Skip to main content
SpringerLink
Log in

Fine mapping and chromosome walking towards the Ror1 locus in barley (Hordeum vulgare L.)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Key message

The Ror1 gene was fine-mapped to the pericentric region of barley chromosome 1HL.

Abstract

Recessively inherited loss-of-function alleles of the barley (Hordeum vulgare) Mildew resistance locus o (Mlo) gene confer durable broad-spectrum disease resistance against the obligate biotrophic fungal powdery mildew pathogen Blumeria graminis f.sp. hordei. Previous genetic analyses revealed two barley genes, Ror1 and Ror2, that are Required for mlo-specified resistance and basal defence. While Ror2 was cloned and shown to encode a t-SNARE protein (syntaxin), the molecular nature or Ror1 remained elusive. Ror1 was previously mapped to the centromeric region of the long arm of barley chromosome 1H. Here, we narrowed the barley Ror1 interval to 0.18 cM and initiated a chromosome walk using barley yeast artificial chromosome (YAC) clones, next-generation DNA sequencing and fluorescence in situ hybridization. Two non-overlapping YAC contigs containing Ror1 flanking genes were identified. Despite a high degree of synteny observed between barley and the sequenced genomes of the grasses rice (Oryza sativa), Brachypodium distachyon and Sorghum bicolor across the wider chromosomal area, the genes in the YAC contigs showed extensive interspecific rearrangements in orientation and order. Consequently, the position of a Ror1 homolog in these species could not be precisely predicted, nor was a barley gene co-segregating with Ror1 identified. These factors have prevented the molecular identification of the Ror1 gene for the time being.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Altschul S, Gish W, Miller W, Myers E, Lipman D et al (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  • Bednarek P, Pislewska-Bednarek M, Svatos A, Schneider B, Doubsky J, Mansurova M, Humphry M, Consonni C, Panstruga R, Sanchez-Vallet A, Molina A, Schulze-Lefert P et al (2009) A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense. Science 323:101–106

    Article  PubMed  CAS  Google Scholar 

  • Bednarek P, Piślewska-Bednarek M, van Loren Themaat E, Maddula RK, Svatoš A, Schulze-Lefert P et al (2011) Conservation and clade-specific diversification of pathogen-inducible tryptophan and indole glucosinolate metabolism in Arabidopsis thaliana relatives. New Phytol 192:713–726

    Article  PubMed  CAS  Google Scholar 

  • Bennetzen JL, Ramakrishna W (2002) Numerous small rearrangements of gene content, order and orientation differentiate grass genomes. Plant Mol Biol 48:821–827

    Article  PubMed  CAS  Google Scholar 

  • Bjørnstad Å, Demissie A, Kilian A, Kleinhofs A et al (1997) The distinctness and diversity of Ethiopian barleys. Theor Appl Genet 94:514–521

    Article  Google Scholar 

  • Bowers JE, Arias MA, Asher R, Avise JA, Ball RT, Brewer GA, Buss RW, Chen AH, Edwards TM, Estill JC, Exum HE, Goff VH, Herrick KL, Steele SLM, Karunakaran S, Lafayette GK, Lemke C, Marler BS, Masters SL, McMillan JM, Nelson LK, Newsome GA, Nwakanma CC, Odeh RN, Phelps CA, Rarick EA, Rogers CJ, Ryan SP, Slaughter KA, Soderlund CA, Tang H, Wing RA, Paterson AH et al (2005) Comparative physical mapping links conservation of microsynteny to chromosome structure and recombination in grasses. Proc Natl Acad Sci USA 102:13206–13211

    Article  PubMed  CAS  Google Scholar 

  • Brueggeman R, Rostoks N, Kudrna D, Kilian A, Han F, Chen J, Druka A, Steffenson B, Kleinhofs A et al (2002) The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. Proc Natl Acad Sci USA 99:9328–9333

    Article  PubMed  CAS  Google Scholar 

  • Brunner S, Keller B, Feuillet C (2003) A large rearrangement involving genes and low-copy DNA interrupts the microcollinearity between rice and barley at the Rph7 locus. Genetics 164:673–683

