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Electrochemical and spectral behaviour of miR-34a-5p

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Abstract

MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA→miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3–6.

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Acknowledgments

This research was supported by (a) the CEITEC—Central European Institute of Technology Project CZ.1.05/1.1.00/02.0068, (b) SIX CZ.1.05/2.1.00/03.0072 and (c) KONTAKT II (LH 13053) projects of the Ministry of Education, Youth and Sports of the Czech Republic.

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Authors and Affiliations

  1. Faculty of Science, Department of Chemistry, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic

    Aneta Vecerova, Iveta Pilarova & Libuse Trnkova

  2. Faculty of Medicine, Department of Pathological Physiology, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic

    Kristyna Hudcova & Michal Masarik

  3. Central European Institute of Technology-CEITEC, Brno University of Technology, Technická 3058/10, 616 00, Brno, Czech Republic

    Libuse Trnkova

Authors
  1. Aneta Vecerova
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  2. Kristyna Hudcova
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  3. Iveta Pilarova
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  4. Michal Masarik
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  5. Libuse Trnkova
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Correspondence to Libuse Trnkova.

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Vecerova, A., Hudcova, K., Pilarova, I. et al. Electrochemical and spectral behaviour of miR-34a-5p. Monatsh Chem 147, 105–110 (2016). https://doi.org/10.1007/s00706-015-1600-y

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  • DOI: https://doi.org/10.1007/s00706-015-1600-y

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