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Selection of a high and stable pigment-producing strain in cultured Euphorbia millii cells

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Summary

A high pigment-producing strain of cultured Euphorbia millii cells was isolated by clonal selection. The pigment obtained was red and consisted mainly of anthocyanin. The amount of this pigment obtained after 24 selections was seven times that found in the original cells. Statistical and cell-pedigree analyses proved that this cell strain has stable productivity for this red pigment.

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Literature

  • Matsumoto, T.; Ikeda, T.; Kanno, N.; Kisaki, T.; Noguchi, M. (1980): Selection of high ubiquinone 10-producing strains of tobacco cultured cells by cell cloning technique. Agric. Biol. Chem. 44, 967–969

    Google Scholar 

  • Murashige, T.; Skoog, F. (1962): A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol. Plant. 15, 473–497

    Google Scholar 

  • Ogino, T.; Hiraoka, N.; Tabata, M. (1978): Selection of high nicotine-producing cell lines of tobacco callus by single-cell cloning. Phytochemistry 17, 1907–1910

    Google Scholar 

  • Yamada, Y.; Watanabe, K. (1980): Selection of high vitamin B6 producing strains in cultured green cells. Agric. Biol. Chem. 44, 2683–2687

    Google Scholar 

  • Yamamoto, Y.; Mizuguchi, R.; Yamada, Y. (1981): Chemical constituents of cultured cells of Euphorbia tirucalli and E. millii. Plant Cell Rep. (in press)

  • Zenk, M.H.; El-shagi, H.; Arens, H.; Stöckgt, J.; Weiber, E.W.; Bues, B. (1977): Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Plant Tissue Culture and its Biotechnological Association (eds. Barz, W.; Reinhard, E.; Zenk, M.H.), pp. 27–43. Berlin, Heidelberg, New York: Springer

    Google Scholar 

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Communicated by K. Tsunewaki

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Yamamoto, Y., Mizuguchi, R. & Yamada, Y. Selection of a high and stable pigment-producing strain in cultured Euphorbia millii cells. Theoret. Appl. Genetics 61, 113–116 (1982). https://doi.org/10.1007/BF00273877

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  • DOI: https://doi.org/10.1007/BF00273877

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