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Ovicidal, larvicidal and adulticidal properties of Asparagus racemosus (Willd.) (Family: Asparagaceae) root extracts against filariasis (Culex quinquefasciatus), dengue (Aedes aegypti) and malaria (Anopheles stephensi) vector mosquitoes (Diptera: Culicidae)

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Abstract

Several diseases are associated to the mosquito–human interaction. Mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, West Nile virus and yellow fever. These diseases produce significant morbidity and mortality in humans and livestock around the world. The present investigation was undertaken to study the ovicidal, larvicidal and adulticidal activities of crude hexane, ethyl acetate, benzene, chloroform and methanol extracts of root of Asparagus racemosus were assayed for their toxicity against three important vector mosquitoes, viz., Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). The mean percent hatchability of the eggs was observed after 48 h post-treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. All the five solvent extracts showed moderate ovicidal activity; however, the methanol extract showed the highest ovicidal activity. The methanol extract of Asparagus racemosus against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi exerted 100 % mortality (zero hatchability) at 375, 300 and 225 ppm, respectively. Control eggs showed 99–100 % hatchability. The larval mortality was observed after 24 h of exposure. All extracts showed moderate larvicidal effects; however, the highest larval mortality was found in methanol extract of root of Asparagus racemosus against the larvae of Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi with the LC50 and LC90 values were 115.13, 97.71 and 90.97 ppm and 210.96, 179.92, and 168.82 ppm, respectively. The adult mortality was observed after 24 h recovery period. The plant crude extracts showed dose-dependent mortality. At higher concentrations, the adult showed restless movement for some times with abnormal wagging and then died. Among the extracts tested, the highest adulticidal activity was observed in methanol extract against Anopheles stephensi followed by Aedes aegypti and Culex quinquefasciatus with the LD50 and LD90 values were 120.44, 135.60, and 157.71 ppm and 214.65, 248.35, and 290.95 ppm, respectively. No mortality was recorded in the control. The finding of the present investigation revealed that the root extract of Asparagus racemosus possess remarkable ovicidal, larvicidal and adulticidal activity against medically important vector mosquitoes and this is the low cost and ideal eco-friendly approach for the control of mosquitoes. This is the first report on the mosquito ovicidal, larvicidal and adulticidal activities of the reported Asparagus racemosus root.

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References

  • Al-Doghairi M, El-Nadi A, Elhag E, Al-Ayedh H (2004) Effect of Solenostemma argel on oviposition, egg hatchability and viability of Culex pipiens L. larvae. Phytother Res 18(4):335–338

    Article  PubMed  Google Scholar 

  • Amerasan D, Murugan K, Kovendan K, Mahesh Kumar P, Panneerselvam C, Subramaniam J, John William S, Hwang J (2012) Adulticidal and repellent properties of Cassia tora Linn. (Family: Caesalpinaceae) against Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi. Parasitol Res 111:1953–1964

    Article  PubMed  Google Scholar 

  • Ansari MA, Mittal PK, Razdan RK, Sreehari U (2005) Larvicidal and mosquito repellent activities of pine (Pinus longifolia, Family: Pinaceae) oil. J Vector Borne Dis 42:95–99

    CAS  PubMed  Google Scholar 

  • Bagavan A, Kamaraj C, Abdul Rahuman A, Elango G, Abduz Zahir A, Pandiyan G (2008) Evaluation of larvicidal and nymphicidal potential of plant extracts against Anopheles subpictus Grassi, Culex tritaeniorhynchus Giles and Aphis gossypii Glover. Parasitol Res 104(5):1109–1117

    Article  PubMed  Google Scholar 

  • Berenbaum MR (1985) Brementoun revisited: allochemical interactions among in plants. Recent Adv Phytochem 19:139–169

    CAS  Google Scholar 

  • Bowers WS, Sener B, Evans PH, Bingol F, Erdogan I (1995) Activity of Turkish medicinal plants against mosquitoes Aedes aegypti and Anopheles gambiae. Inst Sci Appl 16(3/4):339–342

    Google Scholar 

  • Chen W, Isman MS, Chiu SF (1995) Antifeedant and growth inhibitory effects of the limonoid toosendanin and Media toosendan extracts on the variegated cutworm Peridroma saucia (Lep., Noctuidae). J Appl Entomol 119:367–370

    Article  Google Scholar 

  • Choochote W, Tuetun B, Kanjanapothi D, Rattanachanpichai E, Chaithong U, Chaiwong P, Jitpakdi A, Tippawangkosol P, Riyong D, Pitasawat B (2004) Potential of crude seed extract of celery, Apium graveolens L., against the mosquito Aedes aegypti (L.) (Diptera: Culicidae). J Vector Ecol 29:340–349

