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Competence and sporulation factor derived from Bacillus subtilis improves epithelial cell injury in intestinal inflammation via immunomodulation and cytoprotection

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Abstract

Introduction

Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. These probiotic effects are considered to be displayed through the mediation of effective molecules derived from these bacteria because live bacteria as well as their conditioned media exhibit beneficial effects in many cases. However, many of the probiotic-derived molecules which mediate such benefits have so far been poorly characterized. We previously found that competence and sporulation factor (CSF) activates the Akt and p38 MAPK pathways and protects epithelial cells from oxidant stress in the mammalian intestine. The purpose of this study is to determine the CSF effect on reducing intestinal inflammation.

Methods and results

A protein array demonstrated that CSF induced the anti-inflammatory cytokine, IL-10, and decreased the release of pro-inflammatory mediators, IL-4, IL-6 and CXCL-1, induced by TNF-α in Caco2/bbe cells. CSF also induced the cytoprotective protein Hsp 27 in Caco2/bbe cells. The histological score of intestinal inflammation in 2% dextran sodium sulfate (DSS)-treated mice with the administration of 10 nM CSF was significantly lower than that of control mice. CSF also improved the survival rate of mice treated with a lethal concentration of DSS.

Conclusion

Therefore, CSF is a potentially effective treatment for intestinal inflammation.

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Acknowledgments

The study was supported by a Grant-in-Aid for Scientific Research no. 20590734 (M.F.) and the Intractable Disease, the Health and Labour Sciences Research Grants from the Ministry of Health, Labor and Welfare.

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Authors and Affiliations

  1. Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1, Midorigaoka-higashi, Asahikawa, Hokkaido, 078-8510, Japan

    Kotaro Okamoto, Mikihiro Fujiya, Toshie Nata, Nobuhiro Ueno, Yuhei Inaba, Chisato Ishikawa, Takahiro Ito, Kentaro Moriichi, Hiroki Tanabe, Yusuke Mizukami & Yutaka Kohgo

  2. Martin Boyer Laboratories, University of Chicago IBD Research Center, 5841 S. Maryland Ave. AMB G705 (MC 6084), Chicago, IL, 60637, USA

    Eugene B. Chang

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  1. Kotaro Okamoto
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  2. Mikihiro Fujiya
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Correspondence to Mikihiro Fujiya.

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Okamoto, K., Fujiya, M., Nata, T. et al. Competence and sporulation factor derived from Bacillus subtilis improves epithelial cell injury in intestinal inflammation via immunomodulation and cytoprotection. Int J Colorectal Dis 27, 1039–1046 (2012). https://doi.org/10.1007/s00384-012-1416-8

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