Abstract
Polyploidy is a phenomenon that alters the genetic diversity of populations and has been reported as one of the most important evolutionary forces for plant diversification. The Psidium cattleyanum complex comprises a group of wild populations with several ploidy levels reported in the literature. The multiple cytotypes, associated with its wide distribution area, make this species a potential key model for understanding evolutionary processes related to polyploidization. In this study, we isolated and characterized nuclear microsatellite markers of P. cattleyanum and tested their transferability to other nine species of the genus. We performed a preliminary analysis of genetic diversity and population structure in three populations of P. cattleyanum. The three populations analyzed had different chromosome numbers, being polyploid cytotypes (2n = 6x = 66, 2n = 7x = 77 and 2n = 8x = 88). We designed 46 primer pairs and successfully amplified 37 markers, from which the 10 best were selected for analysis. Considering both the PIC and DP values, most of markers were highly informative. The new SSR markers were used to assess the levels of genetic diversity of the populations and detected one population with predominance of sexual reproduction. DAPC analysis pointed the formation of three groups, which corresponded to the populations analyzed. The markers were successfully amplified in related species, with some species presenting 80% transferability. By producing this panel of polymorphic microsatellites, we contribute to the understanding evolution in groups of natural polyploids for future studies.
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Acknowledgements
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001. We thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and FAEPEX/UNICAMP for the financial support. FAO received a Post-Doctoral fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2018/18527-9). We thank Prof. Marcia Dr. Flores da Silva Ferreira, of Universidade Federal do Espírito Santo, for sending the different Psidium species plant material, and Dr. André Vito Scatigna for his support in fieldwork and collecting plant material from the individual grown in Uruguaiana (RS). We also thank the staff of Laboratório de Análises Genéticas e Moleculares (LAGM/UNICAMP).
Funding
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and FAEPEX/UNICAMP. FAO received a Post-Doctoral fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2018/18527-9).
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Machado, R.M., de Oliveira, F.A., de Matos Alves, F. et al. Population Genetics of Polyploid Complex Psidium cattleyanum Sabine (Myrtaceae): Preliminary Analyses Based on New Species-Specific Microsatellite Loci and Extension to Other Species of the Genus. Biochem Genet 59, 219–234 (2021). https://doi.org/10.1007/s10528-020-10002-1
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DOI: https://doi.org/10.1007/s10528-020-10002-1