Abstract
Humans, in contrast to other mammals, do not synthesize N-glycolyl-neuraminic acid (Neu5Gc) due to a deletion in the gene (cmah) encoding the enzyme responsible for this conversion, the cytidine monophospho-N-acetyl-neuraminic acid hydroxylase (CMP-Neu5Ac hydroxylase). The detection of considerable amounts of Neu5Gc-sialoconjugates, in particular gangliosides, in human malignancies makes these antigens attractive targets for immunotherapy, in particular with monoclonal antibodies (mAbs). We have previously described a GM3(Neu5Gc) ganglioside-specific mAb, named 14F7, with the ability to kill tumor cells in a complement-independent manner. Silencing the cmah gene in GM3(Neu5Gc)-expressing L1210 mouse lymphocytic leukemia B cells caused the abrogation of this cytotoxic effect. We now show that cmah-silenced L1210 cells (cmah-kd) express a high level of GM3(Neu5Ac) and have an impaired ability for anchorage-independent cell growth and tumor development in vivo. No evidences of increased immunogenicity of the cmah-kd cell line were found. These results provide new evidences on the role of GM3(Neu5Gc), or Neu5Gc-sialoconjugates in general, in tumor biology. As an important tool in this study, we used the humanized version (here referred to as 7C1 mAb) of a recently described, rationally-designed mutant of 14F7 mAb that is able to bind to both GM3(Neu5Gc) and GM3(Neu5Ac). In contrast to its parental antibody, the humanized 14F7 (14F7hT) mAb, 7C1 mAb was able to kill not only GM3(Neu5Gc)-expressing L1210 wild type cells, but also GM3(Neu5Ac)-expressing cmah-kd cells, which endorses this antibody as a potential agent for cancer immunotherapy.
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References
Hakomori, S.: Structure, organization, and function of glycosphingolipids in membrane. Curr. Opin. Hematol. 10(1), 16–24 (2003)
Lopez, P.H., Schnaar, R.L.: Gangliosides in cell recognition and membrane protein regulation. Curr. Opin. Struct. Biol. 19(5), 549–557 (2009). doi:10.1016/j.sbi.2009.06.001
Sonnino, S., Mauri, L., Chigorno, V., Prinetti, A.: Gangliosides as components of lipid membrane domains. Glycobiology 17(1), 1R–13R (2007). doi:10.1093/glycob/cwl052
Sonnino, S., Prinetti, A.: Lipids and membrane lateral organization. Front. Physiol. 1, 153 (2010). doi:10.3389/fphys.2010.00153
Cantu, L., Del Favero, E., Sonnino, S., Prinetti, A.: Gangliosides and the multiscale modulation of membrane structure. Chem. Phys. Lipids 164(8), 796–810 (2011). doi:10.1016/j.chemphyslip.2011.09.005
Hakomori, S.: Tumor malignancy defined by aberrant glycosylation and sphingo(glyco)lipid metabolism. Cancer Res. 56(23), 5309–5318 (1996)
Prinetti, A., Prioni, S., Loberto, N., Aureli, M., Nocco, V., Illuzzi, G., Mauri, L., Valsecchi, M., Chigorno, V., Sonnino, S.: Aberrant glycosphingolipid expression and membrane organization in tumor cells: consequences on tumor-host interactions. Adv. Exp. Med. Biol. 705, 643–667 (2011). doi:10.1007/978-1-4419-7877-6_34
Durrant, L.G., Noble, P., Spendlove, I.: Immunology in the clinic review series; focus on cancer: glycolipids as targets for tumour immunotherapy. Clin. Exp. Immunol. 167(2), 206–215 (2012). doi:10.1111/j.1365-2249.2011.04516.x