    PubMed  CAS  Google Scholar 

  • Büschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A, van Daelen R, van der Lee T, Diergaarde P, Groenendijk J, Töpsch S, Vos P, Salamini F, Schulze-Lefert P et al (1997) The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88:695–705

    Article  PubMed  Google Scholar 

  • Chaplin DD, Brownstein BH (2001a) Analysis of isolated YAC clones. Curr Protoc Mol Biol. doi:10.1002/0471142727.mb0610s20 (John Wiley & Sons, Inc., pp 6.10.11–16.10.19)

    Google Scholar 

  • Chaplin DD, Brownstein BH (2001b) Overview of strategies for screening YAC libraries and analyzing YAC clones. Curr Protoc Mol Biol. doi:10.1002/0471142727.mb0609s20 (John Wiley & Sons, Inc., pp 6.9.1–6.9.7)

    Google Scholar 

  • Chen A, Brûlé-Babel A, Baumann U, Collins NC (2009) Structure-function analysis of the barley genome: the gene-rich region of chromosome 2HL. Funct Integr Genomics 9:67–79

    Article  PubMed  CAS  Google Scholar 

  • Collins NC, Lahaye T, Peterhänsel C, Freialdenhoven A, Corbitt M, Schulze-Lefert P et al (2001) Sequence haplotypes revealed by sequence-tagged site fine mapping of the Ror1 gene in the centromeric region of barley chromosome 1H. Plant Physiol 125:1236–1247

    Article  PubMed  CAS  Google Scholar 

  • Collins NC, Thordal-Christensen H, Lipka V, Bau S, Kombrink E, Qiu J-L, Hückelhoven R, Stein M, Freialdenhoven A, Somerville SC, Schulze-Lefert P et al (2003) SNARE-protein-mediated disease resistance at the plant cell wall. Nature 425:973–977

    Article  PubMed  CAS  Google Scholar 

  • Consonni C, Humphry ME, Hartmann HA, Livaja M, Durner J, Westphal L, Vogel J, Lipka V, Kemmerling B, Schulze-Lefert P, Somerville SC, Panstruga R et al (2006) Conserved requirement for a plant host cell protein in powdery mildew pathogenesis. Nat Genet 38:716–720

    Article  PubMed  CAS  Google Scholar 

  • Consonni C, Bednarek P, Humphry M, Francocci F, Ferrari S, Harzen A, Ver Loren van Themaat E, Panstruga R et al (2010) Tryptophan-derived metabolites are required for antifungal defense in the Arabidopsis mlo2 mutant. Plant Physiol 152:1544–1561

    Article  PubMed  CAS  Google Scholar 

  • Devoto A, Piffanelli P, Nilsson I, Wallin E, Panstruga R, von Heijne G, Schulze-Lefert P et al (1999) Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem 274:34993–35004

    Article  PubMed  CAS  Google Scholar 

  • Draper J, Mur LAJ, Jenkins G, Ghosh-Biswas GC, Bablak P, Hasterok R, Routledge APM et al (2001) Brachypodium distachyon. A new model system for functional genomics in grasses. Plant Physiol 127:1539–1555

    Article  PubMed  CAS  Google Scholar 

  • Favret E (1965) Induced mutations in breeding for disease resistance. In: Nations FaAOotU (ed) The use of induced mutations in plant breeding. Pergamon Press, London, pp 521–536

    Google Scholar 

  • Foote TN, Griffiths S, Allouis S, Moore G (2004) Construction and analysis of a BAC library in the grass Brachypodium sylvaticum: its use as a tool to bridge the gap between rice and wheat in elucidating gene content. Funct Integr Genomics 4:26–33

    Article  PubMed  CAS  Google Scholar 

  • Freialdenhoven A, Peterhänsel C, Kurth J, Kreuzaler F, Schulze-Lefert P et al (1996) Identification of genes required for the function of non-race-specific mlo resistance to powdery mildew in barley. Plant Cell 8:5–14

