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Oogenesis and lipids in gonad and liver of daubed shanny (Leptoclinus maculatus) females from Svalbard waters

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Abstract

Oocyte and liver histomorphology of the daubed shanny (Leptoclinus maculatus) from Isfjorden and Kongsfjorden in Svalbard were investigated during three Arctic seasons: summer (July), autumn (October) and winter (April). Three oocyte developmental phases were observed: primary growth phase, secondary growth phase and maturation phase. We observed four different developmental stages: (1) perinucleolus stage with cortical alveolus formation, (2) lipid droplets formation, (3) vitellogenesis stage and (4) maturation. Late maturation stage of oocytes in the ovaries was from the autumn season. Females accumulated lipids in liver (up to 35.2 % dw) and deposited large amounts of lipids into gonads (up to 52.2 % dw) during maturation. Lipid classes in female gonads changed seasonally, with relative increase in cholesterol during summer and depletion of storage lipids (triacylglycerols and wax esters/cholesterol esters) during the winter. Lipid composition in liver changed during oocyte development and spawning, as neutral lipids were transferred to developing oocytes during summer to autumn. During winter, storage lipids were depleted during starvation. Based on the increase in gonadosomatic index (GSI) with length and observed maturation stages, females seem to mature at a length of about 125–130 mm. The GSI and hepatosomatic index of large females sampled in autumn (September–October) were significantly higher than for females in late winter (April) and spring (May). These results indicate that spawning takes place during winter in Isfjorden and that energy reserves stored in the liver are utilized by females during gonadal development and reproduction.

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Acknowledgments

We are grateful to Ekaterina V. Mikodina, Marina A. Sedova and Efim Pavlov at the Russian Federal Research Institute of Fisheries and Oceanography, Moscow, for their invaluable help in organization of histology analysis in the Fish physiology and morphology lab and practical skills with histological samples and equipment. We would like to thank crew of R/V Jan Mayen (University of Tromsø, Norway) for assistance in the collection of the samples during trawling. We also acknowledge the TUNU-MAFIG Programme (University of Tromsø) for our participation and thus opportunity for sampling of material on their ‘TUNU III cruise 2007. This work was carried out as a part of the “Ice Edge Programme”, which was funded by Statoil through the Statoil-ARCTOS Arctic Research Programme (SAARP). The work was supported by the University Centre in Svalbard, Norwegian Polar Institute, and Scottish Association for Marine Science, The President Grant for Young Scientists MK-666.2011.4 and President Program “Leader Scientific schools” NSh-3731.2010.4 and NSh-1642.2012.4 (Russian Federation).

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  1. Institute of Biology of Karelian Research Centre Russian Academy of Sciences, Pushkinskaya Street, 11, 185910, Petrozavodsk, Russia

    Svetlana A. Murzina & Nina N. Nemova

  2. Norwegian Polar Institute, Fram Centre, 9296, Tromsø, Norway

    Camilla A. Meyer Ottesen, Stig Falk-Petersen & Haakon Hop

  3. Russian Federal Research Institute of Fisheries and Oceanography (VNIRO), 107140, Moscow, Russia

    Oksana G. Poluektova

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  1. Svetlana A. Murzina
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  2. Camilla A. Meyer Ottesen
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Correspondence to Svetlana A. Murzina.

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Murzina, S.A., Meyer Ottesen, C.A., Falk-Petersen, S. et al. Oogenesis and lipids in gonad and liver of daubed shanny (Leptoclinus maculatus) females from Svalbard waters. Fish Physiol Biochem 38, 1393–1407 (2012). https://doi.org/10.1007/s10695-012-9627-z

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