Summary
The cAMP content of the bovine superior cervical ganglion increases rapidly when the tissue is incubated in media containing high concentrations of potassium and theophylline. This effect of high K+ is mimicked by Rb+, Cs+ and NH4 +. The depolarizing substances veratridine and ouabain also cause an increase in the concentration of ganglionic cAMP; the effect of ouabain develops more slowly than that of veratridine. Tetracaine, in high concentrations, reduces the response to potassium, whereas it reduces the response to veratridine at much lower concentrations. Tetrodotoxin, at a concentration that barely affects the response to potassium, prevents the response to veratridine.
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Baker, P. F.: Phosphorus metabolism of intact crab nerve and its relation to the active transport of ions. J. Physiol. (Lond.) 180, 383–423 (1965)
Brown, B. L., Albano, J., Ekins, R. P., Sgherzi, A., Tampion, W.: A simple and sensitive saturation assay method for th measurement of cyclic AMP. Biochem. J. 121, 561–562 (1971)
Chatzkel, S., Zimmermann, I., Berg, A.: Modulation of cyclic AMP synthesis in the cat superior cervical ganglion by short term presynaptic stimulation. Brain Res. 80, 523–526 (1974)
Cramer, H., Johnson, D., Hanbauer, I., Silberstein, S., Kopin, I.: Accumulation of adenosine 3′,5′-monophosphate induced by catecholamines in the rat superior cervical ganglion in vitro. Brain Res. 53, 97–104 (1973)
Elmquist, D., Feldman, D. W.: Spontaneous activity at a mammalian neuromuscular junction in tetrodotoxin. Acta physiol. scand. 64, 475–476 (1965)
Huang, M., Shimizu, H., Daly, J. W.: Accumulation of cyclic AMP in incubated slices of brain tissue. 2. Effects of depolarizing agents, membrane stabilizers, phosphodiesterase inhibitors and adenosine analogs. J. med. Chem. 15, 462–466 (1972)
Kalix, P., McAfee, D., Schorderet, M., Greengard, P.: Pharmacological analysis of synaptically mediated increase in cyclic AMP in rabbit superior cervical ganglion. J. Pharmacol. exp. Ther. 188, 676–687 (1974)
Kebabian, J., Greengard, P.: Dopamine-sensitive adenylcyclase: Possible role in synaptic transmission. Science 174, 1346–1349 (1971)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
McAfee, D. A., Schorderet, M., Greengard, P.: Adenosine 3′,5′-monophosphate in nervous tissue: Increase associated with synaptic transmission. Science 171, 1156–1158 (1971)
Narahashi, T., Moore, W., Scott, W. R.: Tetrodotoxin blockage of sodium conductance increase in lobster giant axons. J. gen. Physiol. 47, 865–8974 (1964)
Roch, P., Kalix, P.: Effect of biogenic amines on the concentration of adenosine 3′,5′-monophosphate in bovine superior cervical ganglion. Neuropharmacology 14, 21–29 (1975a)
Roch, P., Kalix, P.: Adenosine 3′,5′-monophosphate in bovine superior cervical ganglion: Effect of high extracellular potassium. Biochem. Pharmacol. 24, 1293–1296 (1975b)
Schultz, J., Kleefeld, G.: Stimulation of adenosine 3′,5′-monophosphate formation in guinea-pig cerebral cortical slices in a calcium-free medium. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, 289–296 (1975)
Shimizu, H., Creveling, C., Daly, J. W.: Cyclic AMP formation in brain slices: Stimulation by batrachotoxin, ouabain, veratridine and potassium ions. Molec. Pharmacol. 6, 184–188 (1970)
Straub, R. W.: Die Wirkung vin Veratridin und Ionen auf das Ruhepotential markhaltiger Nervenfasern. Helv. physiol. pharmacol. Acta 14, 1–28 (1956)
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Kalix, P., Roch, P. Effect of depolarizing agents on the adenosine-3′,5′-monophosphate content of the bovine superior cervical ganglion. Naunyn-Schmiedeberg's Arch. Pharmacol. 291, 131–137 (1975). https://doi.org/10.1007/BF00500045
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DOI: https://doi.org/10.1007/BF00500045