    PubMed  Google Scholar 

  • Corbel V, Duchon S, Zalm M, Hougard JM (2004) Dinotefuran: a potential neonicotinoid insecticide against resistant mosquitoes. J Med Entomol 41:712–717

    Article  CAS  PubMed  Google Scholar 

  • Crobsy DG (1971) Minor insecticides of plant origin. In: Jacobson M, Crobsy DG (eds) Naturally occurring insecticides. Marcel Dekker, New York, pp 171–239

    Google Scholar 

  • Dua VK, Alam MF, Pandey AC, Rai S, Chopra AK, Kaul VK (2008) Insecticidal activity of Valeriana jatamansi (Valerianaceae) against mosquitoes. J Am Mosq Control Assoc 24:315–318

    Article  CAS  PubMed  Google Scholar 

  • Dua VK, Pandey AC, Dash AP (2010) Adulticidal activity of essential oil of Lantana camara leaves against mosquitoes. Indian J Med Res 131:434–439

    CAS  PubMed  Google Scholar 

  • Elango G, Rahuman AA, Bagavan A, Kamaraj C, Zahir AA, Venkatesan C (2009) Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus. Parasitol Res 104(6):1381–1388

    Article  CAS  PubMed  Google Scholar 

  • Elango G, Abdul Rahuman A, Kamaraj C, Bagavan A, Abduz Zahir A (2012) Adult emergence inhibition and adulticidal activity of leaf crude extracts against Japanese encephalitis vector, Culex tritaeniorhynchus. J King Saud Univ Science 24:73–80

    Article  Google Scholar 

  • EL-Sheikh TMY, Hassan MI, Moselhy WA, Amer MS, Shehata AZ (2011) Evaluation of the biological activity of some Cupressus semprevirens (Cupressaceae) extracts against the mosquito vector Culex pipiens L. (Diptera: Culicidae). Egypt Acad J Biol Sci 4(1):33–48

    Google Scholar 

  • Finney DJ (1971) Probit analysis. Cambridge University Press, London, pp 68–78

    Google Scholar 

  • Fradin MS, Day JF (2002) Comparative efficacy of insect repellents against mosquitoes bites. N Engl J Med 347:13–18

    Article  CAS  PubMed  Google Scholar 

  • Georges K, Jayaprakasam B, Dalavoy SS, Nair MG (2008) Pest managing activities of plant extracts and anthraquinones from Cassia nigricans from Burkina Faso. Bioresour Technol 99(6):2037–2045

    Article  CAS  PubMed  Google Scholar 

  • Govindarajan M (2009) Bioefficacy of Cassia fistula Linn. (Leguminosae) leaf extract against chikungunya vector, Aedes aegypti (Diptera: Culicidae). Eur Rev Med Pharmacol Sci 13(2):99–103

    CAS  PubMed  Google Scholar 

  • Govindarajan M (2010) Larvicidal and repellent activities of Sida acuta Burm. F. (Family: Malvaceae) against three important vector mosquitoes. Asian Pac J Trop Med 3:691–695

    Article  Google Scholar 

  • Govindarajan M (2011a) Evaluation of indigenous plant extracts against the malarial vector, Anopheles stephensi (Liston) (Diptera: Culicidae). Parasitol Res 109:93–103

    Article  PubMed  Google Scholar 

  • Govindarajan M (2011b) Mosquito larvicidal and ovicidal activity of Cardiospermum halicacabum Linn. (Family: Sapindaceae) leaf extract against Culex quinquefasciatus (Say.) and Aedes aegypti (Linn.) (Diptera: Culicidae). Eur Rev Med Pharmacol Sci 15(7):787–794

    CAS  PubMed  Google Scholar 

  • Govindarajan M, Karuppannan P (2011) Mosquito larvicidal and ovicidal properties of Eclipta alba (L.) Hassk (Asteraceae) against chikungunya vector, Aedes aegypti (Linn.) (Diptera: Culicidae). Asian Pac J Trop Med 4(1):24–28

    Article  CAS  PubMed  Google Scholar 

  • Govindarajan M, Sivakumar R (2012a) Adulticidal and repellent properties of indigenous plant extracts against Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). Parasitol Res 110:1607–1620

    Article  PubMed  Google Scholar 

  • Govindarajan M, Sivakumar R (2012b) Adulticidal properties of Cardiospermum halicacabum plant extracts against three important vector mosquitoes. Eur Rev Med Pharmacol Sci 16(3):95–104

