Summary
Anthocyanin production of two lines ofVitis vinifera cell cultures, i.e., 5.4 and 13.1, which were obtained from the same starting material after 20 and 37 mo. of clonal selection, respectively, was investigated. Cell suspension cultures of lines 5.4 and 13.1 maintained an anthocyanin content of 0.44 ± 0.15 and 1.02 ± 0.31 mg·g−1 fresh weight during 50 and 32 weekly maintenance subcultures, respectively. Under anthocyanin-promoting culture conditions, both lines showed an enhancement of their anthocyanin level by approximately fourfold. While line 5.4 accumulated peonidin 3-glucoside and cyanidin 3-glucoside in decreasing order, line 13.1 accumulated primarily peonidin 3-p-coumaroylglucoside with lesser amounts of malvidin monoglucoside. Results show that while the anthocyanin content was improved during the course of repeated selections, the anthocyanin composition was modified markedly favoring the accumulation of more metabolically-advanced anthocyanins.
Similar content being viewed by others
References
Cormier, F.; Crevier, H. A.; Do, C. B. Effects of sucrose on the accumulation of anthocyanins in grape (Vitis vinifera) cell suspensions. Can. J. Bot. 68:1822–1826; 1990.
Cormier, F.; Do, C. B. XXVIIVitis vinifera L. (Grapevine): in vitro production of anthocyanins. In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry, vol. 24. Medicinal and aromatic plants V. Berlin: Springer-Verlag; 1993:373–386.
Do, C. B.; Cormier, F. Accumulation of peonidin 3-glucoside enhanced by osmotic stress in grape (Vitis vinifera) cell suspension. Plant Cell Tissue Organ Cult. 24:49–54; 1991a.
Do, C. B.; Cormier, F. Effects of low nitrate and high sugar concentrations on anthocyanin content and composition of grape (Vitis vinifera L.) cell suspension. Plant Cell Rep. 9:500–504; 1991b.
Dougall, D. K. Cell cloning and the selection of high yielding strains. In: Constabel, F.; Vasil, I. K., eds. Cell culture and somatic cell genetics of plants, vol. 4. Cell culture in phytochemistry. New York: Academic Press; 1987:117–124.
Francis, J. F. Food colorants: anthocyanins. In: Clydesdale, F. M., ed. CRC Critical Reviews in Food Science and Nutrition, vol. 28, issue 4. Boca Raton, FL: CRC Press; 1989:273–314.
Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158; 1968.
Harborne, J. B. Phytochemical methods—a guide to modern techniques of plant analysis. London: Chapman and Hall; 1984.
Heller, W.; Forkmann, G. Biosynthesis. In: Harborne, J. B., ed. The flavonoids: advances in research since 1980. London: Chapman and Hall, 1988:399–425.
Koes, R. E.; van Blokland, R.; Quattrocchio, F., et al. Chalcone synthase promoters in Petunia are active in pigmented and unpigmented cell types. Plant Cell 2:379–392; 1990.
Latché, A.; Fallot, J. Obtention d’anthocyanes marquées au carbone 14 par voie biologique à l’aide de cellules de raisin cultivées in vitro. Compte-rendu contrat DGRST no 81C1376. Toulouse, France: 1984.
Lofty, S.; Fleuriet, A.; Ramos, T., et al. Biosynthesis of phenolic compounds inVitis vinifera cell suspension cultures: study on hydroxycinnamoyl CoA ligase. Plant Cell Rep. 8:93–96; 1989.
Nozue, M.; Kawai, J.; Yoshitama, K. Selection of a high anthocyanin-producing cell line of sweet potato cell cultures and identification of pigments. J. Plant Physiol. 129:81–88; 1987.
SAS Institute Inc. SAS/STAT guide for personal computers, version 6. Cary, NC: SAS Institute Inc.; 1987.
Teusch, M.; Forkmann, G.; Seyffert, W. Genetic control of hydroxycinnamoyl CoA: anthocyanidin 3-glucoside hydroxycinnamoyltransferase from petals ofMatthiola incana. Phytochemistry 26:991–994; 1987.
Van Calsteren, M.-R.; Cormier, F.; Do, C. B., et al.1H and13C NMR assignments of the major anthocyanins fromVitis vinifera cell suspension cultures. Spectroscopy 9:1–15; 1991.
Vogelien, D. L.; Hrazdina, G.; Reeves, S., et al. Phenotypic differences in anthocyanin accumulation among clonally related cultured cells of carrot. Plant Cell Tissue Organ Cult. 22:213–222; 1990.
Yamamoto, Y.; Mizuguchi, R. Selection of a high and stable pigment-producing strain in culturedEuphorbia millii cells. Theor. Appl. Genet. 61:113–116; 1982.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cormier, F., Do, C.B. & Nicolas, Y. Anthocyanin production in selected cell lines of grape (Vitis vinifera L.). In Vitro Cell Dev Biol - Plant 30, 171–173 (1994). https://doi.org/10.1007/BF02632209
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02632209