Rabu, C., McIntosh, R., Jurasova, Z., Durrant, L.: Glycans as targets for therapeutic antitumor antibodies. Future Oncol. 8(8), 943–960 (2012). doi:10.2217/fon.12.88
Higashi, H., Hirabayashi, Y., Fukui, Y., Naiki, M., Matsumoto, M., Ueda, S., Kato, S.: Characterization of N-glycolylneuraminic acid-containing gangliosides as tumor-associated Hanganutziu-Deicher antigen in human colon cancer. Cancer Res. 45(8), 3796–3802 (1985)
Marquina, G., Waki, H., Fernandez, L.E., Kon, K., Carr, A., Valiente, O., Perez, R., Ando, S.: Gangliosides expressed in human breast cancer. Cancer Res. 56(22), 5165–5171 (1996)
Varki, A.: Multiple changes in sialic acid biology during human evolution. Glycoconj. J. 26(3), 231–245 (2009). doi:10.1007/s10719-008-9183-z
Irie, A., Koyama, S., Kozutsumi, Y., Kawasaki, T., Suzuki, A.: The molecular basis for the absence of N-glycolylneuraminic acid in humans. J. Biol. Chem. 273(25), 15866–15871 (1998)
Tangvoranuntakul, P., Gagneux, P., Diaz, S., Bardor, M., Varki, N., Varki, A., Muchmore, E.: Human uptake and incorporation of an immunogenic nonhuman dietary sialic acid. Proc. Natl. Acad. Sci. U. S. A. 100(21), 12045–12050 (2003). doi:10.1073/pnas.2131556100
Bardor, M., Nguyen, D.H., Diaz, S., Varki, A.: Mechanism of uptake and incorporation of the non-human sialic acid N-glycolylneuraminic acid into human cells. J. Biol. Chem. 280(6), 4228–4237 (2005). doi:10.1074/jbc.M412040200
Banda, K., Gregg, C.J., Chow, R., Varki, N.M., Varki, A.: Metabolism of vertebrate amino sugars with N-glycolyl groups: mechanisms underlying gastrointestinal incorporation of the non-human sialic acid xeno-autoantigen N-glycolylneuraminic acid. J. Biol. Chem. 287(34), 28852–28864 (2012). doi:10.1074/jbc.M112.364182
Hedlund, M., Tangvoranuntakul, P., Takematsu, H., Long, J.M., Housley, G.D., Kozutsumi, Y., Suzuki, A., Wynshaw-Boris, A., Ryan, A.F., Gallo, R.L., Varki, N., Varki, A.: N-glycolylneuraminic acid deficiency in mice: implications for human biology and evolution. Mol. Cell. Biol. 27(12), 4340–4346 (2007). doi:10.1128/MCB.00379-07
Yin, J., Hashimoto, A., Izawa, M., Miyazaki, K., Chen, G.Y., Takematsu, H., Kozutsumi, Y., Suzuki, A., Furuhata, K., Cheng, F.L., Lin, C.H., Sato, C., Kitajima, K., Kannagi, R.: Hypoxic culture induces expression of sialin, a sialic acid transporter, and cancer-associated gangliosides containing non-human sialic acid on human cancer cells. Cancer Res. 66(6), 2937–2945 (2006). doi:10.1158/0008-5472.CAN-05-2615
Yin, J., Miyazaki, K., Shaner, R.L., Merrill Jr., A.H., Kannagi, R.: Altered sphingolipid metabolism induced by tumor hypoxia—new vistas in glycolipid tumor markers. FEBS Lett. 584(9), 1872–1878 (2010). doi:10.1016/j.febslet.2009.11.019
Carr, A., Mullet, A., Mazorra, Z., Vazquez, A.M., Alfonso, M., Mesa, C., Rengifo, E., Perez, R., Fernandez, L.E.: A mouse IgG1 monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors. Hybridoma 19(3), 241–247 (2000). doi:10.1089/02724570050109639
