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Neuronal loss and gliosis in the rat striatum subjected to 15 and 30 minutes of middle cerebral artery occlusion

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Abstract

Selective neuronal death or loss in certain brain regions has been well characterized in animal models of transient global cerebral ischemia. However, selective neuronal death in transient focal cerebral ischemia needs more investigation. Therefore, in this study, we studied selective neuronal death in the striatum (caudate putamen) of rats subjected to 15 or 30 min middle cerebral artery occlusion (MCAO). Neuronal death occurred in the dorsolateral field, not in the medial field in 30 min, not 15 min, MCAO-operated rats 5 days after MCAO using neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In this group, immunoreactivity of glial fibrillary acidic protein in astrocytes was hardly shown in the dorsolateral field, although the immunoreactivity increased in the medial field. In addition, immunoreactivity of ionized calcium binding adapter molecule 1 in microglia was dramatically increased in the dorsolateral, not in the medial, field only in 30 min MCAO-operated rats. Briefly, these results show that at least 30 min of MCAO can evoke selective neuronal death, astrocytic dysfunction and microglial activation in the dorsolateral field of the rat striatum and suggest that a rat model of 30 min MCAO can be used to investigate mechanisms of neuronal death and gliosis following brief transient focal cerebral ischemic events for acute transient ischemic attack.

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Acknowledgements

This research was supported by the Bio-Synergy Research Project (NRF-2015M3A9C4076322) of the Ministry of Science, ICT and Future Planning through the National Research Foundation (NRF) of Korea, Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03030161) and by Basic Science Research Program through the NRF of Korea funded by the Ministry of Science, ICT &Future Planning (NRF-2017R1A2B4008403).

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  1. Joon Ha Park and Jeong Hwi Cho have contributed equally to this article.

Authors and Affiliations

  1. Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon, 24252, Republic of Korea

    Joon Ha Park, Ji Hyeon Ahn & Soo Young Choi

  2. Department of Neurobiology, School of Medicine, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Gangwon, 24341, Republic of Korea

    Jeong Hwi Cho, Tae-Kyeong Lee, Jae-Chul Lee, Bich Na Shin & Moo-Ho Won

  3. Department of Surgery, School of Medicine, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Gangwon, 24341, Republic of Korea

    Seongkweon Hong

  4. Department of Radiology, School of Medicine, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Gangwon, 24341, Republic of Korea

    Yong Hwan Jeon

  5. Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Gangwon, 24341, Republic of Korea

    Young-Myeong Kim

  6. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, 1 Gwanak-ro, Seoul, 08826, Republic of Korea

    In Koo Hwang

  7. Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, 59 Daesagwan-ro, Seoul, 04401, Republic of Korea

    Young Joo Lee

  8. Department of Food Science and Nutrition, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon, 24252, Republic of Korea

    Il Jun Kang

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  1. Joon Ha Park
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Correspondence to Moo-Ho Won or Il Jun Kang.

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Park, J.H., Cho, J.H., Ahn, J.H. et al. Neuronal loss and gliosis in the rat striatum subjected to 15 and 30 minutes of middle cerebral artery occlusion. Metab Brain Dis 33, 775–784 (2018). https://doi.org/10.1007/s11011-018-0192-8

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  • DOI: https://doi.org/10.1007/s11011-018-0192-8

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