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Evaluation of novel lactose-positive and exopolysaccharide-producing strain of Pediococcus pentosaceus for fermented foods

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Abstract

A novel strain of lactic acid bacteria Pediococcus pentosaceus P 773 was isolated from spoiled beer and identified by means of 16S rDNA sequence analysis. The ability to assimilate lactose as a sole carbon source as a specific feature for this strain was detected and confirmed on dairy substrates. In the presence of sucrose containing substrates (sucrose, raffinose) this P. pentosaceus P 773 lactose-positive strain produced a complex of extracellular polysaccharides (Qp = 0.08 g/l/h) with a molecular mass about 2,000 kDa composed by glucose and fructose residues at a ratio 3:1, respectively. These exopolysaccharides were capable to stimulate the growth rate and biomass productivity of common constituent cultures of probiotic dairy starters (Bifidobacterium lactis, Lactobacillus acidophilus, Streptococcus thermophilus) as well as were assimilated as a sole carbon source by these strains. The present study confirmed the presence of lactose-positive and exopolysaccharide-producing strain of P. pentosaceus in natural environment which could be used as a starter culture to impart more functional attributes to fermented food.

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References

  1. Leroy F, De Vuyst L (2004) Trends Food Sci Technol 15:67–78

    Article  CAS  Google Scholar 

  2. Russ-Madiedo P, De los Reyes-Cavilan CG (2005) J Dairy Sci 88:843–856

    Google Scholar 

  3. In LJ, Jiang ST (2001) J Food Sci 66:742–746

    Article  Google Scholar 

  4. Luchansky JB, Glass KA, Harsono KD, Degnan AJ, Faith NG, Cauvin B, Baccus-Taylor G, Arihara K, Bater B, Maurer AJ (1992) Appl Environ Microbiol 58:3053–3059

    CAS  Google Scholar 

  5. Spicka J, Kalac P, Bover-Cid S, Krizek M (2002) Eur Food Res Technol 215:509–514

    Article  CAS  Google Scholar 

  6. Kleinschmit DH, Kung L (2006) J Dairy Sci 89:3999–4004

    Article  CAS  Google Scholar 

  7. Roccach M, Tully T (1999) Food Sci Tech Int 5:243–249

    Article  Google Scholar 

  8. Simpson WJ, Taguchi H (1995) The genus Pediococcus, with notes on the genera Tetratogenococcus and Aerococcus. In: Wood BJB, Holzapfel WH (eds) The genera of Lactic acid Bacteria. Chapman & Hall, London, pp 125–172

    Google Scholar 

  9. Smitinont T, Tanasakul C, Tanasupawat S, Keeratipibul S, Navarini L, Bosco M, Cescutti P (1999) Int J Food Microbiol 51:105–111

    Article  CAS  Google Scholar 

  10. Caldvell SL, McMahon DJ, Oberg GJ, Broadbent JR (1996) Appl Environ Microbiol 62:936–941

    Google Scholar 

  11. Garvie EI (1986) Genus Pediococcus. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 2, 9th edn. The Williams & Wilkins, Baltimore, pp 1075–1079

    Google Scholar 

  12. Bengmark S, Gil A (2006) Nutr Hosp 21:72–84

    CAS  Google Scholar 

  13. Brink M, Fraser T, Todorov SD, Vaz-Velho M, Senekal M, Dicks LMT (2003) Electron J Environ Agric Food Chem 2:504–509

    Google Scholar 

  14. Gardiner GE, Casey PG, Casey G, Casey G, Lynch PB, Lawlor PG, Hill C, Fitzgerald GF, Stanton C, Ross P (2004) Appl Environ Microbiol 70:1895–1906

    Article  CAS  Google Scholar 

  15. Bhowmik T, Marth EH (1990) J Dairy Sci 73:859–866

    CAS  Google Scholar 

  16. Bekers M, Marauska M, Laukevics J, Grube M, Vigants A, Karklina D, Skudra L, Viesturs U (2001) Food Biotechnol 15:1–12

