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Meiotic Chromosomes, Synaptonemal Complexes in a Female Viviparous Lizard (Zootoca vivipara) in Prophase I of Meiosis

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Abstract

In the females of the viviparous lizard Zootoca vivipara (Lichtenstein, 1823) (family Lacertidae) from Northwest Russia (2n = 35: 32A (acrocentric autosomes) + Z1Z2W sex chromosomes), the ovarian lumen germinal vesicles (oocytes), as well as germinal lamina cells, were examined. Chromosome preparations were obtained using the direct method and the method of total oocyte nuclei spreading developed by Dresser and Moses. Chromosome preparations were stained with Giemsa; for visualization of synaptonemal complexes (SCs), total preparations of oocyte nuclei were stained with silver nitrate and DAPI. It was demonstrated that, during oogenesis in the female, primary follicles enter the early stages of the meiotic prophase I (stages from leptotene to diplotene, lampbrush chromosomes are formed). Here, for the first time, total oocyte spreads were obtained and studied. On the basis of light microscopic analysis of the oocyte SCs and taking into account their length in a female with 2n = 35, the SC karyotype is presented, consisting of 19 SC elements, among which 16 SC autosomal bivalents are distinguished. The remaining three SC elements, according to the authors, can be univalents of Z1Z2W sex chromosomes or one WZ1 bivalent and Z2 and B chromosome univalents. As in the SC karyotype of Z. vivipara males, in a female, specific features in the morphology of SC elements of chromosomes 5 and 6 were observed.

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ACKNOWLEDGMENTS

We thank O.L. Kolomiets, I.N. Golubovskaya, and V.E. Spangenberg for the attention to the study and valuable advice.

Funding

This study was supported by grant no. AAAA-A19-119020590095-9.

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Authors and Affiliations

  1. Zoological Institute, Russian Academy of Sciences, 199034, St. Petersburg, Russia

    L. A. Kupriyanova

  2. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, 119071, Moscow, Russia

    L. D. Safronova

  3. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    A. I. Chekunova

Authors
  1. L. A. Kupriyanova
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  2. L. D. Safronova
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  3. A. I. Chekunova
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Correspondence to L. A. Kupriyanova.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by N. Maleeva

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Kupriyanova, L.A., Safronova, L.D. & Chekunova, A.I. Meiotic Chromosomes, Synaptonemal Complexes in a Female Viviparous Lizard (Zootoca vivipara) in Prophase I of Meiosis. Russ J Genet 55, 774–778 (2019). https://doi.org/10.1134/S1022795419060085

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