Oliva, J.P., Valdes, Z., Casaco, A., Pimentel, G., Gonzalez, J., Alvarez, I., Osorio, M., Velazco, M., Figueroa, M., Ortiz, R., Escobar, X., Orozco, M., Cruz, J., Franco, S., Diaz, M., Roque, L., Carr, A., Vazquez, A.M., Mateos, C., Rubio, M.C., Perez, R., Fernandez, L.E.: Clinical evidences of GM3 (NeuGc) ganglioside expression in human breast cancer using the 14F7 monoclonal antibody labelled with (99 m)Tc. Breast Cancer Res. Treat. 96(2), 115–121 (2006). doi:10.1007/s10549-005-9064-0
Fernandez-Marrero, Y., Roque-Navarro, L., Hernandez, T., Dorvignit, D., Molina-Perez, M., Gonzalez, A., Sosa, K., Lopez-Requena, A., Perez, R., Mateo de Acosta, C.: A cytotoxic humanized anti-ganglioside antibody produced in a murine cell line defective of N-glycolylated-glycoconjugates. Immunobiology (2011). doi: 10.1016/j.imbio.2011.07.004
Osorio, M., Gracia, E., Rodriguez, E., Saurez, G., Arango Mdel, C., Noris, E., Torriella, A., Joan, A., Gomez, E., Anasagasti, L., Gonzalez, J.L., Melgares Mde, L., Torres, I., Gonzalez, J., Alonso, D., Rengifo, E., Carr, A., Perez, R., Fernandez, L.E.: Heterophilic NeuGcGM3 ganglioside cancer vaccine in advanced melanoma patients: results of a Phase Ib/IIa study. Cancer Biol. Ther. 7(4), 488–495 (2008)
Blanco, R., Rengifo, E., Rengifo, C.E., Cedeno, M., Frometa, M., Carr, A.: Immunohistochemical reactivity of the 14F7 monoclonal antibody raised against N-glycolyl GM3 ganglioside in some benign and malignant skin neoplasms. ISRN Dermatol. 2011, 848909 (2011). doi:10.5402/2011/848909
Zhong, Y., Wu, Y., Li, C., Tang, J., Wang, X., Ren, G., Carr, A., Perez, R., Guo, W.: N-Glycolyl GM3 ganglioside immunoexpression in oral mucosal melanomas of Chinese. Oral Dis. 18(8), 741–747 (2012). doi:10.1111/j.1601-0825.2012.01939.x
van Cruijsen, H., Ruiz, M.G., van der Valk, P., de Gruijl, T.D., Giaccone, G.: Tissue micro array analysis of ganglioside N-glycolyl GM3 expression and signal transducer and activator of transcription (STAT)-3 activation in relation to dendritic cell infiltration and microvessel density in non-small cell lung cancer. BMC Cancer 9, 180 (2009). doi:10.1186/1471-2407-9-180
Blanco, R., Rengifo, C.E., Cedeno, M., Frometa, M., Rengifo, E., Carr, A.: Immunoreactivity of the 14F7 mab (raised against N-glycolyl GM3 ganglioside) as a positive prognostic factor in non-small-cell lung cancer. Patholog. Res. Int. 2012, 235418 (2012). doi:10.1155/2012/235418
Hayashi, N., Chiba, H., Kuronuma, K., Go, S., Hasegawa, Y., Takahashi, M., Gasa, S., Watanabe, A., Hasegawa, T., Kuroki, Y., Inokuchi, J., Takahashi, H.: Detection of N-glycolyated gangliosides in non-small-cell lung cancer using GMR8 monoclonal antibody. Cancer Sci. 104(1), 43–47 (2013). doi:10.1111/cas.12027
Scursoni, A.M., Galluzzo, L., Camarero, S., Pozzo, N., Gabri, M.R., de Acosta, C.M., Vazquez, A.M., Alonso, D.F., de Davila, M.T.: Detection and characterization of N-glycolyated gangliosides in Wilms tumor by immunohistochemistry. Pediatr. Dev. Pathol. 13(1), 18–23 (2010). doi:10.2350/08-10-0544.1
Scursoni, A.M., Galluzzo, L., Camarero, S., Lopez, J., Lubieniecki, F., Sampor, C., Segatori, V.I., Gabri, M.R., Alonso, D.F., Chantada, G., de Davila, M.T.: Detection of N-glycolyl GM3 ganglioside in neuroectodermal tumors by immunohistochemistry: an attractive vaccine target for aggressive pediatric cancer. Clin. Dev. Immunol. 2011, 245181 (2011). doi:10.1155/2011/245181