    Article  Google Scholar 

  17. Klaenhammer TR (1984) Adv Appl Microbiol 30:1–29

    Article  CAS  Google Scholar 

  18. Riebel WJ, Washington JA (1990) J Clin Microbiol 28:1348–1355

    CAS  Google Scholar 

  19. Collins MD, Williams AM, Wallbanks S (1990) FEMS Microbiol Lett 58:255–262

    Article  CAS  Google Scholar 

  20. Welman AD, Maddox IS (2003) Trends Biotechnol 21:269–274

    Article  CAS  Google Scholar 

  21. Semjonovs P, Zikmanis P (2007) J Food Technol 5:123–130

    CAS  Google Scholar 

  22. Tieking M, Korakli M, Ehrmann M, Gaenzle MG, Vogel RF (2003) Appl Environ Microbiol 69:945–952

    Article  CAS  Google Scholar 

  23. Martensson O, Biorklund M, Lambo A, Duenas-Chasco M, Irastorza A, Holst O, Norin E, Welling G, Oste R, Onning G (2005) Nutr Res 25:429–442

    Article  CAS  Google Scholar 

  24. Dal Bello F, Walter J, Hertel C, Hammes WP (2001) Syst Appl Microbiol 24:232–237

    Article  CAS  Google Scholar 

  25. Llauberes RM, Richard B, Lonvaud A, Dubourdieu D, Fournet B (1990) Carbohydr Res 203:103–107

    Article  CAS  Google Scholar 

  26. Manca de Nadra MC, Strasser de Saad AM (1995) Int J Food Microbiol 27:101–106

    Article  Google Scholar 

  27. Di Cagno R, De Angelis M, Limitone A, Minervini F, Carnevall P, Corsetti A, Gaenzle M, Ciati R, Gobbetti M (2006) J Agric Food Chem 54:9873–9881

    Article  CAS  Google Scholar 

  28. De Man JC, Rogosa M, Sharpe ME (1960) J Appl Bacteriol 23:130–135

    Google Scholar 

  29. Andersone I, Auzina L, Vigants A, Mutere O, Zikmanis P (2004) Eng Life Sci 4:56–59

    Article  CAS  Google Scholar 

  30. Bekers M, Laukevics J, Upite D, Kaminska E, Vigants A, Viesturs U, Pankova L, Danilevics A (2002) Proc Biochem 38:701–706

    Article  CAS  Google Scholar 

  31. Morris DL (1948) Science 107:254–255

    Article  CAS  Google Scholar 

  32. Sedmak JJ, Grossberg SE (1977) Anal Biochem 79:544–552

    Article  CAS  Google Scholar 

  33. Van Geel-Schutten GH, Faber EJ, Smit E, Bonting K, Smith MR, Ten Brink B, Kamerling JP, Vliegenthart JFG, Dijkhuizen L (1999) Appl Environ Microbiol 65:3008–3014

    Google Scholar 

  34. McIntosh M, Stone BA, Stanisich VA (2005) Appl Microbiol Biotechnol 68:163–173

    Article  CAS  Google Scholar 

  35. Robyt JF, Walseth TF (1978) Carbohydr Res 61:433–445

    Article  CAS  Google Scholar 

  36. Semjonovs P, Marauska M, Linde R, Grube M, Zikmanis P, Bekers M (2004) Eng Life Sci 5:433–437

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the European Social Foundation, Project ESSS 2004/3, Contract No. 2004/0001/VPD1/ESF/PIAA/04/NP/3.2.3.1/0001/0063.

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Authors and Affiliations

  1. Institute of Microbiology and Biotechnology, University of Latvia, 4, Kronvalda boulv., Riga, 1010, Latvia

    Pavels Semjonovs & Peteris Zikmanis

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  1. Pavels Semjonovs
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  2. Peteris Zikmanis
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Correspondence to Peteris Zikmanis.

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Semjonovs, P., Zikmanis, P. Evaluation of novel lactose-positive and exopolysaccharide-producing strain of Pediococcus pentosaceus for fermented foods. Eur Food Res Technol 227, 851–856 (2008). https://doi.org/10.1007/s00217-007-0796-4

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  • DOI: https://doi.org/10.1007/s00217-007-0796-4

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