    PubMed  CAS  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hückelhoven R, Trujillo M, Kogel K-H et al (2000) Mutations in Ror1 and Ror2 genes cause modification of hydrogen peroxide accumulation in mlo-barley under attack from the powdery mildew fungus. Mol Plant Pathol 1:287–292

    Article  PubMed  Google Scholar 

  • Humphry M, Bednarek P, Kemmerling B, Koh S, Stein M, Göbel U, Stüber K, Piślewska-Bednarek M, Loraine A, Schulze-Lefert P, Somerville S, Panstruga R et al (2010) A regulon conserved in monocot and dicot plants defines a functional module in antifungal plant immunity. Proc Natl Acad Sci USA 107:21896–21901

    Article  PubMed  CAS  Google Scholar 

  • Jarosch B, Kogel K-H, Schaffrath U (1999) The ambivalence of the barley Mlo locus: mutations conferring resistance against powdery mildew (Blumeria graminis f. sp. hordei) enhance susceptibility to the rice blast fungus Magnaporthe grisea. Mol Plant Microbe Interact 12:508–514

    Article  CAS  Google Scholar 

  • Jarosch B, Collins NC, Zellerhoff N, Schaffrath U et al (2005) RAR1, ROR1, and the actin cytoskeleton contribute to basal resistance to Magnaporthe grisea in barley. Mol Plant Microbe Interact 18:397–404

    Article  PubMed  CAS  Google Scholar 

  • Jørgensen J (1976) Identification of powdery mildew resistant barley mutants and their allelic relationship. In: Gaul H (ed) Barley genetics III: Proceedings of the third international barley genetics symposium, Verlag Karl Thiemig, Garching, 7–12 July 1975, pp 446–455

  • Jørgensen JH (1992a) Sources and genetics of resistance to fungal pathogens. In: Shewry PR (ed) Barley: genetics, biochemestry, molecular biology and biotechnology. CAB International, Wallingford, Oxfordshire, UK, pp 441–457

    Google Scholar 

  • Jørgensen JH (1992b) Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley. Euphytica 63:141–152

    Article  Google Scholar 

  • Kalendar R, Lee D, Schulman A (2009) FastPCR software for PCR primer and probe design and repeat search. Genes Genomes Genomics 3:1–14

    Google Scholar 

  • Kato A (2011) High-density fluorescence in situ hybridization signal detection on barley (Hordeum vulgare L.) chromosomes with improved probe screening and reprobing procedures. Genome 54:151–159

    Article  PubMed  CAS  Google Scholar 

  • Kato A, Albert P, Vega J, Birchler J (2006) Sensitive fluorescence in situ hybridization signal detection in maize using directly labeled probes produced by high concentration DNA polymerase nick translation. Biotech Histochem 81:71–78

    Article  PubMed  Google Scholar 

  • Kilian A, Chen J, Han F, Steffenson B, Kleinhofs A (1997) Towards map-based cloning of the barley stem rust resistance genes Rpg1 and rpg4 using rice as an intergenomic cloning vehicle. Plant Mol Biol 35:187–195

    Article  PubMed  CAS  Google Scholar 

  • Kumar J, Hückelhoven R, Beckhove U, Nagarajan S, Kogel K-H et al (2001) A compromised Mlo pathway affects the response of barley to the necrotrophic fungus Bipolaris sorokiniana (teleomorph: Cochliobolus sativus) and its toxins. Phytopathology 91:127–133

    Article  PubMed  CAS  Google Scholar 

  • Künzel G, Korzun L, Meister A (2000) Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on translocation breakpoints. Genetics 154:397–412

    PubMed  Google Scholar 

  • Kwaaitaal M, Keinath NF, Pajonk S, Biskup C, Panstruga R et al (2010) Combined Bimolecular Fluorescence Complementation and Förster Resonance Energy Transfer reveals ternary SNARE complex formation in living plant cells. Plant Physiol 152:1135–1147

