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Isoflavone aglycones production from isoflavone glycosides by display of β-glucosidase from Aspergillus oryzae on yeast cell surface

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Abstract

For efficient production of isoflavone aglycones from soybean isoflavones, we isolated three novel types of β-glucosidase (BGL1, BGL3, and BGL5) from the filamentous fungi Aspergillus oryzae. Three enzymes were independently displayed on the cell surface of a yeast Saccharomyces cerevisiae as a fusion protein with α-agglutinin. Three β-glucosidase-displaying yeast strains hydrolyzed isoflavone glycosides efficiently but exhibited different substrate specificities. Among these β-glucosidases, BGL1 exhibited the highest activity and also broad substrate specificity to isoflavone glycosides. Although glucose released from isoflavone glycosides are generally known to inhibit β-glucosidase, the residual ratio of isoflavone glycosides in the reaction mixture with BGL1-displaying yeast strain (Sc-BGL1) reached approximately 6.2%, and the glucose concentration in the reaction mixture was maintained at lower level. This result indicated that Sc-BGL1 assimilated the glucose before they inhibited the hydrolysis reaction, and efficient production of isoflavone aglycones was achieved by engineered yeast cells displaying β-glucosidase.

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Acknowledgement

This work was financed by Research and Development Program for New Bio-industry Initiatives.

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Authors and Affiliations

  1. Research Institute, Gekkeikan Sake Co., Ltd., 101 Shimotoba-koyanagi-cho, Fushimi-ku, Kyoto, 612-8385, Japan

    Masahiko Kaya, Atsushi Kotaka, Kengo Matsumura, Hiroki Bando, Hiroshi Sahara & Yoji Hata

  2. Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan

    Junji Ito, Chiaki Ogino & Akihiko Kondo

  3. Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, Minatojima, Chuo-ku, Kobe, 650-8530, Japan

    Seiji Shibasaki

  4. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake, Sakyo-ku, Kyoto, 606-8502, Japan

    Kouichi Kuroda & Mitsuyoshi Ueda

Authors
  1. Masahiko Kaya
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  2. Junji Ito
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  3. Atsushi Kotaka
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  4. Kengo Matsumura
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  5. Hiroki Bando
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  6. Hiroshi Sahara
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  7. Chiaki Ogino
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  8. Seiji Shibasaki
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  9. Kouichi Kuroda
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  10. Mitsuyoshi Ueda
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  11. Akihiko Kondo
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  12. Yoji Hata
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Corresponding author

Correspondence to Yoji Hata.

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Kaya, M., Ito, J., Kotaka, A. et al. Isoflavone aglycones production from isoflavone glycosides by display of β-glucosidase from Aspergillus oryzae on yeast cell surface. Appl Microbiol Biotechnol 79, 51–60 (2008). https://doi.org/10.1007/s00253-008-1393-6

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  • DOI: https://doi.org/10.1007/s00253-008-1393-6

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