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Neurophysin and the neurosecretory fibres of the sheep infundibulum

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Summary

A cross-species reactive antiserum was used to study the cellular localization of neurophysin in the sheep hypothalamus and neurohypophysis. The immunofluorescent technique was used to follow the neurosecretory fibres emanating from the magnocellular nuclei to the lower infundibular stem. It was confirmed that neurophysin was present in both the internal and external zone of the infundibulum.

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References

  • Alvarez-Buylla, R., Livett, B. G., Uttenthal, L. O., Hope, D. B., Milton, S. H.: Immunochemical evidence for the transport of neurophysin in the hypothalamo-neurohypophysial system of the dog. Z. Zellforsch. 137, 435–450 (1973).

    Google Scholar 

  • Dellman, H.-D., Rodríguez, E. M.: Investigations on the hypothalamo-neurohypophysial neurosecretory system of the grass frog (Rana pipiens) after transection of the proximal neurohypophysis. III. Ultrastructure and hormone content of the distal stump. In: Aspects of Neuroendocrinology. Proceedings Vth International Symposium on Neurosecretion, Kiel (1969) (ed. by Bargmann, W. and Scharrer, B.), p. 124–139. Berlin-Heidelberg-New York: Springer 1970

    Google Scholar 

  • Ellis, H. K., Watkins, W. B.: Fractionation of guinea-pig neurohypophysial proteins and the identification of a single major neurophysin. Proc. Endocrine Soc. Australia 15, 40 (1972)

    Google Scholar 

  • Ellis, H. K., Watkins, W. B., Evans, J. J.: Distribution of soluble proteins in the mammalian neurohypophysis and their cross-reactivity with anti-neurophysin. J. Endocr. 55, 565–575 (1972)

    Google Scholar 

  • Evans, J. J., Watkins, W. B.: Localization of neurophysin in the neurosecretory elements of the hypothalamus and neurohypophysis of the normal and osmotically stimulated guinea-pig as demonstrated by immunofluorescence histochemical technique. Z. Zellforsch. 145, 39–55 (1973)

    Google Scholar 

  • Flament-Durand, J.: Contribution à l'étude de la neurosécrétion chez le rat par la méthode autoradiographique. In: Neurosecretion (ed. by Stutinsky, F.), p. 60–76. Berlin-Heidelberg-New York: Springer 1967

    Google Scholar 

  • Flament-Durand, J., Dustin, P.: Studies on the transport of secretory granules in the magnocellular hypothalamic neurons. I. Action of colchicine on axonal flow and neurotubules in the paraventricular nuclei. Z. Zellforsch. 130, 440–454 (1972)

    Google Scholar 

  • Haymaker, W.: Hypothalamo-pituitary neural pathways and the circulatory system of the pituitary. In: The hypothalamus (ed. by Haymaker, W., Anderson, E. and Nauta, W.J.H.) p. 219–250. Springfield, Illinois: Charles C. Thomas 1969

    Google Scholar 

  • Heller, H., Hasan, S. H., Saifi, A. O.: Antidiuretic activity in cerebrospinal fluid. J. Endocr. 41, 273–280 (1968)

    Google Scholar 

  • Hollenberg, M. D., Hope, D. B.: The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Crystallization of neurophysin-I and -II as complexes with (8-arginine)-vasopressin. Biochem. J. 106, 557–564 (1968)

    Google Scholar 

  • Jones, C. W., Pickering, B. T.: Intra-axonal transport and turnover of neurohypophysial hormones in the rat. J. Physiol. (Lond.) 227, 553–564 (1972)

    Google Scholar 

  • Kawamura, A.: Fluorescent antibody techniques and their application. Manchester: University Park Press 1969

    Google Scholar 

  • Knaggs, G. S., Tindal, J. S., Turvey, A.: Paraventricular-hypophysial neurosecretory pathways in the guinea-pig. J. Endocr. 50, 153–162 (1971)

    Google Scholar 

  • Knowles, F., Anand Kumar, T. C.: Structure changes, related to reproduction, in the hypothalamus and in the pars tuberalis of the rhesus monkey. Phil. Trans. B 256, 357–375 (1969)

    Google Scholar 

  • Knowles, F., Vollrath, L.: Neurosecretory innervation of the pituitary of the eels Anguilla and Conger I. The structure and ultrastructure of the neurointermediate lobe under normal and experimental conditions. Phil. Trans. B 250, 311–327 (1966)

    Google Scholar 

  • Kobayashi, H., Matsui, T., Ishii, S.: Functional electron microscopy of the hypothalamic median eminence. Int. Rev. Cytol. 29, 281–381 (1970)

    Google Scholar 

  • Livett, B. G., Parry, H. B.: Accumulation of neurophysin in the median eminence and the cerebellum of sheep with natural scrapie. Brit. J. Pharm. (Lond.) 43, 423p-424p (1971)