Sampor, C., Guthmann, M.D., Scursoni, A., Cacciavillano, W., Torbidoni, A., Galluzzo, L., Camarero, S., Lopez, J., de Davila, M.T., Fainboim, L., Chantada, G.L.: Immune response to racotumomab in a child with relapsed neuroblastoma. Front. Oncol. 2, 195 (2012). doi:10.3389/fonc.2012.00195
Blanco, R., Quintana, Y., Blanco, D., Cedeño, M., Rengifo, C.E., Frómeta, M., Ríos, M., Rengifo, E., Carr, A.: Tissue reactivity of the 14F7 mab raised against N-Glycolyl GM3 ganglioside in tumors of neuroectodermal, mesodermal, and epithelial origin. J. Biomarkers 2013, 602417 (2013). doi:10.1155/2013/602417
Blanco, R., Rengifo, E., Cedeno, M., Rengifo, C.E., Alonso, D.F., Carr, A.: Immunoreactivity of the 14F7 mab raised against N-glycolyl GM3 ganglioside in epithelial malignant tumors from digestive system. ISRN Gastroenterol. 2011, 645641 (2011). doi:10.5402/2011/645641
Blanco, R., Cedeño, M., Escobar, X., Blanco, D., Rengifo, C.E., Frómeta, M., Alvarez, R.I., Rengifo, E., Carr, A.: Immunorecognition of the 14F7 mab raised against N-glycolyl GM3 ganglioside in some normal and malignant tissues from genitourinary system. ISRN Pathology 2011, 953803 (2011). doi:10.5402/2011/953803
Fernandez, L.E., Gabri, M.R., Guthmann, M.D., Gomez, R.E., Gold, S., Fainboim, L., Gomez, D.E., Alonso, D.F.: NGcGM3 ganglioside: a privileged target for cancer vaccines. Clin. Dev. Immunol. 2010, 814397 (2010). doi:10.1155/2010/814397
Carr, A., Rodriguez, E., Arango Mdel, C., Camacho, R., Osorio, M., Gabri, M., Carrillo, G., Valdes, Z., Bebelagua, Y., Perez, R., Fernandez, L.E.: Immunotherapy of advanced breast cancer with a heterophilic ganglioside (NeuGcGM3) cancer vaccine. J. Clin. Oncol. 21(6), 1015–1021 (2003)
Mulens, V., de la Torre, A., Marinello, P., Rodriguez, R., Cardoso, J., Diaz, R., O’Farrill, M., Macias, A., Viada, C., Saurez, G., Carr, A., Crombet, T., Mazorra, Z., Perez, R., Fernandez, L.E.: Immunogenicity and safety of a NeuGcGM3 based cancer vaccine: results from a controlled study in metastatic breast cancer patients. Hum. Vaccin. 6(9) (2010). doi:10.4161.hv.6.9.12571
Perez, K., Osorio, M., Hernandez, J., Carr, A., Fernandez, L.E.: NGcGM3/VSSP vaccine as treatment for melanoma patients. Hum. Vaccin. Immunother. 9(6) (2013)
Vazquez, A.M., Hernandez, A.M., Macias, A., Montero, E., Gomez, D.E., Alonso, D.F., Gabri, M.R., Gomez, R.E.: Racotumomab: an anti-idiotype vaccine related to N-glycolyl-containing gangliosides—preclinical and clinical data. Front. Oncol. 2, 150 (2012). doi:10.3389/fonc.2012.00150
Vazquez, A.M., Alfonso, M., Lanne, B., Karlsson, K.A., Carr, A., Barroso, O., Fernandez, L.E., Rengifo, E., Lanio, M.E., Alvarez, C., et al.: Generation of a murine monoclonal antibody specific for N-glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids. Hybridoma 14(6), 551–556 (1995)
Carr, A., Mesa, C., del Carmen Arango, M., Vazquez, A.M., Fernandez, L.E.: In vivo and in vitro anti-tumor effect of 14F7 monoclonal antibody. Hybrid Hybridomics 21(6), 463–468 (2002). doi:10.1089/153685902321043990
