Skip to main content
SpringerLink
Log in

Striatonigral involvement following transient focal cerebral ischemia in the rats: an immunohistochemical study on a reversible ischemia model

  • Regular Papers
  • Published:
Acta Neuropathologica Aims and scope Submit manuscript

Summary

A topographical and cellular immunohistochemical analysis was performed on the striatonigral system of rats with unilateral, reversible middle cerebral artery (MCA) occlusion. Antibodies to calcineurin (CaN), parvalbumin (PV), choline acetyltransferase (ChAT) and glial fibrillary acidic protein (GFAP) were used in this study. Sixty days after the operation, the ipsilateral striatum showed a characteristic cell type-specific injury in the dorsolateral part of the nucleus (i.e., non-limbic striatum): a marked reduction in the number of medium-sized spinous neurons expressing CaN immunoreactivity and a selective sparing of PV-and ChAT-positive interneurons. There was also a marked depletion of striatonigral afferents visualized by CaN immunostaining in the lateral portion of the substantia nigra pars reticulata, which is considered to be implicated with motor function. In addition, it was noted that such striatonigral involvement was accompanied by marked gliosis showing strong GFAP immunolabeling. The present data suggest that rats with reversible MCA occlusion can be a useful animal model for studying cell type-specific ischemic injury and subdivisional involvement of the striatonigral pathway as a part of the cortico-subcortical loop subserving motor function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Beal MF, Kowall NW, Ellison DW, Mazurek MF, Schwartz KJ, Martin JB (1986) Replication of the neurochemical characteristics of Huntington's disease by quinolinic acid. Nature 321:168–171

    Google Scholar 

  2. Beal ME, Kowall NW, Schwartz KJ, Ferrante RJ, Martin JB (1988) Model of Huntington's disease. Science 241:475

    Google Scholar 

  3. Beal MF, Kowall NW, Schwartz KJ, Ferrante RJ, Martin JB (1989) Differential sparing of somatostatin-neuropeptide Y and cholinergic neurons following striatal excitotoxin lesions. Synapse 3:38–47

    Google Scholar 

  4. Bolam JP, Wainer BH, Smith AD (1984) Characterization of cholinergic neurons in the rat neostriatum: a combination of choline acetyltransferase immunocytochemistry, Golgi impregnation and electron microscopy. Neuroscience 12:711–718

    Google Scholar 

  5. Borit A, Rubinstein LJ, Urich H (1975) The striatonigral degenerations: putaminal pigments and nosology. Brain 98:101–112

    Google Scholar 

  6. Coyle JT, Schwarcz R (1976) Lesions of striatal neurons with kainic acid provides model for Huntington's chorea. Nature 263:244–246

    CAS  PubMed  Google Scholar 

  7. Crain BJ, Westerkam WD, Harrison AH, Nadler JV (1988) Selective neuronal death after transient forebrain ischemia in the Mongorian gerbil: a silver-impregnation study. Neuroscience 27:387–402

    Google Scholar 

  8. DeLong MR, Georgopoulos AP, Crutcher MD, Mitchell SJ, Richardson RT, Alexander GE (1984) Functional organization of the basal ganglia: contributions of single-cell recording studies. Ciba Found Symp 107:64–78

    Google Scholar 

  9. Evarts EV, Kimura M, Wurtz RH (1984) Behavioral correlates of activity in the basal ganglia neurons. Trend Neurosci 7:447–453

    Google Scholar 

  10. Ferrante RJ, Kowall NW, Beal MF, Richardson EP Jr, Bird ED, Martin JB (1985) Selective sparing of a class of striatal neurons in Huntington's disease. Science 230:561–563

    Google Scholar 

  11. Ferrante RJ, Kowall NW, Beal MF, Martin JB, Bird ED, Richardson EP Jr (1987) Morphologic and histochemical characteristics of a spared set of striatal neurons in Huntington's disease. J Neuropathol Exp Neurol 46:12–27

    Google Scholar 

  12. Ferrante RJ, Beal MF, Kowall NW et al (1987) Sparing of acetylcholinesterase-containing striatal neurons in Huntington's disease. Brain Res 411:162–166

    Google Scholar 

  13. Freund F, Buzsaki G, Leon A, Baimbridge KG, Somogyi P (1990) Relationship for neuronal vulnerability and calcium binding protein immunoreactivity in ischemia. Exp Brain Res 83:55–66