    Google Scholar 

  • Livett, B. G., Parry, H. B.: The distribution of vasopressin and neurophysin in the hypothalamo-distal-neurohypophysial and hypothalamo-infundibular neurosecretory systems of normal and scrapie-affected sheep. J. Physiol. (Lond.) 230, 20P-22P (1973)

    Google Scholar 

  • Livett, B. G., Uttenthal, L. O., Hope, D. B.: Localization of neurophysin-II in the hypothalamo-neurohypophysial system of the pig by immunofluorescence histochemistry. Phil. Trans. B 261, 371–378 (1971)

    Google Scholar 

  • Nairn, R. C.: Fluorescent protein tracing, 3rd ed. London: Livingstone 1969

    Google Scholar 

  • Norström, A., Sjöstrand, J.: Transport and turnover of neurohypophysial proteins of the rat. J. Neurochem. 18, 2007–2016 (1971)

    Google Scholar 

  • Norström, A., Sjöstrand, J., Livett, B. G., Uttenthal, L. O., Hope, D. B.: Electrophoretic and immunologic characterization of rat neurophysin. Biochem. J. 122, 671–676 (1971)

    Google Scholar 

  • Parry, H. B., Livett, B. G.: A new hypothalamic pathway to the median eminence containing neurophysin and its hypertrophy in sheep with natural scrapie. Nature (Lond.) 242, 63–65 (1973)

    Google Scholar 

  • Rodríguez, E. M.: Morphological and functional relationships between the hypothalamo-neurohypophysial system and cerebrospinal fluid. In: Aspects of Neuroendocrinology Proceedings. Vth International Symposium on Neurosecretion, Kiel 1969 (ed. by Bargmann, W., Scharrer, B.), p. 352–365. Berlin-Heidelberg-New York: Springer 1970

    Google Scholar 

  • Rodríguez, E. M., Heller, H.: Antidiuretic activity and ultrastructure of the toad choroid plexus. J. Endocr. 46, 83–91 (1970)

    Google Scholar 

  • Szentágothai, J.: The parvicellular neurosecretory system. In: Progr. in brain. res. (ed. by Bargmann, W., Schadé, J. P.), vol. 5, p. 135–146. Amsterdam: Elsevier 1964

    Google Scholar 

  • Talanti, S., Attila, U., Kekki, M.: The kinetics of 35S-labelled cysteine in the hypothalamic-hypophyseal tract of the rat, studied by autoradiography. Z. Zellforsch. 124, 342–353 (1972)

    Google Scholar 

  • Uttenthal, L. O., Hope, D. B.: The isolation of three neurophysins from porcine posterior pituitary lobes. Biochem. J. 116, 899–909 (1970)

    Google Scholar 

  • Vorherr, H., Bradbury, M.W.B., Hoghoughi, M., Kleeman, C. R.: Antidiuretic hormone in cerebrospinal fluid. Endogenous and exogenous changes in its blood level. Endocrinology 83, 246–250 (1968)

    Google Scholar 

  • Watkins, W. B.: Neurophysins of the sheep. Biochem. J. 126, 759–760 (1972)

    Google Scholar 

  • Watkins, W. B.: The isolation and characterization of three neurophysins of the sheep. J. Endocr. (in press) (1973)

  • Watkins, W. B.: Immunohistochemical demonstration of neurophysin in the hypothalamo-neurohypophysial system. Int. Rev. Cytol. (in press) (1974)

  • Watkins, W. B., Ellis, H. K.: The designation of the major guinea-pig neural lobe-protein as a neurophysin. J. Endocr. (in press) (1973)

  • Watkins, W. B., Evans, J. J.: Demonstration of neurophysin in the hypothalamo-neurohypophysial system of the normal and dehydrated rat by the use of cross-species reactive anti-neurophysins. Z. Zellforsch. 131, 149–170 (1972)

    Google Scholar 

  • Zimmerman, E. A., Hsu, K. C., Robinson, A. G., Carmel, P. W., Frantz, A. G., Tannebaum, M.: Studies of neurophysin secreting neurons with immunoperoxidase techniques employing antibody to bovine neurophysin. I. Light microscopic findings in monkey and bovine tissue. Endocrinology 92, 931–940 (1973)

    Google Scholar 

  • Zimmerman, E. A., Robinson, A. G., Carmel, P. W., Hsu, K. C., Tannebaum, M., Frantz, A. G.: Hypothalamic and cerebrospinal fluid neurophysins. Clin. Res. 20, 466 (1972)

    Google Scholar 

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This work was supported by a research grant from the New Zealand Medical Research Council.

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Watkins, W.B. Neurophysin and the neurosecretory fibres of the sheep infundibulum. Z.Zellforsch 145, 471–478 (1973). https://doi.org/10.1007/BF00306719

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