Roque-Navarro, L., Chakrabandhu, K., de Leon, J., Rodriguez, S., Toledo, C., Carr, A., de Acosta, C.M., Hueber, A.O., Perez, R.: Anti-ganglioside antibody-induced tumor cell death by loss of membrane integrity. Mol. Cancer Ther. 7(7), 2033–2041 (2008). doi:10.1158/1535-7163.MCT-08-0222
Mateo, C., Lombardero, J., Moreno, E., Morales, A., Bombino, G., Coloma, J., Wims, L., Morrison, S.L., Perez, R.: Removal of amphipathic epitopes from genetically engineered antibodies: production of modified immunoglobulins with reduced immunogenicity. Hybridoma 19(6), 463–471 (2000). doi:10.1089/027245700750053959
Fernandez-Marrero, Y., Lopez-Requena, A.: Lonely killers: Effector cell- and complement-independent non-proapoptotic cytotoxic antibodies inducing membrane lesions. MAbs 3(6), 528–534 (2011). doi:10.4161/mabs.3.6.17770
Fernandez-Marrero, Y., Hernandez, T., Roque-Navarro, L., Talavera, A., Moreno, E., Grinan, T., Vazquez, A.M., de Acosta, C.M., Perez, R., Lopez-Requena, A.: Switching on cytotoxicity by a single mutation at the heavy chain variable region of an anti-ganglioside antibody. Mol. Immunol. 48(8), 1059–1067 (2011). doi:10.1016/j.molimm.2011.01.008
Rojas, G., Pupo, A., Gomez, S., Krengel, U., Moreno, E.: Engineering the binding site of an antibody against N-glycolyl GM3: from functional mapping to novel anti-ganglioside specificities. ACS Chem. Biol. 8(2), 376–386 (2013). doi:10.1021/cb3003754
Vazquez, A.M., Tormo, B., Velandia, A., Giscombe, R., Ansotegui, I., Jeddi-Tehrani, M., Perez, R., Mellstedt, H., Biberfeld, P.: Characterization of the colorectal antigen IOR-C2. Hybridoma 11(2), 245–256 (1992)
Roque-Navarro, L., Mateo, C., Lombardero, J., Mustelier, G., Fernandez, A., Sosa, K., Morrison, S.L., Perez, R.: Humanization of predicted T-cell epitopes reduces the immunogenicity of chimeric antibodies: new evidence supporting a simple method. Hybrid Hybridomics 22(4), 245–257 (2003). doi:10.1089/153685903322328974
de Leon, J., Fernandez, A., Mesa, C., Clavel, M., Fernandez, L.E.: Role of tumour-associated N-glycolylated variant of GM3 ganglioside in cancer progression: effect over CD4 expression on T cells. Cancer Immunol. Immunother. 55(4), 443–450 (2006). doi:10.1007/s00262-005-0041-6
Svennerholm, L.: Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method. Biochim. Biophys. Acta 24(3), 604–611 (1957)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1), 265–275 (1951)
Lopez-Requena, A., Mateo de Acosta, C., Perez, A., Valle, A., Lombardero, J., Sosa, K., Perez, R., Vazquez, A.M.: Chimeric anti-N-glycolyl-ganglioside and its anti-idiotypic MAbs: immunodominance of their variable regions. Hybrid Hybridomics 22(4), 235–243 (2003)
Meisen, I., Peter-Katalinic, J., Muthing, J.: Direct analysis of silica gel extracts from immunostained glycosphingolipids by nanoelectrospray ionization quadrupole time-of-flight mass spectrometry. Anal. Chem. 76(8), 2248–2255 (2004). doi:10.1021/ac035511t
Lemaire, R., Tabet, J.C., Ducoroy, P., Hendra, J.B., Salzet, M., Fournier, I.: Solid ionic matrixes for direct tissue analysis and MALDI imaging. Anal. Chem. 78(3), 809–819 (2006). doi:10.1021/ac0514669
