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Anti-microbial and cytotoxic 1,6-dihydroxyphenazine-5,10-dioxide (iodinin) produced by Streptosporangium sp. DSM 45942 isolated from the fjord sediment

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Abstract

Phenazine natural products/compounds possess a range of biological activities, including anti-microbial and cytotoxic, making them valuable starting materials for drug development in several therapeutic areas. These compounds are biosynthesized almost exclusively by eubacteria of both terrestrial and marine origins from erythrose 4-phosphate and phosphoenol pyruvate via the shikimate pathway. In this paper, we report isolation of actinomycete bacteria from marine sediment collected in the Trondheimfjord, Norway. Screening of the isolates for biological activity produced several “hits”, one of which was followed up by identification and purification of the active compound from the actinomycete bacterium Streptosporangium sp. The purified compound, identified as 1,6-dihydroxyphenazine-5,10-dioxide (iodinin), was subjected to extended tests for biological activity against bacteria, fungi and mammalian cells. In these tests, the iodinin demonstrated high anti-microbial and cytotoxic activity, and was particularly potent against leukaemia cell lines. This is the first report on the isolation of iodinin from a marine-derived Streptosporangium.

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Acknowledgements

We thank I.F. Nes for providing Enterococcus faecium CTC 492 for bioactivity testing. This study was supported by the Research Council of Norway, University of Bergen, Norwegian University of Science and Technology, and SINTEF.

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Authors and Affiliations

  1. SINTEF Materials and Chemistry, Department of Biotechnology, Sem Sælands vei 2a, 7465, Trondheim, Norway

    Håvard Sletta, Kristin F. Degnes, Geir Klinkenberg, Kolbjørn Zahlsen, Anders Brunsvik & Trond E. Ellingsen

  2. Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5009, Bergen, Norway

    Lars Herfindal & Stein O. Døskeland

  3. Department of Biotechnology, Norwegian University of Science and Technology, Sem Sælans vei 6/8, 7495, Trondhiem, Norway

    Espen Fjærvik, Gyrid Nygaard, Finn L. Aachmann & Sergey B. Zotchev

Authors
  1. Håvard Sletta
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  2. Kristin F. Degnes
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  3. Lars Herfindal
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  4. Geir Klinkenberg
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  5. Espen Fjærvik
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  6. Kolbjørn Zahlsen
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  7. Anders Brunsvik
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  11. Stein O. Døskeland
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  12. Sergey B. Zotchev
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Correspondence to Sergey B. Zotchev.

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Sletta, H., Degnes, K.F., Herfindal, L. et al. Anti-microbial and cytotoxic 1,6-dihydroxyphenazine-5,10-dioxide (iodinin) produced by Streptosporangium sp. DSM 45942 isolated from the fjord sediment. Appl Microbiol Biotechnol 98, 603–610 (2014). https://doi.org/10.1007/s00253-013-5320-0

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  • DOI: https://doi.org/10.1007/s00253-013-5320-0

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