Bioprocess and Biosystems Engineering

, Volume 30, Issue 1, pp 47–59

Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates

  • Tobias Fürch
  • Rajan Hollmann
  • Christoph Wittmann
  • Wei Wang
  • Wolf-Dieter Deckwer
Original Paper

DOI: 10.1007/s00449-006-0095-7

Cite this article as:
Fürch, T., Hollmann, R., Wittmann, C. et al. Bioprocess Biosyst Eng (2007) 30: 47. doi:10.1007/s00449-006-0095-7

Abstract

Fluxes of central carbon metabolism [glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA cycle), biomass formation] were determined for several Bacillus megaterium strains (DSM319, WH320, WH323, MS941) in C- and N-limited chemostat cultures by 13C labelling experiments. The labelling patterns of proteinogenic amino acids were analysed by GC/MS and therefrom flux ratios at important nodes within the metabolic network could be calculated. On the basis of a stoichiometric metabolic model flux distributions were estimated for the different B. megaterium strains used at various cultivation conditions. Generally all strains exhibited similar metabolic flux distributions, however, several significant changes were found in (1) the glucose flux entering the PPP via the oxidative branch, (2) the reversibilities within the PPP, (3) the relative fluxes of pyruvate and acetyl-CoA fed to the TCA cycle, (4) the fluxes around the pyruvate node involving a futile cycle.

Keywords

Bacillus megaterium Metabolic flux analysis Central carbon pathways Futile cycle 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Tobias Fürch
    • 1
  • Rajan Hollmann
    • 1
  • Christoph Wittmann
    • 2
  • Wei Wang
    • 1
  • Wolf-Dieter Deckwer
    • 1
  1. 1.Institute of Biochemical Engineering (TU-BCE)Technical University Braunschweig/HZI-Helmholtz Zentrum für InfektionsforschungBraunschweigGermany
  2. 2.Institute of Biochemical EngineeringSaarland UniversitySaarbruckenGermany

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