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Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus

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Abstract

Pine wilt disease, caused by the nematode Bursaphelenchus xylophilus, is responsible for devastation of pine forests worldwide. Until now, there are no effective ways of dealing with this serious threat. The use of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (encoded by the acdS gene)-producing plant growth-promoting bacteria has been shown to be a useful strategy to reduce the damage due to biotic and abiotic stresses. Pinus pinaster seedlings inoculated with the ACC deaminase-producing bacterium Pseudomonas putida strain UW4 showed an increased root and shoot development and reduction of B. xylophilus induced symptoms. In contrast, a P. putida UW4 acdS mutant was unable to promote pine seedling growth or to decrease B. xylophilus induced symptoms. This is the first report on the use of ACC deaminase-producing bacteria as a potential biological control agent for a tree disease, thus suggesting that the inoculation of pine seedlings grown in a tree nursery might constitute a novel strategy to obtain B. xylophilus resistant pine trees.

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Acknowledgments

This work was supported by the European Project REPHRAME—development of improved methods for detection, control and eradication of pine wood nematode in support of EU Plant Health Policy, European Union Seventh Framework Programme FP7-KBBE-2010-4 and the European Project BioReGent—Biocontrol and Bioremediation Agents and their role in Agriculture and Forest Health, European Union Seventh Framework Programme FP7-PEOPLE-2009-IRSES (grant agreement no. 247669); and FEDER Funds through the Operational Programme for Competitiveness Factors—COMPETE and National Funds through FCT—Foundation for Science and Technology under the Strategic Project PEst-C/AGR/UI0115/2011.

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Authors and Affiliations

  1. NemaLab, ICAAM—Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Núcleo da Mitra, Apartado 94, 7002-554, Évora, Portugal

    Francisco X. Nascimento, Cláudia S. L. Vicente, Pedro Barbosa, Margarida Espada & Manuel Mota

  2. Laboratório de Microbiologia do Solo, Departamento de Biologia, ICAAM—Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Núcleo da Mitra, Apartado 94, 7002-554, Évora, Portugal

    Solange Oliveira

  3. Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada

    Bernard R. Glick

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  1. Francisco X. Nascimento
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  2. Cláudia S. L. Vicente
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  3. Pedro Barbosa
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  4. Margarida Espada
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  5. Bernard R. Glick
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Correspondence to Solange Oliveira.

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Nascimento, F.X., Vicente, C.S.L., Barbosa, P. et al. Evidence for the involvement of ACC deaminase from Pseudomonas putida UW4 in the biocontrol of pine wilt disease caused by Bursaphelenchus xylophilus . BioControl 58, 427–433 (2013). https://doi.org/10.1007/s10526-012-9500-0

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