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Cloning and functional expression of thermostable β-glucosidase gene from Thermoascus aurantiacus

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Abstract

A thermostable β-glucosidase (BGLI) was purified from Thermoascus aurantiacus IFO9748, and the gene (bgl1) encoding this enzyme was cloned and expressed in yeast Pichia pastoris. The deduced amino acid sequence encoded by bgl1 showed high similarity with the sequence of glycoside hydrolase family 3. The recombinant enzyme was purified and subjected to enzymatic characterization. Recombinant BGLI retained more than 70% of its initial activity after 1 h of incubation at 60°C and was stable in the pH range 3–8. The optimal temperature for enzyme activity was about 70°C and the optimal pH was about 5. P. pastoris expressing recombinant BGLI became able to utilize cellobiose as a carbon source.

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Acknowledgments

This work was supported by grants-in-aid for scientific research from the Ministry of Education, Science, Sports and Culture of Japan; the New Energy and Industrial Technology Development Organization; Japan Society for the Promotion of Science; and the Research Institute of Innovative Technology for the Earth.

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Authors and Affiliations

  1. Laboratory of Applied Microbiology, Research Institute of Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi-cho, Ishikawa, 921-8836, Japan

    Jiong Hong & Hidehiko Kumagai

  2. Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Hisanori Tamaki

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  1. Jiong Hong
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  2. Hisanori Tamaki
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Correspondence to Hisanori Tamaki.

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Hong, J., Tamaki, H. & Kumagai, H. Cloning and functional expression of thermostable β-glucosidase gene from Thermoascus aurantiacus . Appl Microbiol Biotechnol 73, 1331–1339 (2007). https://doi.org/10.1007/s00253-006-0618-9

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