Summary
By developing an efficient transformation system it was possible to reintroduce two different glucose dehydrogenase genes of Bacillus megaterium into this host. These genes were previously cloned, sequenced and expressed in Escherichia coli. Since the expression of one of these genes (gdhA) turned out to be extremely high in B. megaterium, an expression system for genes from closely and distantly related organisms using the controlling region of the gdhA gene was developed.
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Dedicated to Professor Dr. Dr. h. c. K. Esser on the occasion of his 65th birthday
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Meinhardt, F., Stahl, U. & Ebeling, W. Highly efficient expression of homologous and heterologous genes in Bacillus megaterium . Appl Microbiol Biotechnol 30, 343–350 (1989). https://doi.org/10.1007/BF00296622
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DOI: https://doi.org/10.1007/BF00296622