Skip to main content
SpringerLink
Log in

Biodegradation of diazo reactive dye Navy blue HE2R (Reactive blue 172) by an isolated Exiguobacterium sp. RD3

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

The diazo reactive dye Navy blue HE2R (50 mg/L) was decolorized up to 91.2% within 48 h at static condition by the Exiguobacterium sp. isolated from the dyestuff contaminated soil, collected from the textile industrial area Solapur, India. It showed ability to decolorize seven different reactive textile dyes. Maximum decolorization was observed at 30°C and pH 7. The presence and significant increase in the activity of enzymes lignin peroxidase, laccase, and azoreductase indicated prominent role of these enzymes in the decolorization of Navy blue HE2R. The degradation metabolites were analyzed by UV-Vis spectroscopy, TLC, HPLC, and FTIR spectroscopy. A possible pathway for biodegradation of this diazo reactive dye was proposed with the help of GC-MS analysis. The phytotoxicity studies confirmed the environmentally safe nature of degradation products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Moosvi, S., H. Keharia, and D. Madamwar (2005) Decolourization of textile dye reactive violet 5 by a newly isolated bacterial consortium RVM 11.1. World J. Microbiol. Biotechnol. 21: 667–672.

    Article  CAS  Google Scholar 

  2. Knight, A. W., P. O. Keenan, N. J. Goddard, P. R. Fielden, and R. M. Walmsley (2004) A yeast-based cytotoxicity and genotoxicity assay for environmental monitoring using novel portable instrumentation. J. Environ. Monit. 6: 71–79.

    Article  CAS  Google Scholar 

  3. Jadhav, J. P., G. K. Parshetti, S. D. Kalme, and S. P. Govindwar (2007) Decolourization of azo dye methyl red by Saccharomyces cerevisiae MTCC 463. Chemosphere 68: 394–400.

    Article  CAS  Google Scholar 

  4. Anjaneyulu, Y., N. S. Chary, and D. S. S. Raj (2005) Decolourization of industrial effluents — available methods and emerging technologies — a review. Rev. Environ. Sci. Biotehnol. 4: 245–273.

    Article  CAS  Google Scholar 

  5. Hatvani, N. and I. Mecs (2001) Production of laccase and manganese peroxidase by Lentinus edodes on malt-containing by-product of the brewing process. Process Biochem. 37: 491–496.

    Article  Google Scholar 

  6. Shanmugam, V., M. Kumari, and K. D. Yadav (1999) n-Propanol as a substrate for assaying the lignin peroxidase activity of Phanerochaete chrysoporium. Indian J. Biochem. Biophys. 36: 39–43.

    CAS  Google Scholar 

  7. Zhang, X. and W. H. Flurkey (1997) Phenoloxidases in Portabella mushrooms. J. Food Sci. 62: 97–100.

    Article  CAS  Google Scholar 

  8. Salokhe, M. D. and S. P. Govindwar (2003) Inducibility of biotransformation enzymes in Serratia marcescens. World J. Microbiol. Biotechnol. 19: 199–200.

    Article  CAS  Google Scholar 

  9. Heinfling, A., M. Bergbauer, and U. Szewzyk (1997) Biodegradation of azo and phthalocyanine dyes by Trametes versicolor and Bjerkandera adusta. Appl. Microbiol. Biotechnol. 48: 261–266.

    Article  CAS  Google Scholar 

  10. Jadhav, J. P. and S. P. Govindwar (2006) Biotransformation of malachite green by Saccharomyces cerevisiae MTCC 463. Yeast 23: 315–323.

    Article  CAS  Google Scholar 

  11. Bustard, M., G. McMullan, and A. P. McHale (1998) Biosorption of textile dyes by biomass derived from Kluyveromyces marxianus IMB3. Bioprocess Eng. 19: 427–430.

    CAS  Google Scholar 

  12. Machado, K. M. G., L. C. A. Compart, R. O. Morais, L. H. Rosa, and M. H. Santos (2006) Biodegradation of reactive textile dyes by basidiomycetous fungi from Brazilian ecosystems. Braz. J. Microbiol. 37: 481–487.

    CAS  Google Scholar 

  13. Kim, S. J., M. J. Kim, and M. Shoda (2006) Decolorization of dye and molasses by continuous and semi-continuous jar-fermentor cultures of Geotrichum candidum Dec 1. Biotechnol. Bioprocess Eng. 11: 306–312.

    Article  CAS  Google Scholar 

  14. Bhatt, N., K. C. Patel, H. Keharia, and D. Madamwar (2005) Decolorization of diazo-dye Reactive Blue 172 by Pseudomonas aeruginosa NBAR12. J. Basic Microbiol. 45: 407–418.

    Article  CAS  Google Scholar 

  15. Dutta, K., S. Bhattacharjee, B. Chaudhuri, and S. Mukhopadhyay (2002) Chemical oxidation of C. I. Reactive Red 2 using Fenton-like reactions. J. Environ. Monit. 4: 754–760.

    Article  CAS  Google Scholar 

  16. Keck, A., J. Klein, M. Kudlich, A. Stolz, H. J. Knackmuss, and R. Mattes (1997) Reduction of azo dyes by redox mediators originating in the naphthalenesulfonic acid degradation pathway of Sphingomonas sp. strain BN6. Appl. Environ. Microbiol. 63: 3684–3690.