Strohalm, M., Kavan, D., Novak, P., Volny, M., Havlicek, V.: mMass 3: a cross-platform software environment for precise analysis of mass spectrometric data. Anal. Chem. 82(11), 4648–4651 (2010). doi:10.1021/ac100818g
Cycon, K.A., Clements, J.L., Holtz, R., Fuji, H., Murphy, S.P.: The immunogenicity of L1210 lymphoma clones correlates with their ability to function as antigen-presenting cells. Immunology 128(1 Suppl), e641–e651 (2009). doi:10.1111/j.1365-2567.2009.03052.x
Portoukalian, J., Zwingelstein, G., Dore, J.F.: Lipid composition of human malignant melanoma tumors at various levels of malignant growth. Eur. J. Biochem. 94(1), 19–23 (1979)
Tsuchida, T., Saxton, R.E., Morton, D.L., Irie, R.F.: Gangliosides of human melanoma. J. Natl. Cancer. Inst. 78(1), 45–54 (1987)
Ravindranath, M.H., Muthugounder, S., Presser, N., Selvan, S.R., Santin, A.D., Bellone, S., Saravanan, T.S., Morton, D.L.: Immunogenic gangliosides in human ovarian carcinoma. Biochem. Biophys. Res. Commun. 353(2), 251–258 (2007). doi:10.1016/j.bbrc.2006.12.001
Dohi, T., Nores, G., Hakomori, S.: An IgG3 monoclonal antibody established after immunization with GM3 lactone: immunochemical specificity and inhibition of melanoma cell growth in vitro and in vivo. Cancer Res. 48(20), 5680–5685 (1988)
Hoon, D.S., Wang, Y., Sze, L., Kanda, H., Watanabe, T., Morrison, S.L., Morton, D.L., Irie, R.F.: Molecular cloning of a human monoclonal antibody reactive to ganglioside GM3 antigen on human cancers. Cancer Res. 53(21), 5244–5250 (1993)
Takahashi, T., Johnson, T.D., Nishinaka, Y., Morton, D.L., Irie, R.F.: IgM anti-ganglioside antibodies induced by melanoma cell vaccine correlate with survival of melanoma patients. J. Invest. Dermatol. 112(2), 205–209 (1999). doi:10.1046/j.1523-1747.1999.00493.x
Irie, R.F., Ollila, D.W., O’Day, S., Morton, D.L.: Phase I pilot clinical trial of human IgM monoclonal antibody to ganglioside GM3 in patients with metastatic melanoma. Cancer Immunol. Immunother. 53(2), 110–117 (2004). doi:10.1007/s00262-003-0436-1
Hakomori, S.I.: Glycosynaptic microdomains controlling tumor cell phenotype through alteration of cell growth, adhesion, and motility. FEBS Lett. 584(9), 1901–1906 (2010). doi:10.1016/j.febslet.2009.10.065
Handa, K., Hakomori, S.I.: Carbohydrate to carbohydrate interaction in development process and cancer progression. Glycoconj. J. (2012). doi:10.1007/s10719-012-9380-7
Watanabe, R., Ohyama, C., Aoki, H., Takahashi, T., Satoh, M., Saito, S., Hoshi, S., Ishii, A., Saito, M., Arai, Y.: Ganglioside G(M3) overexpression induces apoptosis and reduces malignant potential in murine bladder cancer. Cancer Res. 62(13), 3850–3854 (2002)
Prinetti, A., Aureli, M., Illuzzi, G., Prioni, S., Nocco, V., Scandroglio, F., Gagliano, N., Tredici, G., Rodriguez-Menendez, V., Chigorno, V., Sonnino, S.: GM3 synthase overexpression results in reduced cell motility and in caveolin-1 upregulation in human ovarian carcinoma cells. Glycobiology 20(1), 62–77 (2010). doi:10.1093/glycob/cwp143
Prinetti, A., Cao, T., Illuzzi, G., Prioni, S., Aureli, M., Gagliano, N., Tredici, G., Rodriguez-Menendez, V., Chigorno, V., Sonnino, S.: A glycosphingolipid/caveolin-1 signaling complex inhibits motility of human ovarian carcinoma cells. J. Biol. Chem. 286(47), 40900–40910 (2011). doi:10.1074/jbc.M111.286146