    PubMed  Google Scholar 

  • Govindarajan M, Jebanesan A, Reetha D, Amsath R, Pushpanathan T, Samidurai K (2008) Antibacterial activity of Acalypha indica L. Eur Rev Med Pharmacol Sci 12(5):299–302

    CAS  PubMed  Google Scholar 

  • Govindarajan M, Mathivanan T, Elumalai K, Krishnappa K, Anandan A (2011) Ovicidal and repellent activities of botanical extracts against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). Asian Pac J Trop Biomed 1(1):43–48

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hafeez F, Akram W, Suhail A, Aslam Khan M (2010) Adulticidal action of ten citrus oils against Aedes albopictus (Diptera: Culicidae). Pak J Agric Sci 47(3):241–244

    Google Scholar 

  • Halim ASA (2008) Efficacy of Zingiber officinale on third stage larvae and adult fecundity of Musca domestica and Anopheles pharoensis. J Egypt Soc Parasitol 38:385–392

    PubMed  Google Scholar 

  • Harve G, Kamath V (2004) Larvicidal activity of plant extracts used alone and in combination with known synthetic larvicidal agents against Aedes aegypti. Indian J Exp Biol 42(12):1216–1219

    PubMed  Google Scholar 

  • Jang YS, Baek BR, Yang YC, Kim MK, Lee HS (2002) Larvicidal activity of leguminous seeds and grains against Aedes aegypti and Culex pipiens pallens. J Am Mosq Control Assoc 18(3):210–213

    PubMed  Google Scholar 

  • Jeyabalan D, Arul N, Thangamathi P (2003) Studies on effects of Pelargonium citrosa leaf extracts on malarial vector, Anopheles stephensi Liston. Bioresour Technol 89:185–189

    Article  CAS  PubMed  Google Scholar 

  • Kalyanasundaram M, Das PK (1985) Larvicidal and synergistic activity of plant extracts for mosquito control. Indian J Med Res 82:19–23

    CAS  PubMed  Google Scholar 

  • Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2008) Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171

    Article  PubMed  Google Scholar 

  • Kannathasan K, Senthilkumar A, Chandrasekaran M, Venkatesalu V (2007) Differential larvicidal efficacy of four species of Vitex against Culex quinquefasciatus larvae. Parasitol Res 101(6):1721–1723

    Article  PubMed  Google Scholar 

  • Komalamisra N, Trongtokit Y, Rongsriyam Y, Apiwathnasorn C (2005) Screening for larvicidal activity in some Thai plants against four mosquito vector species. Southeast Asian J Trop Med Public Health 36(6):1412–1422

    PubMed  Google Scholar 

  • Kovendan K, Murugan K, Vincent S, Kamalakannan S (2011) Larvicidal efficacy of Jatropha curcas and bacterial insecticide, Bacillus thuringiensis, against lymphatic filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 109:1251–1257

    Article  PubMed  Google Scholar 

  • Kuppusamy C, Murugan K (2006) Mosquitocidal effect of ethanolic extracts of Andrographis paniculata Nees on filarial vector Culex quinquefasciatus Say (Diptera: Culicidae). In: International Conference on Diversity of Insects: Challenging Issues in Management and Conservation, 30 January–3 February 2006, Tamil Nadu, India pp. 194

  • Maheswaran R, Sathish S, Ignacimuthu S (2008) Larvicidal activity of Leucas aspera (Wild.) against the larvae of Culex quinquefasciatus Say. and Aedes aegypti L. Int J Integr Biol 2:214–217

    Google Scholar 

  • Malarvannan S, Giridharan R, Sekar S, Prabavathy VR, Nair S (2009) Ovicidal activity of crude extracts of few traditional plants against Helicoverpa armigera (Hubner) (Noctuidae: Lepidoptera). J Biopest 2:64–71

    CAS  Google Scholar 

  • Manimegalai K, Annapoorani CA, Dhanalakshmi D (2011) Evaluation of larvicidal activity of the leaf and seed extracts of Abrus precatorius against Culex quinquefasciatus (Diptera: Culicidae). Plant Arch 11(1):311–313

    Google Scholar 

  • Mansour SA, Messeha SS, El-Gengaihi SE (2000) Botanical biocides. 4. Mosquitocidal activity of certain Thymus capitatus constituents. J Nat Toxins 9(1):49–62

    CAS  PubMed  Google Scholar 

  • Marimuthu S, Rahuman AA, Govindasamy R, Thirunavukkarasu S, Arivarasan VK, Chidambaram J, Asokan B, Zahir AA, Elango G, Chinnaperumal K (2011) Evaluation of green synthesized silver nanoparticles against parasites. Parasitol Res 108(6):1541–1549

