Abstract
Cell cultures established from plants represent an attractive alternative to whole plants for effective production of bioactive secondary metabolites. Cell culture from Vitis vinifera L. cv. Gamay Fréaux accumulated high amounts of hydroxycinnamic acid derivatives and anthocyanins. Two new compounds were identified: 3-O-glucosylresveratrol, a stilbene derivative, abundant in cell suspension culture, and a hydroxyphenol, 4-(3,5-dihydroxyphenyl)-phenol, abundant in callus culture. The major anthocyanin monoglucosides present in cell suspension culture were cyanidin 3-O-glucoside and peonidin 3-O-glucoside, and the major cinnamoyl derivatives were cyanidin 3-O-p-coumaryl glucoside and peonidin 3-O-p-coumaryl glucoside. Three minor anthocyanin compounds were found in V. vinifera cell culture: delphinidin 3-O-glucoside, petunidin 3-O-glucoside, and delphinidin 3-O-p-coumaryl glucoside. Anthocyanin levels of cell suspension cultures increased significantly—about eight fold—after 4-day cultivation in new medium. Salicylic acid at a concentration of 50 μM did not enhance anthocyanin accumulation in cell suspension culture, and similar levels of jasmonic acid significantly reduced the anthocyanin content.
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Acknowledgments
We would like to thank Heidi Riedel and Onur Kütük from the Berlin University of Technology for providing phenolic acid and anthocyanin extracts for the fractioning procedure. We also acknowledge the technical assistance of Dr. Michael Reichelt from Max-Planck-Institute for Chemical Ecology in Jena. We would also like to express our gratitude to Prof. Dietrich Knorr and Irene Hemmerich from the Berlin University of Technology for providing V. vinifera cell culture and their continuous cultivation.
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Mewis, I., Smetanska, I.M., Müller, C.T. et al. Specific Poly-phenolic Compounds in Cell Culture of Vitis vinifera L. cv. Gamay Fréaux. Appl Biochem Biotechnol 164, 148–161 (2011). https://doi.org/10.1007/s12010-010-9122-x
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DOI: https://doi.org/10.1007/s12010-010-9122-x