    Article  PubMed  CAS  Google Scholar 

  • Kwon C, Neu C, Pajonk S, Yun HS, Lipka U, Humphry M, Bau S, Straus M, Kwaaitaal M, Rampelt H, Kasmi FE, Jurgens G, Parker J, Panstruga R, Lipka V, Schulze-Lefert P et al (2008) Co-option of a default secretory pathway for plant immune responses. Nature 451:835–840

    Article  PubMed  CAS  Google Scholar 

  • Lahaye T, Hartmann S, Töpsch S, Freialdenhoven A, Yano M, Schulze-Lefert P et al (1998) High-resolution genetic and physical mapping of the Rar1 locus in barley. Theor Appl Genet 97:526–534

    Article  CAS  Google Scholar 

  • Langmead B, Trapnell C, Pop M, Salzberg S et al (2009) Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol 10:R25

    Article  PubMed  Google Scholar 

  • Li B, Choulet F, Heng YF, Hao WW, Paux E, Liu Z, Yue W, Jin WW, Feuillet C, Zhang XY et al (2013) Wheat centromeric retrotransposons: the new ones take a major role in centromeric structure. Plant J 73:952–965

    Article  PubMed  CAS  Google Scholar 

  • Ma L, Vu G, Schubert V, Watanabe K, Stein N, Houben A, Schubert I et al (2010) Synteny between Brachypodium distachyon and Hordeum vulgare as revealed by FISH. Chromosome Res 18:841–850

    Article  PubMed  CAS  Google Scholar 

  • Matsumoto T, Tanaka T, Sakai H, Amano N, Kanamori H, Kurita K, Kikuta A, Kamiya K, Yamamoto M, Ikawa H, Fujii N, Hori K, Itoh T, Sato K et al (2011) Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries. Plant Physiol 156:20–28

    Article  PubMed  CAS  Google Scholar 

  • Mayer KFX, Martis M, Hedley PE, Simková H, Liu H, Morris JA, Steuernagel B, Taudien S, Roessner S, Gundlach H, Kubaláková M, Suchánková P, Murat F, Felder M, Nussbaumer T, Graner A, Salse J, Endo T, Sakai H, Tanaka T, Itoh T, Sato K, Platzer M, Matsumoto T, Scholz U, Dolezel J, Waugh R, Stein N et al (2011) Unlocking the barley genome by chromosomal and comparative genomics. Plant Cell 23:1249–1263

    Article  PubMed  CAS  Google Scholar 

  • Meyer D, Pajonk S, Micali C, O’Connell R, Schulze-Lefert P et al (2009) Extracellular transport and integration of plant secretory proteins into pathogen-induced cell wall compartments. Plant J 57:986–999

    Article  PubMed  CAS  Google Scholar 

  • Ogilvie DJ, James LA (1996) End rescue from YACs using the vectorette. In: Marki D (ed) YAC Protocols, Methods in molecular biology, vol 54. Humana Press, New York, USA, pp 131–138

  • Orabi J, Backes G, Wolday A, Yahyaoui A, Jahoor A et al (2007) The horn of Africa as a centre of barley diversification and a potential domestication site. Theor Appl Genet 114:1117–1127

    Article  PubMed  CAS  Google Scholar 

  • Peterhänsel C, Freialdenhoven A, Kurth J, Kolsch R, Schulze-Lefert P et al (1997) Interaction analyses of genes required for resistance responses to powdery mildew in barley reveal distinct pathways leading to leaf cell death. Plant Cell 9:1397–1409

    PubMed  Google Scholar 

  • Piffanelli P, Zhou F, Casais C, Orme J, Jarosch B, Schaffrath U, Collins NC, Panstruga R, Schulze-Lefert P et al (2002) The barley MLO modulator of defense and cell death is responsive to biotic and abiotic stress stimuli. Plant Physiol 129:1076–1085

    Article  PubMed  CAS  Google Scholar 

  • Pourkheirandish M, Wicker T, Stein N, Fujimura T, Komatsuda T et al (2007) Analysis of the barley chromosome 2 region containing the six-rowed spike gene vrs1 reveals a breakdown of rice–barley micro collinearity by a transposition. Theor Appl Genet 114:1357–1365

