Lee, S. W., De Luca, H. F.: Intestinal calcium absorption: nature of defect in chronic renal disease. Science166, 1154 (1969).
Bearg, R. D., Kimberg, D. V., Gershon, E.: Effect of renal insufficiency on the active transport of calcium by the small intestine. J. clin. Invest.49, 1288 (1970).
Blunt, J. W., De Luca, H. F., Schnoes, H. K.: 25-Hydroxycholecalciferol: a biologically active metabolite of cholecalciferol. Chem. Commun.607, 801 (1968).
Briggs, F. N., Fleishman, M.: Calcium binding by particle-free supernatants of homogenates of skeletal muscle. J. gen. Physiol.49, 131 (1965).
Dent, C. E., Harper, C. M., Philpot, G. R.: The treatment of renal-glomerular osteodystrophy. Quart. J. Med.30, 1 (1961).
Fraser, D. R., Kodicek, E.: Unique biosynthesis by kidney of a biologically active vitamin D metabolite. Nature (Lond.)228, 764 (1970).
Hesch, R.-D., Henning, H. V., Gerlach, W., Scheler, F.: Studies on the intestinal47Calcium absorption in normal persons and patients undergoing peritoneal dialysis, hemodialysis and vitamin D treatment. Proc. of the E.D.T.A., vol. VII, p. 118. 1970: Pitman Medical & Scientific Publishing Co. Ltd. 1907.
Kleeman, C. R., Better, O., Massry, S. G., Maxwell, M. H.: Divalent ion metabolism and osteodystrophy in chronic renal failure. Yale J. Biol. Med.40, 1 (1967).
Liu, S. H., Chu, H. I.: Studies of calcium and phosphorus metabolism with special reference to pathogenesis and effects of dihydrotachysterol (AT10) and iron. Medicine (Baltimore)22, 103 (1943).
Martin, D. M., De Luca, H. F.: Influence of sodium on calcium transport by the rat small intestine. Amer. J. Physiol.216, 1351 (1969).
Menczel, J., Eilon, G., Steiner, A., Karaman, C., Mor, E., Ron, A.: Calcium binding protein in human intestinal mucosa. Israel J. med. Sci.7, 396 (1971).
Muldowney, F. P.: Metabolic bone disease secondary to renal and intestinal disorders. Calif. Med.110, 397 (1969).
Myrtle, J. F., Norman, A. W.: Vitamin D: a cholecalciferol metabolite highly active in promoting intestinal calcium transport. Science171, 79 (1971).
O'Riordan, J. L. H., Page, J., Kerr, D. N. S., Walls, J., Moorhead, J., Crockett, R. E., Franz, H. E., Ritz, E.: Hyperparathyroidism in chronic renal failure and dialysis osteodystrophy. Quart. J. Med.39, 359 (1970).
Piazolo, P., Schleyer, M., Franz, H. E.: Isolation and purification of a calcium-binding-protein from human tissues. Hoppe-Seylers Z. Physiol. Chem.352, 1480 (1971).
Piazolo, P., Schleyer, M., Franz, H. E.: Untersuchungen über die Calciumbindungsfähigkeit von Humanalbumin und einem calciumbindenden Protein der menschlichen Niere. Verhandlg. Deutsch. Ges. Inn. Med.77, 732 (1971).
Ponchon, G., Kennan, A. L., De Luca, H. F.: Activation of vitamin-D by the liver. J. clin. Invest.48, 2032 (1969).
Ritz, E., Jantzen, R.: Vitamin D-Aktivität im Serum urämischer Patienten. Klin. Wschr.47, 1113 (1969).
Schäfer, K., Schäfer, P., Köppe, P., Opitz, A., Höffler, D.: Untersuchungen zur Frage der urämischen Osteopathie: Störungen der intestinalen Calciumresorption in Abhängigkeit von der Nierenfunktion. Dtsch. med. Wschr.93, 1018 (1968).
Shafer, R. B.: Current concepts of calcium absorption. Calif. Med.114, 91 (1971).
Stanbury, S. W., Lumb, G. A.: Metabolic studies of renal osteodystrophy: I. Calcium, phosphorus and nitrogen metabolism in rickets, osteomalacia and hyperparathyroidism complicating chronic uremia and in the adult Fanconi syndrome. Medicine (Baltimore)41, 1 (1962).
Stanbury, S. W.: Bony complications of renal disease. In: Black, D. A. K. (ed.), Renal disease, p. 665. Philadelphia: F. A. Davis Co. 1967.
Taylor, A. N., Wasserman, R. H.: Vitamin D3-induced calcium binding protein: partial purification, electrophoretic visualisation and tissue distribution. Arch. Biochem.119, 536 (1967).
Wasserman, R. H., Taylor, A. N.: Vitamin D-dependent calcium binding protein: Response to some physiological and nutritional variables. J. biol. Chem.243, 3987 (1968).
Wasserman, R. H., Corradino, R. A., Taylor, A. N.: Vitamin D-dependent calcium-binding protein. J. biol. Chem.243, 3978 (1968).
Wasserman, R. H.: Calcium transport by the intestine. Calcif. Tiss. Res.2, 301 (1968).
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Piazolo, P., Schleyer, M. & Franz, H.E. Das calciumbindende Protein in der menschlichen Darmschleimhaut bei Urämie. Klin Wochenschr 50, 603–605 (1972). https://doi.org/10.1007/BF01488778
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DOI: https://doi.org/10.1007/BF01488778