    Article  PubMed  Google Scholar 

  • Mohan L, Sharma P, Srivastava CN (2007) Comparative efficacy of Solanum xanthocarpum extracts alone and in combination with a synthetic pyrethroid, cypermethrin, against malaria vector, Anopheles stephensi. Southeast Asian J Trop Med Public Health 38(2):256–260

    PubMed  Google Scholar 

  • Mullai K, Jebanesan A (2006) Larvicidal and ovicidal activity of the leaf extract of two cucurbitaceous plants against filarial vector, Culex quinquefasciatus Say. Ind J Environ Ecoplan 12:611–615

    CAS  Google Scholar 

  • Mullai K, Jebanesan A, Pushpanathan T (2008) Effect of bioactive fractions of Citrullus vulgaris Schrad. leaf extract against Anopheles stephensi and Aedes aegypti. Parasitol Res 102(5):951–955

    Article  CAS  PubMed  Google Scholar 

  • Murugan K, Mahesh Kumar P, Kovendan K, Amerasan D, Subrmaniam J, Shiou HJ (2012) Larvicidal, pupicidal, repellent and adulticidal activity of Citrus sinensis orange peel extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 111:1757–1769

    Article  PubMed  Google Scholar 

  • Nadkarni AK (1976) Indian materia medica. Pop Prakasham (Mumbai) 1(3):1292–1294

    Google Scholar 

  • Ouda NAA, Al-chalabi BBM, Al-charchafchi FFMR, Mohsen ZZH (1998) Extract of Atriplex canescens against Culex quinquefasciatus. Pharm Biol 36(1):69–71

    Article  Google Scholar 

  • Pancharoen C, Kulwichit W, Tantawichien T, Thisyakorn U, Thisyakorn C (2002) Dengue infection: a global concern. J Med Assoc Thai 85:25–33

    Google Scholar 

  • Pangnakorn U, Kanlaya S, Kuntha C (2011) Efficiency of wood vinegar and extracts from some medicinal plants on insect control. Adv Environ Biol 5(2):477–482

    Google Scholar 

  • Panneerselvam C, Murugan K (2013) Adulticidal, repellent, and ovicidal properties of indigenous plant extracts against the malarial vector, Anopheles stephensi (Diptera: Culicidae). Parasitol Res 112:679–692

    Article  PubMed  Google Scholar 

  • Prabakar K, Jebanesan A (2004) Larvicidal efficacy of some Cucurbitacious plant leaf extracts against Culex quinquefasciatus (Say). Bioresour Technol 95(1):113–114

    Article  CAS  PubMed  Google Scholar 

  • Prabhu K, Murugan K, Nareshkumar A, Ramasubramanian N, Bragadeeswaran S (2011) Larvicidal and repellent potential of Moringa oleifera against malarial vector, Anopheles stephensi Liston (Insecta: Diptera: Culicidae). Asian Pac J Trop Biomed 1:127–132

    Article  Google Scholar 

  • Priyanka, Prakash S (2003) Laboratory efficacy tests for fungal metabolites of Chrysosporium tropicum against Culex quinquefasciatus. J Am Mosq Control Assoc 19(4):404–407

    CAS  PubMed  Google Scholar 

  • Pushpanathan T, Jebanesan A, Govindarajan M (2006) Larvicidal, ovicidal and repellent activities of Cymbopogan citrates Stapf (Graminae) essential oil against the filarial mosquito Culex quinquefasciatus (Say) (Diptera: Culicidae). Trop Biomed 23(2):208–212

    CAS  PubMed  Google Scholar 

  • Rahuman AA, Venkatesan P (2008) Larvicidal efficacy of five cucurbitaceous plant leaf extracts against mosquito species. Parasitol Res 103:133–139

    Article  PubMed  Google Scholar 

  • Rajasekariah GR, Parab PB, Chandrashekar R, Deshpande L, Subrahmanyam D (1991) Pattern of Wuchereria bancrofti microfilaraemia in young and adolescent school children in Bassein, India, an endemic area for lymphatic filariasis. Ann Trop Med Parasitol 85(6):663–665

    CAS  PubMed  Google Scholar 

  • Rajkumar S, Jebanesan A (2004) Mosquitocidal activities of octasane from Moschosma polystachyum Linn. (Lamiaceae). J Ethnopharmacol 90:87–89

    Article  CAS  PubMed  Google Scholar 

  • Rajkumar S, Jebanesan (2005) Oviposition deterrent and skin repellent activities of Solanum trilobatum leaf extract against the malarial vector Anopheles stephensi. J Insect Sci 5:15

