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Histochemical localisation of prostaglandin synthetase

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Summary

A method has been developed for the detection of the prostaglandin synthesizing enzyme system in tissue sections. The localisation of the enzyme is indicated by a brown staining when cryotome sections are incubated with the prostaglandin precursor 8,11,14-eicosatrienoic acid in the presence of 3,3′-diaminobenzidine and KCN. The method is shown to be specific for prostaglandin synthetase. Results obtained with sheep vesicular glands and rabbit kidney are presented.

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Authors and Affiliations

  1. Unilever Research, Vlaardingen, The Netherlands

    F. H. A. Janszen & D. H. Nugteren

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  1. F. H. A. Janszen
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  2. D. H. Nugteren
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Janszen, F.H.A., Nugteren, D.H. Histochemical localisation of prostaglandin synthetase. Histochemie 27, 159–164 (1971). https://doi.org/10.1007/BF00284957

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