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Microsatellite markers reveal high genetic diversity in date palm (Phoenix dactylifera L.) germplasm from Sudan

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Abstract

Genetic diversity in date palm germplasm from Sudan representing 37 female and 23 male accessions was investigated using 16 loci of microsatellite (SSR) primers. Eight female accessions from Morocco were included as reference material. The tested SSR markers showed a high level of polymorphism. A total of 343 alleles were detected at the 16 loci. The number of alleles per marker ranged from 14 to 44 with an average of 21.4 per locus. A high level of expected heterozygosity was observed among Sudan cultivars (0.841), Morocco cultivars (0.820) and male accessions (0.799). The results indicate that the genetic groups of the Sudan cultivars and/or males do not follow a clear geographic pattern. However, the morocco group showed significant differentiation in relation to the Sudan groups, as measured by F ST values and genetic distances. The effect of the methods of pollination and cultivar selection on the genetic structure was clearly detected by the weak clustering association that was observed for the majority of accessions originating from Sudan and Morocco as well. This suggests the need for further investigation on the genetic diversity of Sudanese date palm germplasm. A deeper insight will be revealed by a detailed analysis of populations originating from different geographic locations.

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Acknowledgements

We are sincerely grateful to Prof. Olavi Luukkanen for continuous support during this study and Prof. Mohammed Baaziz from Cadi Ayyad University in Morocco for guidance during collection of samples from Morocco. This study was funded by the Islamic Development Bank (IDB), Emil Aaltonen Foundation and Academy of Finland (Project: 210766).

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  1. Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    Sakina Elshibli & Helena Korpelainen

  2. Viikki Tropical Resources Institute (VITRI), University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    Sakina Elshibli

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  1. Sakina Elshibli
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Correspondence to Sakina Elshibli.

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Elshibli, S., Korpelainen, H. Microsatellite markers reveal high genetic diversity in date palm (Phoenix dactylifera L.) germplasm from Sudan. Genetica 134, 251–260 (2008). https://doi.org/10.1007/s10709-007-9232-8

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  • DOI: https://doi.org/10.1007/s10709-007-9232-8

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