    CAS  Google Scholar 

  17. Coughlin, M. F., B. K. Kinkle, and P. L. Bishop (1999) Degradation of azo dyes containing aminonaphthol by Sphingomonas sp. strain 1CX. J. Ind. Microbiol. Biotechnol. 23: 341–346.

    Article  CAS  Google Scholar 

  18. Liu, G.-F., J.-T. Zhou, J. Wang, Z.-Y. Song, and Y.-Y. Qv (2006) Bacterial decolorization of azo dyes by Rhodopseudomonas palustris. World J. Microbiol. Biotechnol. 22: 1069–1074.

    Article  CAS  Google Scholar 

  19. Chen, K. C., W. T. Huang, J. Y. Wu, and J. Y. Houng (1999) Microbial decolorization of azo dyes by Proteus mirabilis. J. Ind. Microbiol. Biotechnol. 23: 686–690.

    Article  CAS  Google Scholar 

  20. Ren, S. Z., J. Guo, G. Q. Zeng, and G. P. Sun (2006) Decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain. Appl. Microbiol. Biotechnol. 72: 1316–1321.

    Article  CAS  Google Scholar 

  21. Lucas, M. S., C. Amaral, A. Sampaio, J. A. Peres, and A. A. Dias (2006) Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila. Enzyme Microb. Technol. 39: 51–55.

    Article  CAS  Google Scholar 

  22. Song, Z.-Y., J.-T. Zhou, J. Wang, B. Yan, and C.-H. Du (2003) Decolorization of azo dyes by Rhodobacter sphaeroides. Biotechnol. Lett. 25: 1815–1818.

    Article  CAS  Google Scholar 

  23. Hong, Y., M. Xu, J. Guo, Z. Xu, X. Chen, and G. Sun (2007) Respiration and growth of Shewanella decolorationis S12 with an azo compound as the sole electron acceptor. Appl. Environ. Microbiol. 73: 64–72.

    Article  CAS  Google Scholar 

  24. Goszczynski, S., A. Paszczynski, M. B. Pasti-Grigsby, R. L. Crawford, and D. L. Crawford (1994) New pathway for degradation of sulfonated azo dyes by microbial peroxidases of Phanerochaete chrysosporium and Streptomyces chromofuscus. J. Bacteriol. 176: 1339–1347.

    CAS  Google Scholar 

  25. Anderson, C. R. and G. M. Cook (2004) Isolation and characterization of arsenate-reducing bacteria from arsenic-contaminated sites in New Zealand. Curr. Microbiol. 48: 341–347.

    Article  CAS  Google Scholar 

  26. Guivarch, E., N. Oturan, and M. A. Oturan (2003) Removal of organophosphorus pesticides from water by electrogenerated Fenton’s reagent. Environ. Chem. Lett. 1: 165–168.

    Article  CAS  Google Scholar 

  27. Chen, H., S. L. Hopper, and C. E. Cerniglia (2005) Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein. Microbiology 151: 1433–1441.

    Article  CAS  Google Scholar 

  28. Mali, P. L., M. M. Mahajan, D. P. Patil, and M. V. Kulkarni (2000) Biodecolorization of members of triphenylmethane and azo groups of dyes. J. Sci. Ind. Res. 59: 221–224.

    CAS  Google Scholar 

  29. Junnarkar, N., D. Murty, N. Bhatt, and D. Madamwar (2006) Decolorization of diazo dye Direct Red 81 by a novel bacterial consortium. World J. Microbiol. Biotechnol. 22: 163–168.

    Article  CAS  Google Scholar 

  30. Haug, W., A. Schmidt, B. Nortemann, D. C. Hempel, A. Stolz, and H. J. Knackmuss (1991) Mineralization of the sulfonated azo dye Mordant Yellow 3 by a 6-aminona-phthalene-2-sulfonate-degrading bacterial consortium. Appl. Environ. Microbiol. 57: 3144–3149.

    CAS  Google Scholar 

  31. Sayan, E. (2006) Optimization and modeling of decolorization and COD reduction of reactive dye solutions by ultrasound-assisted adsorption. Chem. Eng. J. 119: 175–181.

    Article  CAS  Google Scholar 

  32. Parshetti, G., S. Kalme, G. Saratale, and S. Govindwar (2006) Biodegradation of malachite green by Kocuria rosea MTCC 1532. Acta Chim. Slov. 53: 492–498.

    CAS  Google Scholar 

  33. Husain, Q. (2006) Potential applications of the oxidoreductive enzymes in the decolorization and detoxification of textile and other synthetic dyes from polluted water: a review. Crit. Rev. Biotechnol. 26: 201–221.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Shivaji University, Kolhapur, 416 004, India

    R. S. Dhanve, U. U. Shedbalkar & J. P. Jadhav

Authors
  1. R. S. Dhanve
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. U. U. Shedbalkar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. P. Jadhav
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. P. Jadhav.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dhanve, R.S., Shedbalkar, U.U. & Jadhav, J.P. Biodegradation of diazo reactive dye Navy blue HE2R (Reactive blue 172) by an isolated Exiguobacterium sp. RD3. Biotechnol Bioproc E 13, 53–60 (2008). https://doi.org/10.1007/s12257-007-0165-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-007-0165-y

Keywords

Search

Navigation