    CAS  PubMed Central  PubMed  Google Scholar 

  • Rohani A, Nazni WA, Ngo LV, Ibrahim J, Lee HL (1997) Adulticidal properties of the essential extracts of some Malaysian plants on vector mosquitoes. Trop Biomed 14:5–9

    Google Scholar 

  • Saravanan KS, Periyanayagam K, Ismail M (2007) Mosquito larvicidal properties of various extract of leaves and fixed oil from the seeds of Caesalpinia bonduc (L) Roxb. J Commun Dis 39(3):153–157

    PubMed  Google Scholar 

  • Senthilkumar N, Varma P, Gurusubramanian G (2009) Larvicidal and adulticidal activities of some medicinal plants against the malarial vector Anopheles stephensi (Liston). Parasitol Res 104:237–244

    Article  CAS  PubMed  Google Scholar 

  • Shaalan EAS, Canyonb D, Younesc MWF, Abdel-Wahaba H, Mansoura AH (2005) A review of botanical phytochemicals with mosquitocidal potential. Environ Int 31:1149–1166

    Article  CAS  PubMed  Google Scholar 

  • Sharma P, Mohan L, Srivastava CN (2005) Larvicidal potential of Nerium indicum and Thuja oriertelis extracts against malaria and Japanese encephalitis vector. J Environ Biol 26(4):657–660

    PubMed  Google Scholar 

  • Su T, Mulla MS (1998) Ovicidal activity of neem products (Azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J Am Mosq Control Assoc 14:204–209

    CAS  PubMed  Google Scholar 

  • Sulaiman S, Kadir AA, Pawanchee ZA, Othman HF, Shaari N, Wahab A, Rahman ARA, Sohadi AR (2001) Field evaluation of the control efficacy of plant extracts applied by ULV spraying at high-rise flats to control dengue vectors. Arbovirus Res Aust 8:375–378

    Google Scholar 

  • World Health Organisation (2010) Malaria Factsheet No.94. World Health Organisation, Geneva

    Google Scholar 

  • World Health Organization (1981) Instruction for determining the susceptibility or resistance of adult mosquitoes to organochlorine, organophosphate and carbamate insecticides. WHO/VBC/81.806

  • World Health Organization (2005) Guidelines for laboratory and field testing of mosquito larvicides. Communicable disease control, prevention and eradication, WHO pesticide evaluation scheme. WHO, Geneva, WHO/CDS/WHOPES/GCDPP/1.3

  • Xiao XM, Hu ZN, Shi BJ, Wei SP, Wu WJ (2012) Larvicidal activity of lignans from Phryma leptostachya L. against Culex pipiens pallens. Parasitol Res 110:1079–1084

    Article  PubMed  Google Scholar 

  • Yadav R, Srivastava VK, Chandra R, Singh A (2002) Larvicidal activity of latex and stem bark of Euphorbia tirucalli plant on the mosquito Culex quinquefasciatus. J Commun Dis 34(4):264–269

    PubMed  Google Scholar 

  • Zahir AA, Rahuman AA, Bagavan A, Santhoshkumar T, Mohamed RR, Kamaraj C, Rajakumar G, Elango G, Jayaseelan C, Marimuthu S (2010) Evaluation of botanical extracts against Haemaphysalis bispinosa Neumann and Hippobosca maculate Leach. Parasitol Res 107:585–592

    Article  PubMed  Google Scholar 

  • Zaridah MZ, Idid SZ, Omar AW, Khozirah S (2001) In vitro antifilarial effects of three plant species against adult worms of subperiodic Brugia malayi. J Ethnopharmacol 78(1):79–84

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to Indian Council of Medical Research (ICMR Ref. Letter No. 5/8-7 (246)/2012-ECD-II), New Delhi, India, for providing financial assistance and the authors would like to thank Dr. N. Indra, Professor and Head of the Department of Zoology, Annamalai University, for the laboratory facilities provided. The authors would also like to acknowledge the cooperation of staff members of the VCRC (ICMR), Pondicherry.

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  1. Unit of Vector Biology and Phytochemistry, Department of Zoology, Annamalai University, Annamalai Nagar, 608 002, Tamil Nadu, India

    Marimuthu Govindarajan & Rajamohan Sivakumar

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Govindarajan, M., Sivakumar, R. Ovicidal, larvicidal and adulticidal properties of Asparagus racemosus (Willd.) (Family: Asparagaceae) root extracts against filariasis (Culex quinquefasciatus), dengue (Aedes aegypti) and malaria (Anopheles stephensi) vector mosquitoes (Diptera: Culicidae). Parasitol Res 113, 1435–1449 (2014). https://doi.org/10.1007/s00436-014-3784-1

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