    Article  PubMed  CAS  Google Scholar 

  • Robinson JT, Thorvaldsdóttir H, Winckler W, Guttman M, Lander ES, Getz G, Mesirov JP et al (2011) Integrative genomics viewer. Nat Biotechnol 29:24–26

    Article  PubMed  CAS  Google Scholar 

  • Ruiz-Lozano JM, Gianinazzi S, Gianinazzi-Pearson V (1999) Genes involved in resistance to powdery mildew in barley differentially modulate root colonization by the mycorrhizal fungus Glomus mosseae. Mycorrhiza 9:237–240

    Article  CAS  Google Scholar 

  • Schmidt D, Röder M, Dargatz H, Wolf N, Schweizer G, Tekauz A, Ganal M et al (2001) Construction of a YAC library from barley cultivar Franka and identification of YAC-derived markers linked to the Rh2 gene conferring resistance to scald (Rhynchosporium secalis). Genome 44:1031–1040

    PubMed  CAS  Google Scholar 

  • Shirasu K, Lahaye T, Tan M-W, Zhou F, Azevedo C, Schulze-Lefert P et al (1999) A novel class of eukaryotic zinc-binding proteins is required for disease resistance signaling in barley and development in C. elegans. Cell 99:355–366

    Article  PubMed  CAS  Google Scholar 

  • Shure M, Wessler S, Fedoroff N (1983) Molecular identification and isolation of the Waxy locus in maize. Cell 35:225–233

    Article  PubMed  CAS  Google Scholar 

  • Simons G, van der Lee T, Diergaarde P, van Daelen R, Groenendijk J, Frijters A, Büschges R, Hollricher K, Töpsch S, Schulze-Lefert P, Salamini F, Zabeau M, Vos P et al (1997) AFLP-based fine mapping of the Mlo gene to a 30-kb DNA segment of the barley genome. Genomics 44:61–70

    Article  PubMed  CAS  Google Scholar 

  • Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJM, Birol İ et al (2009) ABySS: a parallel assembler for short read sequence data. Genome Res 19:1117–1123

    Article  PubMed  CAS  Google Scholar 

  • Stein N, Prasad M, Scholz U, Thiel T, Zhang H, Wolf M, Kota R, Varshney R, Perovic D, Grosse I, Graner A et al (2007) A 1,000-loci transcript map of the barley genome: new anchoring points for integrative grass genomics. Theor Appl Genet 114:823–839

    Article  PubMed  CAS  Google Scholar 

  • Stoessl A, Unwin CH (1970) The antifungal factors in barley. V. Antifungal activity of the hordatines. Can J Bot 48:465–470

    Article  CAS  Google Scholar 

  • Takamatsu S (2004) Phylogeny and evolution of the powdery mildew fungi (Erysiphales, Ascomycota) inferred from nuclear ribosomal DNA sequences. Mycoscience 45:147–157

    Article  CAS  Google Scholar 

  • Taketa S, You T, Sakurai Y, Miyake S, Ichii M et al (2011) Molecular mapping of the short awn 2 (lks2) and dense spike 1 (dsp1) genes on barley chromosome 7H. Breed Sci 61:80–85

    Article  Google Scholar 

  • The International Barley Genome Consortium (2012) A physical, genetic and functional sequence assembly of the barley genome. Nature 491:711–716

    Google Scholar 

  • Trujillo M, Troeger M, Niks RE, Kogel K-H, Hückelhoven R et al (2004) Mechanistic and genetic overlap of barley host and non-host resistance to Blumeria graminis. Mol Plant Pathol 5:389–396

    Article  PubMed  CAS  Google Scholar 

  • von Röpenack E, Parr A, Schulze-Lefert P (1998) Structural analyses and dynamics of soluble and cell wall-bound phenolics in a broad spectrum resistance to the powdery mildew fungus in barley. J Biol Chem 273:9013–9022