Nojiri, H., Yamana, H., Shirouzu, G., Suzuki, T., Isono, H.: Glycotherapy for cancer: remodeling of ganglioside pattern as an effective approach for cancer therapy. Cancer Detect. Prev. 26(2), 114–120 (2002)
Tringali, C., Lupo, B., Cirillo, F., Papini, N., Anastasia, L., Lamorte, G., Colombi, P., Bresciani, R., Monti, E., Tettamanti, G., Venerando, B.: Silencing of membrane-associated sialidase Neu3 diminishes apoptosis resistance and triggers megakaryocytic differentiation of chronic myeloid leukemic cells K562 through the increase of ganglioside GM3. Cell Death Differ. 16(1), 164–174 (2009). doi:10.1038/cdd.2008.141
Prinetti, A., Loberto, N., Chigorno, V., Sonnino, S.: Glycosphingolipid behaviour in complex membranes. Biochim. Biophys. Acta 1788(1), 184–193 (2009). doi:10.1016/j.bbamem.2008.09.001
Gabri, M.R., Otero, L.L., Gomez, D.E., Alonso, D.F.: Exogenous incorporation of neugc-rich mucin augments n-glycolyl sialic acid content and promotes malignant phenotype in mouse tumor cell lines. J. Exp. Clin. Cancer Res. 28, 146 (2009). doi:10.1186/1756-9966-28-146
Segatori, V.I., Vazquez, A.M., Gomez, D.E., Gabri, M.R., Alonso, D.F.: Preclinical evaluation of racotumomab, an anti-idiotype monoclonal antibody to N-glycolyl-containing gangliosides, with or without chemotherapy in a mouse model of non-small cell lung cancer. Front. Oncol. 2, 160 (2012). doi:10.3389/fonc.2012.00160
Segatori, V.I., Otero, L.L., Fernandez, L.E., Gomez, D.E., Alonso, D.F., Gabri, M.R.: Antitumor protection by NGcGM3/VSSP vaccine against transfected B16 mouse melanoma cells overexpressing N-glycolylated gangliosides. In Vivo 26(4), 609–617 (2012)
Labrada, M., Clavell, M., Bebelagua, Y., Leon, J., Alonso, D.F., Gabri, M.R., Veloso, R.C., Verez, V., Fernandez, L.E.: Direct validation of NGcGM3 ganglioside as a new target for cancer immunotherapy. Expert. Opin. Biol. Ther. 10(2), 153–162 (2010). doi:10.1517/14712590903443084
Miura, Y., Kainuma, M., Jiang, H., Velasco, H., Vogt, P.K., Hakomori, S.: Reversion of the Jun-induced oncogenic phenotype by enhanced synthesis of sialosyllactosylceramide (GM3 ganglioside). Proc. Natl. Acad. Sci. U. S. A. 101(46), 16204–16209 (2004). doi:10.1073/pnas.0407297101
Liu, J.W., Sun, P., Yan, Q., Paller, A.S., Gerami, P., Ho, N., Vashi, N., Le Poole, I.C., Wang, X.Q.: De-N-acetyl GM3 promotes melanoma cell migration and invasion through urokinase plasminogen activator receptor signaling-dependent MMP-2 activation. Cancer Res. 69(22), 8662–8669 (2009). doi:10.1158/0008-5472.CAN-09-1099
Hanahan, D., Weinberg, R.A.: Hallmarks of cancer: the next generation. Cell 144(5), 646–674 (2011). doi:10.1016/j.cell.2011.02.013
Birkle, S., Zeng, G., Gao, L., Yu, R.K., Aubry, J.: Role of tumor-associated gangliosides in cancer progression. Biochimie 85(3–4), 455–463 (2003)
Potapenko, M., Shurin, G.V., de Leon, J.: Gangliosides as immunomodulators. Adv. Exp. Med. Biol. 601, 195–203 (2007)
de Leon, J., Fernandez, A., Clavell, M., Labrada, M., Bebelagua, Y., Mesa, C., Fernandez, L.E.: Differential influence of the tumour-specific non-human sialic acid containing GM3 ganglioside on CD4 + CD25- effector and naturally occurring CD4 + CD25+ regulatory T cells function. Int. Immunol. 20(4), 591–600 (2008). doi:10.1093/intimm/dxn018
