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Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity

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Abstract

Ionizing radiation generates oxidative stress, which is thought to be a major cause of aging. Although living organisms are constantly exposed to low levels of radiation, most studies examining the effect of radiation have focused on accelerated aging and diminished life span that result from high-dose radiation. On the other hand, several studies have suggested that low-dose radiation enhances the longevity of Drosophila melanogaster. Therefore, investigation of the biological effects of low-dose radiation could contribute to a more comprehensive understanding of the aging process. In this study, microarray and quantitative real time-PCR were used to measure genome-wide changes in transcript levels in low-dose irradiated fruit flies that showed enhanced longevity. In response to radiation, approximately 13% of the genome exhibited changes in gene expression, and a number of aging-related genes were significantly regulated. These data were compared with quantitative trait loci affecting life-span to identify candidate genes involved in enhanced longevity induced by low-dose radiation. This genome-wide survey revealed novel information about changes in transcript levels in low-dose irradiated flies and identified 39 new candidate genes for molecular markers of extended longevity induced by ionizing radiation. In addition, this study also suggests a mechanism by which low-dose radiation extends longevity.

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Acknowledgments

This work was supported by grants from the Korea Hydro and Nuclear Power Co., Ltd., A08NJ23 and the Ministry of Education, Science and Technology (MEST) and National Research Foundation of Korea (NRF) through Nuclear R&D Program, 2009 (No. 39584).

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Authors and Affiliations

  1. Division of Radiation Effect Research, Radiation Health Research Institute, Korea Hydro & Nuclear Power Co., Ltd., 388-1 Ssangmun 3-Dong, Dobong-Gu, Seoul, 132-703, Korea

    Ki Moon Seong, Cha Soon Kim, Sun-Won Seo, Ha Yeon Jeon, Seon Young Nam, Kwang Hee Yang, Ji-Young Kim & Young-Woo Jin

  2. Department of Biological Sciences, Inha University, Incheon, 402-751, Korea

    Byung-Sub Lee & Kyung-Jin Min

  3. Department of Nuclear Medicine, Haeundae Paik Hospital, Inje University, Busan, 612-030, Korea

    Chong Soon Kim

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  1. Ki Moon Seong
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Correspondence to Young-Woo Jin.

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Ki Moon Seong and Cha Soon Kim contributed equally to this work.

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Seong, K.M., Kim, C.S., Seo, SW. et al. Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity. Biogerontology 12, 93–107 (2011). https://doi.org/10.1007/s10522-010-9295-2

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