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Toward an Alternative Therapeutic Approach for Skin Infections: Antagonistic Activity of Lactobacilli Against Antibiotic-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

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Abstract

The wide spread of antimicrobial resistance has urged the need of alternative therapeutic approach. In this context, probiotic lactobacilli have been reported for the prevention and treatment of many gastrointestinal and urogenital infections. However, very little is known about their antagonistic activity against skin pathogens. Accordingly, the present study aimed to investigate the potential of lactobacilli to interfere with pathogenesis features of two antibiotic-resistant skin pathogens, namely methicillin-resistant Staphylococcus aureus and multiple-resistant Pseudomonas aeruginosa. A total of 49 lactobacilli were recovered, identified and tested for their antagonistic activities against the aforementioned pathogens. Of these, eight isolates were capable of blocking the adherence of pathogens to mammalian cells independent of the skin pathogen tested or model adopted. Moreover, three Lactobacillus isolates (LRA4, LC2 and LR5) effectively prevented the pathogen internalization into epithelial cells in addition to potentiating phagocyte-mediated pathogen killing. Interestingly, the lactobacilli LC2, LF9 and LRA4 markedly inhibited the growth of P. aeruginosa and S. aureus isolates in coculture experiments. Besides, the lactobacilli LRA4, LC2, LR5 and LF9 have counteracted pathogen cytotoxicity. Taken together, the present study revealed some inhibitory activities of lactobacilli against two antibiotic-resistant skin pathogens. Moreover, it revealed two lactobacilli, namely LC2 and LRA4, with antagonistic capacity against different virulence determinants of skin pathogens. These lactobacilli are considered promising probiotic candidates that may represent an alternative therapeutic approach for skin infections.

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Acknowledgments

The authors are grateful to Taif university, KSA, for funding this work under university project No. 1257.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Pharmaceutical Microbiology, College of Pharmacy, Taif University, Taif, Western Region, KSA

    Mohamed M. Hafez

  2. Department of Clinical Pharmacy, College of Pharmacy, Taif University, Taif, KSA

    Ibrahim A. Maghrabi

  3. Department of Pharmaceutics, College of Pharmacy, Taif University, Taif, KSA

    Noha M. Zaki

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  1. Mohamed M. Hafez
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  2. Ibrahim A. Maghrabi
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  3. Noha M. Zaki
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Correspondence to Mohamed M. Hafez.

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Hafez, M.M., Maghrabi, I.A. & Zaki, N.M. Toward an Alternative Therapeutic Approach for Skin Infections: Antagonistic Activity of Lactobacilli Against Antibiotic-Resistant Staphylococcus aureus and Pseudomonas aeruginosa . Probiotics & Antimicro. Prot. 5, 216–226 (2013). https://doi.org/10.1007/s12602-013-9137-z

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