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Simultaneous Isolation and Purification of Mollugin and Two Anthraquinones from Rubia cordifolia by HSCCC

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Abstract

A preparative high-speed countercurrent chromatography (HSCCC) method for isolation and purification of three bioactive components, tectoquinone; 1-hydroxy-2-methylanthraquinone and mollugin from the Chinese medicinal plant Rubia cordifolia was successfully established. With a two-phase solvent system composed of light petroleum/ethanol/diethyl ether/water (5:4:3:1, v/v), 10 mg tectoquinone, 19 mg 1-hydroxy-2-methylanthraquinone and 16 mg mollugin were obtained from 100 mg of the crude petroleum extract in a one-step separation at a purity of 98.8, 95.8 and 98.3%, respectively. The structures of mollugin and anthraquinones were identified by 1H NMR and 13C NMR.

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Acknowledgment

Financial support from the Natural Science Foundation of Zhejiang Province (Z206510) and the Zhejiang Provincial Science & Technology Department Project (2006C12098) are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Zhejiang University, 310027, Hangzhou, China

    Rui Liu, Yanbin Lu, Tianxing Wu & Yuanjiang Pan

Authors
  1. Rui Liu
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  2. Yanbin Lu
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  3. Tianxing Wu
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  4. Yuanjiang Pan
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Correspondence to Tianxing Wu or Yuanjiang Pan.

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Liu, R., Lu, Y., Wu, T. et al. Simultaneous Isolation and Purification of Mollugin and Two Anthraquinones from Rubia cordifolia by HSCCC. Chroma 68, 95–99 (2008). https://doi.org/10.1365/s10337-008-0629-z

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  • DOI: https://doi.org/10.1365/s10337-008-0629-z

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