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The Effect of Saccharomyces boulardii on Human Colon Cells and Inflammation in Rats with Trinitrobenzene Sulfonic Acid-Induced Colitis

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Abstract

Saccharomyces boulardii (S. boulardii) has beneficial effects in the treatment of intestinal inflammation; however, little is known about the mechanisms by which these effects occur. We investigated the effects of S. boulardii on the expression of peroxisome proliferator-activated receptor-gamma (PPAR-γ) and interleukin-8 (IL-8), using human HT-29 colonocytes and a rat model of trinitrobenzene sulfonic acid (TNBS)-induced colitis. The effect of S. boulardii on gene expression was assessed by semi-quantitative reverse transcription–polymerase chain reaction (RT-PCR), and Northern blot and Western blot assays. Pharmacological inhibitors for various signaling pathways were used to determine the signaling pathways implicated in the S. boulardii regulation of PPAR-γ and IL-8. We found that S. boulardii up-regulated and down-regulated PPAR-γ and IL-8 expression at the transcription level, both in vitro and in vivo (P < 0.05, respectively). Saccharomyces boulardii blocked tumor necrosis factor-alpha (TNF-α) regulation of PPAR-γ and IL-8 through disruption of TNF-α-mediated nuclear factor kappa B (NF-κB) activation. Furthermore, S. boulardii suppressed colitis and expression of pro-inflammatory cytokine genes in vivo (P < 0.05, respectively). Our study demonstrated that S. boulardii reduces colonic inflammation and regulates inflammatory gene expression.

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Acknowledgments

This work was supported by grants from the Korea Research Foundation (KRF-2005-003-E00084) and the Korea Science and Engineering Foundation (the biofood R&D Program).

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Authors and Affiliations

  1. Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Korea

    Sang Kil Lee

  2. Department of Pathology, School of Medicine, Kyung Hee University, Seoul, Korea

    Youn Wha Kim

  3. School of Life Sciences and Biotechnology, Korea University, 5-1 Anam-dong, Sungbuk-gu, Seoul, 136-701, Korea

    Sung-Gil Chi & Yeong-Shil Joo

  4. Department of Internal Medicine, School of Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-752, Korea

    Hyo Jong Kim

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  1. Sang Kil Lee
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Correspondence to Sung-Gil Chi or Hyo Jong Kim.

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Lee, S.K., Kim, Y.W., Chi, SG. et al. The Effect of Saccharomyces boulardii on Human Colon Cells and Inflammation in Rats with Trinitrobenzene Sulfonic Acid-Induced Colitis. Dig Dis Sci 54, 255–263 (2009). https://doi.org/10.1007/s10620-008-0357-0

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