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Genetic Analysis of Egyptian Date (Phoenix dactylifera L.) Accessions Using AFLP Markers

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Abstract

Forty-seven samples of date palm (Phoenix dactylifera L.) collected from eight locations in Egypt were studied using four sets of amplified fragment length polymorphism (AFLP) markers with near infrared fluorescence labeled primers. These samples belonged to 21 named accessions and 9 of unknown pedigrees. A total of 350 bands were scored and 233 (66.6%) were polymorphic. Twenty-seven Egyptian accessions and ‘Medjool’and ‘Deglet Noor’accessions from California could beclassified into the major cluster. This major cluster may represent a major group of date palm germplasm in North Africa. There were four other clusters, each containing one or two accessions. The variety ‘Halawy’and one accession of unknown provenance were most likely from hybridization between two clusters. Six groups of accessions of which had the same names, revealed similar but not identical AFLP profiles suggesting these accessions might derive from seedlings rather thanthrough clonal offshoot propagation.

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Abbreviations

AFLPs:

amplified fragment length polymorphism

UPGMA:

unweighted pair group method of the arithmetic average

PCA:

principal coordinated analysis

PCR:

polymerase chain reaction

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Authors and Affiliations

  1. Tropical Fruit and Date Palm Research Department, Horticultural Research Institute, 9 Cairo University Street, Orman, Giza, Egypt

    Ashraf M. El-Assar & Chih-Cheng T. Chao

  2. National Clonal Germplasm Repository for Citrus and Dates, USDA-ARS, 1060 Martin Luther King Boulevard, 92507, Riverside, CA , USA

    Robert R. Krueger

  3. Department of Botany and Plant Sciences, University of California-Riverside, 92521, Riverside, CA, USA

    Pachanoor S. Devanand

Authors
  1. Ashraf M. El-Assar
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  2. Robert R. Krueger
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  3. Pachanoor S. Devanand
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  4. Chih-Cheng T. Chao
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Correspondence to Chih-Cheng T. Chao.

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El-Assar, A.M., Krueger, R.R., Devanand, P.S. et al. Genetic Analysis of Egyptian Date (Phoenix dactylifera L.) Accessions Using AFLP Markers. Genet Resour Crop Evol 52, 601–607 (2005). https://doi.org/10.1007/s10722-004-0583-z

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  • DOI: https://doi.org/10.1007/s10722-004-0583-z

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