Sorice, M., Pavan, A., Misasi, R., Sansolini, T., Garofalo, T., Lenti, L., Pontieri, G.M., Frati, L., Torrisi, M.R.: Monosialoganglioside GM3 induces CD4 internalization in human peripheral blood T lymphocytes. Scand. J. Immunol. 41(2), 148–156 (1995)
Garofalo, T., Sorice, M., Misasi, R., Cinque, B., Giammatteo, M., Pontieri, G.M., Cifone, M.G., Pavan, A.: A novel mechanism of CD4 down-modulation induced by monosialoganglioside GM3. Involvement of serine phosphorylation and protein kinase c delta translocation. J. Biol. Chem. 273(52), 35153–35160 (1998)
Shurin, G.V., Shurin, M.R., Bykovskaia, S., Shogan, J., Lotze, M.T., Barksdale Jr., E.M.: Neuroblastoma-derived gangliosides inhibit dendritic cell generation and function. Cancer Res. 61(1), 363–369 (2001)
Peguet-Navarro, J., Sportouch, M., Popa, I., Berthier, O., Schmitt, D., Portoukalian, J.: Gangliosides from human melanoma tumors impair dendritic cell differentiation from monocytes and induce their apoptosis. J. Immunol. 170(7), 3488–3494 (2003)
Padler-Karavani, V., Yu, H., Cao, H., Chokhawala, H., Karp, F., Varki, N., Chen, X., Varki, A.: Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: potential implications for disease. Glycobiology 18(10), 818–830 (2008). doi:10.1093/glycob/cwn072
Taylor, R.E., Gregg, C.J., Padler-Karavani, V., Ghaderi, D., Yu, H., Huang, S., Sorensen, R.U., Chen, X., Inostroza, J., Nizet, V., Varki, A.: Novel mechanism for the generation of human xeno-autoantibodies against the nonhuman sialic acid N-glycolylneuraminic acid. J. Exp. Med. 207(8), 1637–1646 (2010). doi:10.1084/jem.20100575
Hedlund, M., Padler-Karavani, V., Varki, N.M., Varki, A.: Evidence for a human-specific mechanism for diet and antibody-mediated inflammation in carcinoma progression. Proc. Natl. Acad. Sci. U. S. A. 105(48), 18936–18941 (2008). doi:10.1073/pnas.0803943105
Fuji, H., Iribe, H.: Clonal variation in tumorigenicity of L1210 lymphoma cells: nontumorigenic variants with an enhanced expression of tumor-associated antigen and Ia antigens. Cancer Res. 46(11), 5541–5547 (1986)
Acknowledgments
We thank Dr. Luis Enrique Fernández for his valuable comments on the project. We are also grateful to our fellow researchers Alex Miranda, Darel Martínez, Mayrel Labrada, Nely Rodríguez and Drs. Ana María Hernández and Adriana Carr, as well as technicians Judith Raymond and Katya Sosa for their help. This work was supported by the Center of Molecular Immunology.
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Ana Victoria Casadesús and Yuniel Fernández-Marrero contributed equally to this work
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Casadesús, A.V., Fernández-Marrero, Y., Clavell, M. et al. A shift from N-glycolyl- to N-acetyl-sialic acid in the GM3 ganglioside impairs tumor development in mouse lymphocytic leukemia cells. Glycoconj J 30, 687–699 (2013). https://doi.org/10.1007/s10719-013-9473-y
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DOI: https://doi.org/10.1007/s10719-013-9473-y