    Google Scholar 

  14. Gerfen CR, Baimbridge KG, Miller JJ (1985) The neostriatal mosaic: complementary distribution of calcium-binding protein and parvalbumin in the basal ganglia of the rat and monkey. Proc Natl Acad Sci USA 82:8780–8784

    Google Scholar 

  15. Goto S, Matsukado Y, Miyamoto E, Yamada M (1987) Morphological characterization of the rat striatal neurons expressing calcineurin immunoreactivity. Neuroscience 22:189–201

    Google Scholar 

  16. Goto S, Hirano A, Rojas-Corona RR (1989) Calcineurin immunoreactivity in striatonigral degeneration. Acta Neuropathol 78:65–71

    Google Scholar 

  17. Goto S, Hirano A, Matsumoto S (1989) Subdivisional involvement of nigro-striatal loop in idiopathic Parkinson's disease and striatonigral degeneration. Ann Neurol 26:766–770

    Google Scholar 

  18. Goto S, Hirano A, Matsumoto S (1990) Immunohistochemical study of striatal efferents and nigral dopaminergic neurons in parkinsonism-dementia complex on Guam in comparison with those in Parkinson's and Alzheimer's diseases. Ann Neurol 27:520–527

    Google Scholar 

  19. Goto S, Hirano A, Matsumoto S (1990) Met-enkephalin immunoreactivity in the basal ganglia in Parkinson's disease and striatonigral degeneration. Neurology 40:1051–1056

    Google Scholar 

  20. Goto S, Nagahiro S, Ushio Y, Hofer W (1992) A simple enhancement method for the silver-gold-intensified diaminobenzidine reaction in the light microscopic immunoperoxidase technique. J Histochem Cytochem 40:1423–1425

    Google Scholar 

  21. Ito H, Goto S, Sakamoto S, Hirano A (1992) Calbindin-D28K in the basal ganglia of patients with parkinsonism. Ann Neurol 32:543–550

    Google Scholar 

  22. Kirino T, Tamura A, Sano K (1984) Delayed neuronal death in the rat hippocampus following transient forebrain ischemia. Acta Neuropathol (Berl) 64:139–147

    Google Scholar 

  23. Koizumi J, Yoshida Y, Nakazawa T, Ooneda G (1986) Experimental studies of ischemic brain edema. I. A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area. Jpn J Stroke 8:1–7

    Google Scholar 

  24. Longa EZ, Weinstein PR, Carlson S, Cummins R (1989) Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 20:84–91

    Google Scholar 

  25. McGeer PL, McGeer EG (1976) Duplication of biochemical changes of Huntington's chorea by intrastriatal injections of glutamic and kainic acids. Nature 263:517–519

    Google Scholar 

  26. Meldrum BS (1985) Excitatory amino acids and anoxic/ischemic brain damage. Trend Neurosci 8:47–48

    Google Scholar 

  27. Nakano S, Kogure K, Fujikura H (1990) Ischemia-induced slowly progressive neuronal damage in the rat brain. Neuroscience 38:115–124

    Google Scholar 

  28. Nauta WJH, Domesick VB (1984) Afferent and efferent relationships of the basal ganglia. Ciba Found Symp 107:3–23

    Google Scholar 

  29. Petito CK, Pulsinelli WA (1984) Sequential development of reversible and irreversible neuronal damage following cerebral ischemia. J Neuropathol Exp Neurol 43:141–153

    Google Scholar 

  30. Pulsinelli WA, Brierley JB, Plum F (1982) Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann Neurol 11:491–498

    Google Scholar 

  31. Rothman SM, Olney JW (1986) Glutamate and pathophysiology of hypoxic-ischemic brain damage. Ann Neurol 19:105–111

    Google Scholar 

  32. Smith M-L, Auer RN, Siejo BK (1984) The density and distribution of ischemic brain injury in the rat following 2–10 min of forebrain ischemia. Acta Neuropathol (Berl) 64:319–332

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Cerebrovascular Disorders and Neuroscience, Department of Neurosurgery, Kumamoto University Medical School, 860, Kumamoto, Japan

    Satoshi Goto, Shinji Nagahiro, Kojiro Korematsu & Yukitaka Ushio

Authors
  1. Satoshi Goto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shinji Nagahiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kojiro Korematsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yukitaka Ushio
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education and Culture of Japan

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goto, S., Nagahiro, S., Korematsu, K. et al. Striatonigral involvement following transient focal cerebral ischemia in the rats: an immunohistochemical study on a reversible ischemia model. Acta Neuropathol 85, 515–520 (1993). https://doi.org/10.1007/BF00230491

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00230491

Key words

Search

Navigation