Antonie van Leeuwenhoek

, Volume 59, Issue 3, pp 167–175

Occurrence and significance of precipitating antibodies against thermophilic actinomycetes in the sera of dairy herd workers, Nangali, Delhi

Authors

  • Madhurama Gangwar
    • Department of Medical Mycology, Vallabhbhai Patel Chest InstituteUniversity of Delhi
  • Z. U. Khan
    • Department of Medical Mycology, Vallabhbhai Patel Chest InstituteUniversity of Delhi
  • S. N. Gaur
    • Department of Clinical Research, Vallabhbhai Patel Chest InstituteUniversity of Delhi
  • H. S. Randhawa
    • Department of Medical Mycology, Vallabhbhai Patel Chest InstituteUniversity of Delhi
Article

DOI: 10.1007/BF00580656

Cite this article as:
Gangwar, M., Khan, Z.U., Gaur, S.N. et al. Antonie van Leeuwenhoek (1991) 59: 167. doi:10.1007/BF00580656
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Abstract

The study was prompted by the lack of information on the role of thermophilic actinomycetes in hypersensitivity pneumonitis in India. It reports the occurrence of precipitating antibodies against clinically important thermophilic actinomycetes in the sera of a population sample of dairy herd workers, Nangali, Delhi. Of 112 workers investigated, 28 (25%) showed precipitins againstFaenia rectivirgula, 4 (3.2%) againstSaccharomonospora viridis, 2 againstThermoactinomyces thalpophilus and one each againstT. vulgaris andT. sacchari. The results of enzyme-linked immunosorbent assay (ELISA) indicated that IgG antibody activity againstF. rectivirgula was significantly higher in the symptomatic group than in the asymptomatic group (p<0.05) of workers and the controls (p<0.01). Significant difference inF. rectivirgula IgG activity was also obtained between the precipitin-positive symptomatic group and the precipitin-positive asymptomatic group (p< 0.05). In strong contrast, the IgG antibody activity againstT. thalpophilus was found to be uniformly low. A limited aeromicrobiological sampling of the dairy farm revealedS. viridis (55.8%) to be the commonest species followed byT. vulgaris (19.2%),T. thalpophilus (18.5%),F. rectivirgula (5%) andT. sacchari (15%). On the basis of suggestive clinical and laboratory findings, farmer's lung disease was suspected in four dairy herd workers. A comprehensive clinical evaluation including pulmonary function studies on the dairy herd workers and their long-term follow-up is indicated to determine the extent of respiratory morbidity caused byF. rectivirgula, S. viridis, T. thalpophilus, T. sacchari andT. vulgaris in India.

Key words

precipitating antibodies to thermophilic actinomycetesFaenia rectivirgulaSaccharomonospora viridisdairy herd workersOuchterlony's double diffusion testcounterimmunoelectrophoresisenzyme-linked immunosorbent assay

Copyright information

© Kluwer Academic Publishers 1991