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Molecular cloning ofTrichophyton mentagrophytes DNA sequences with promoter activity inEscherichia coli

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Abstract

A promoter probe library from the dermatophyte fungusTrichophyton mentagrophytes has been constructed in the pVB32 plasmid vector. Using this library, a set ofT. mentagrophytes DNA sequences with promoter activity inEscherichia coli has been cloned. The size and the resistance phenotype conferred by these DNA fragments varied. Southern blot analysis confirmed that they were derived fromT. mentagrophytes genomic DNA.

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Authors and Affiliations

  1. Departament de Microbiologia, Facultat de Ciències Biològiques, Universitat de València, Calle Dr. Moliner 50, 46100, Valencia, Spain

    C. Tortajada & S. Ferrer

  2. Unidad de Bioingenieria, Instituto de Agroquimica y Tecnologia de los Alimentos (C.S.I.C.), Calle Jaime Roig 11, 46010, Valencia, Spain

    D. Ramon

Authors
  1. C. Tortajada
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  2. S. Ferrer
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  3. D. Ramon
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Tortajada, C., Ferrer, S. & Ramon, D. Molecular cloning ofTrichophyton mentagrophytes DNA sequences with promoter activity inEscherichia coli . World J Microbiol Biotechnol 8, 196–198 (1992). https://doi.org/10.1007/BF01195847

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  • DOI: https://doi.org/10.1007/BF01195847

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