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Preventative effects of Lactobacillus plantarum YS-3 on oxazolone-induced BALB/c colitis in mice

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Abstract

In this study, the preventative effects of Lactobacillus plantarum YS-3 (LP-YS3) on colitis were studied using an in vitro animal experiment. Oxazolone was administered to BALB/c mice to induce colitis, and the preventive effects of LP-YS3 were determined using serum- and tissue-related indexes. The mice treated with LP-YS3 showed a significant decrease (p < 0.05) in disease activity index for colitis by inhibiting colon shortening and increasing colon weight/length ratio. The application of LP-YS3 resulted in a significant reduction in myeloperoxidase, nitric oxide, and malondialdehyde activities and a decrease in glutathione activity (p < 0.05) in mouse colon tissues. LP-YS3 also significantly increased serum interleukin-2 and reduced interleukin-10 cytokines levels in mice (p < 0.05). Reverse transcription-polymerase chain reaction and western blot assays showed that LP-YS3 application resulted in a significant increase in neuronal nitric oxide synthase, endothelial nitric oxide synthase, c-Kit, and stem cell factor expressions and a decrease in inducible nitric oxide synthase, interleukin-8, and C-X-C motif chemokine receptor 2 expressions in mouse colonic tissues (p < 0.05). These findings indicate that LP-YS3 imparts preventative effects on oxazolone-induced colitis in mice.

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Acknowledgments

The present research was supported by the Program for Innovation Team Building at Institutions of Higher Education in Chongqing (CXTDX201601040), Chongqing Research Program of Basic Research and Frontier Technology (cstc2016jcyjA0339) and Research Project of Chongqing University of Education (KY2015TBZC), China.

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  1. Xia Feng and Jing Zhang have contributed equally to this work.

Authors and Affiliations

  1. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Main Street 9, Nan’an District, Chongqing, 400067, People’s Republic of China

    Xia Feng, Yu Qian, Ruokun Yi, Peng Sun, Jianfei Mu & Xin Zhao

  2. Department of Environmental and Quality Inspection, Chongqing Chemical Industry Vocational College, Chongqing, People’s Republic of China

    Jing Zhang

  3. Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Huancheng North 2 Road 109, Guilin, 541004, Guangxi, People’s Republic of China

    Jia-Le Song

Authors
  1. Xia Feng
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  2. Jing Zhang
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  3. Yu Qian
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  4. Ruokun Yi
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  5. Peng Sun
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  7. Xin Zhao
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Correspondence to Xin Zhao or Jia-Le Song.

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Feng, X., Zhang, J., Qian, Y. et al. Preventative effects of Lactobacillus plantarum YS-3 on oxazolone-induced BALB/c colitis in mice. Appl Biol Chem 61, 355–363 (2018). https://doi.org/10.1007/s13765-018-0359-3

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