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Recombinant Analogue of the Human Protein SLURP-1 Inhibits the Growth of U251 MG and A172 Glioma Cells

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

The alpha7 nicotinic acetylcholine receptor (α7-nAChR) is considered a promising pharmacological target for the carcinoma therapy. We have previously shown that the recombinant analogue of the human protein SLURP-1 (rSLURP-1) effectively inhibits the growth of carcinomas of various origins via the interaction with α7-nAChR and down-regulation of expression of this receptor. Expression of α7-nAChR is increased in gliomas compared to healthy human brain tissues; however, the role of this receptor in the gliomas development is poorly understood. It was shown for the first time that rSLURP-1 significantly inhibits the growth of glioma model cells U251 MG and A172 up to ∼70%, which is comparable with the effect of α-bungarotoxin, a selective α7-nAChR inhibitor. The half-maximum effective concentrations of rSLURP-1 for U251 MG and A172 cells were 2.82 ± 0.2 and 8.9 ± 0.3 nM, respectively. Coincubation of U251 MG cells with rSLURP-1 and the nAChR inhibitor mecamylamine attenuates the antiproliferative activity of rSLURP-1, indicating nAChR as a molecular target for the rSLURP-1 action in gliomas.

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Funding

This work was supported by the Russian Science Foundation (project no. 17-74-20161).

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    M. A. Shulepko, M. L. Bychkov, E. N. Lyukmanova &  M. P. Kirpichnikov

  2. Biological Faculty, Moscow State University, Moscow, Russia

    M. P. Kirpichnikov

Authors
  1. M. A. Shulepko
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  2. M. L. Bychkov
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  3. E. N. Lyukmanova
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  4. M. P. Kirpichnikov
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Corresponding author

Correspondence to E. N. Lyukmanova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Shulepko, M.A., Bychkov, M.L., Lyukmanova, E.N. et al. Recombinant Analogue of the Human Protein SLURP-1 Inhibits the Growth of U251 MG and A172 Glioma Cells. Dokl Biochem Biophys 493, 211–214 (2020). https://doi.org/10.1134/S1607672920040134

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  • DOI: https://doi.org/10.1134/S1607672920040134

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