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Cloning and chromosomal location of human genes inducible by type I interferon

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Somatic Cell and Molecular Genetics

Abstract

When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-α, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose or of time. The IFI-4 and IFI-54K genes, as well as two previously isolated IFN-inducible genes, namely the IFI-56K and low-molecular-weight 2–5A synthetase, were localized on the human chromosomes. Using cloned probes on Southern blots of DNA from a panel of rodent-human somatic cell hybrids, we have assigned the IFI-4 gene to chromosome 1 and the gene coding for the low-molecular-weight 2–5A synthetase to chromosome 12. We also showed that the IFI-54K and IFI-56K genes, unlike most of the IFN-inducible genes, are syntenic. They are both located on chromosome 10. In addition, evidence is given for the presence of a pseudogene homologous to IFI-56K on chromosome 13.

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Authors and Affiliations

  1. Département de Biologie Moléculaire, Université Libre de Bruxelles, 67 rue des Chevaux, B-1640, Rhode-St-Genèse, Belgium

    Marc G. Wathelet, Josiane Szpirer, Christine B. Nols, Isabelle M. Clauss, Claude Szpirer & Georges A. Huez

  2. Institut Pasteur du Brabant, 642 rue Engeland, B-1180, Bruxelles, Belgium

    Lukas De Wit & Jean Content

  3. Department of Genetics, University of Göteborg, S40033, Göteborg, Sweden

    M. Quamrul Islam & Göran Levan

  4. Pharmaceutical Research, Ciba-Geigy Ltd., CH-4002, Basle, Switzerland

    Michel A. Horisberger

Authors
  1. Marc G. Wathelet
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  2. Josiane Szpirer
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  3. Christine B. Nols
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  4. Isabelle M. Clauss
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  5. Lukas De Wit
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  6. M. Quamrul Islam
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  8. Michel A. Horisberger
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  9. Jean Content
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  10. Claude Szpirer
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  11. Georges A. Huez
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Wathelet, M.G., Szpirer, J., Nols, C.B. et al. Cloning and chromosomal location of human genes inducible by type I interferon. Somat Cell Mol Genet 14, 415–426 (1988). https://doi.org/10.1007/BF01534709

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  • DOI: https://doi.org/10.1007/BF01534709

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