    Article  Google Scholar 

  • Wicker T, Buchmann JP, Keller B (2010) Patching gaps in plant genomes results in gene movement and erosion of colinearity. Genome Res 20:1229–1237

    Article  PubMed  CAS  Google Scholar 

  • Wicker T, Mayer KFX, Gundlach H, Martis M, Steuernagel B, Scholz U, Šimková H, Kubaláková M, Choulet F, Taudien S, Platzer M, Feuillet C, Fahima T, Budak H, Doležel J, Keller B, Stein N et al (2011) Frequent gene movement and pseudogene evolution is common to the large and complex genomes of wheat, barley, and their relatives. Plant Cell 23:1706–1718

    Article  PubMed  CAS  Google Scholar 

  • Xu Z, Escamilla-Treviño L, Zeng L, Lalgondar M, Bevan D, Winkel B, Mohamed A, Cheng C-L, Shih M-C, Poulton J, Esen A et al (2004) Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1. Plant Mol Biol 55:343–367

    Article  PubMed  CAS  Google Scholar 

  • Zhang W-J, Pedersen C, Kwaaitaal M, Gregersen PL, Mørch SM, Hanisch S, Kristensen A, Fuglsang AT, Collinge DB, Thordal-Christensen H et al (2012) Interaction of barley powdery mildew effector candidate CSEP0055 with the defence protein PR17c. Mol Plant Pathol 13:1110–1119

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

R.P, J.A.G and M.B were funded by grants from the Max Planck Society. J.A.G was additionally supported by a research fellowship from the International Max Planck Research School (IMPRS). N.C.C gratefully acknowledges Syngenta for funding. Work in the P.B lab is supported by an EMBO Installation Grant. We are thankful to Margaret Corbitt for technical assistance, Takuji Sasaki and Robin Buell for sharing rice genomics information pre-publication, Pietro Spanu for performing pulsed field gel electrophoresis of YACs and Akio Kato for providing the probe pHv1112.

Author information

Author notes

  1. Nicholas C. Collins

    Present address: Australian Centre for Plant Functional Genomics, School of Agriculture Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia

  2. Nahal Ahmadinejad

    Present address: INRES, Crop Bioinformatics, University of Bonn, Katzenburgweg 2, 53115, Bonn, Germany

  3. Mariam Benjdia

    Present address: European Commission ERC Executive Agency, COV2, 1049, Brussels, Belgium

  4. Pawel Bednarek

    Present address: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland

Authors and Affiliations

  1. Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany

    Johanna Acevedo-Garcia, Nahal Ahmadinejad, Mariam Benjdia, Richard Reinhardt, Paul Schulze-Lefert & Ralph Panstruga

  2. Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Norwich, NR4-7UH, UK

    Nicholas C. Collins

  3. Unit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany

    Johanna Acevedo-Garcia & Ralph Panstruga

  4. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), OT Gatersleben, Corrensstrasse 3, 06466, Stadt Seeland, Germany

    Lu Ma & Andreas Houben

  5. Cologne Center for Genomics (CCG), University of Cologne, Weyertal 115b, 50931, Cologne, Germany

    Janine Altmüller & Peter Nürnberg

  6. Institute for Biology I, RWTH Aachen University, Worringer Weg 1, 52056, Aachen, Germany

    Andreas Freialdenhoven

Authors
  1. Johanna Acevedo-Garcia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nicholas C. Collins
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nahal Ahmadinejad
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lu Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Andreas Houben
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pawel Bednarek
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mariam Benjdia
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Andreas Freialdenhoven
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Janine Altmüller
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Peter Nürnberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Richard Reinhardt
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Paul Schulze-Lefert
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Ralph Panstruga
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ralph Panstruga.

