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Chromosome localization of microsatellite markers in the shrews of the Sorex araneus group

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Abstract

The extremely high rate of karyotypic evolution that characterizes the shrews of the Sorex araneus group makes this group an exceptionally interesting model for population genetics and evolutionary studies. Here, we attempted to map 46 microsatellite markers at the chromosome arm level using flow-sorted chromosomes from three karyotypically different taxa of the Sorex araneus group (S. granarius and the chromosome races Cordon and Novosibirsk of S. araneus). The most likely localizations were provided for 35 markers, among which 25 were each unambiguously mapped to a single locus on the corresponding chromosomes in the three taxa, covering the three sexual chromosomes (XY1Y2) and nine of the 18 autosomal arms of the S. araneus group. The results provide further evidence for a high degree of conservation in genome organization in the S. araneus group despite the presence of numerous Robertsonian rearrangements. These markers can therefore be used to compare the genetic structure among taxa of the S. araneus group at the chromosome level and to study the role of chromosomal rearrangements in the genetic diversification and speciation process of this group.

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Authors and Affiliations

  1. Department of Ecology and Evolution, Lausanne University, Biophore, 1015, Lausanne, Switzerland

    Patrick Basset, Glenn Yannic & Jacques Hausser

  2. Centre for Veterinary Science, University of Cambridge, Cambridge, CB2 1TN, UK

    Fengtang Yang, Patricia C. M. O'Brien, Malcolm A. Ferguson-Smith & Gabriel Balmus

  3. Russian Academy of Science, Institute of Cytology and Genetics, Novosibirsk, 630090, Russia

    Alexander S. Graphodatsky

  4. Museum of Natural History, National Laboratory, Origin Struct & Evolut Biodivers, 55 Rue Buffon, Paris, 75005, France

    Vitaly T. Volobouev

  5. Faculty of Veterinary Medicine, Iasi, 700489, Romania

    Gabriel Balmus

  6. Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK

    Fengtang Yang

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  1. Patrick Basset
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  2. Glenn Yannic
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  3. Fengtang Yang
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  4. Patricia C. M. O'Brien
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  5. Alexander S. Graphodatsky
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  6. Malcolm A. Ferguson-Smith
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  7. Gabriel Balmus
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  8. Vitaly T. Volobouev
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  9. Jacques Hausser
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Correspondence to Patrick Basset.

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Basset, P., Yannic, G., Yang, F. et al. Chromosome localization of microsatellite markers in the shrews of the Sorex araneus group. Chromosome Res 14, 253–262 (2006). https://doi.org/10.1007/s10577-006-1041-x

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  • DOI: https://doi.org/10.1007/s10577-006-1041-x

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