Additional information

Communicated by P. M. Hayes.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 68 kb) File S1 Non-coding sequences derived from de novo assembly of YAC sequence reads

122_2013_2186_MOESM2_ESM.png

Supplementary material 2 (PNG 1536 kb) Fig. S1 Micrographs of multicolor FISH on barley metaphase chromosomes of cv. Ingrid. Shown in white (in merged) is the signal of the chromosome 1H long arm-specific probe pHv-1112 (Kato 2011); the arrows indicate the position of the signals for the genic probes in green and red; the insets show a magnification of the chromosomes with the FISH signals in black and white and after pseudo-coloring (in part rotated to suit the Figure format, arrow head indicates the pHv-1112-specific signal). Scale bar = 20 µm. a Probes for Con (red) + Pol (green) + pHv-1112. b Con (red) + Unk (green) + pHv-1112. Note that only results for combinations 1 and 2 (see main text) are shown and that the different chromosome background colors in Fig. 3 (blue) and Fig. S1 (greenish) are due to the fact that results shown in Fig. S1 are multicolor overlays of three differently labeled probes, lacking a dedicated color for the chromosomes

122_2013_2186_MOESM3_ESM.png

Supplementary material 3 (PNG 544 kb) Fig. S2 Accumulation of selected secondary metabolites in first leaves of the indicated barley genotypes at 24 and 48 hours after inoculation with Blumeria graminis f. sp. hordei spores. Compounds with retention time (RT) 10.9, 12.3, 14.1 and 16.2 minutes are shown as the ones representing the most striking induction after pathogen inoculation. Error bars indicate standard deviations of one experiment

122_2013_2186_MOESM4_ESM.png

Supplementary material 4 (PNG 148 kb) Fig. S3 UV spectra for four UPLC-PDA metabolite peaks showing induction by Blumeria graminis f. sp. hordei inoculation in primary barley leaves. Representative UV spectra of compounds eluted at retention time (RT) 14.1 and 16.2 resemble those reported for hordatines, their precursors and derivatives (Stoessl and Unwin 1970; von Röpenack et al. 1998). AU, absorbance units

Supplementary material 5 (XLSX 15 kb) Table S1 Primer pairs used to amplify the Pol and Con genes and selected YAC ends

122_2013_2186_MOESM6_ESM.xlsx

Supplementary material 6 (XLSX 17 kb) Table S2 Detail of primer pairs used to investigate the genes from the YAC clones and the genomic DNA sequence obtained for each gene fragment in the parents Ingrid and Malteria Heda

Supplementary material 7 (XLSX 11 kb) Table S3 Primer pairs used to amplify unique probes for barley FISH

122_2013_2186_MOESM8_ESM.xls

Supplementary material 8 (XLS 119 kb) Table S4 Details of DNA sequence polymorphism survey across three barley mapping parent genotypes

122_2013_2186_MOESM9_ESM.xls

Supplementary material 9 (XLS 52 kb) Table S5 Polymorphisms, haplotypes and marker details for the three barley mapping parent genotypes

122_2013_2186_MOESM10_ESM.xls

Supplementary material 10 (XLS 50 kb) Table S6 Genotypes of F2 recombinants for the Ror1 interval, used for fine mapping

122_2013_2186_MOESM11_ESM.xlsx

Supplementary material 11 (XLSX 13 kb) Table S7 Genotype in a panel of recombinants for the Ror1 interval for the genes identified in the sequenced YAC clones

122_2013_2186_MOESM12_ESM.xlsx

Supplementary material 12 (XLSX 16 kb) Table S8 Anchoring option, position coordinates and contigs in the barley genome for the genes present in the sequenced YAC clones close to the Ror1 locus. The analysis is based on the International Barley Sequencing Consortium (2012) genome draft release ftp://ftpmips.helmholtz-muenchen.de/plants/barley/public_data/ and http://webblast.ipk-gatersleben.de/barley

Rights and permissions

Reprints and permissions

About this article

Cite this article

Acevedo-Garcia, J., Collins, N.C., Ahmadinejad, N. et al. Fine mapping and chromosome walking towards the Ror1 locus in barley (Hordeum vulgare L.). Theor Appl Genet 126, 2969–2982 (2013). https://doi.org/10.1007/s00122-013-2186-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-013-2186-6

Keywords

Search

Navigation