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In vitro response of transgenic aspen containing glutamine synthetase gene GSI to the sublethal dose of phosphinothricin

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Abstract

Transgenic aspen plants containing the glutamine synthetase gene GSI from pine have been produced. Among 37 transformed lines, 34 were found to possess GSI. The RT-PCR analysis of GSI transcripts confirmed the presence of specific transcripts in 32 lines. The phenotypic effect of a glutamine synthetase activity in transgenic plants was evaluated by in vitro cultivation of plants at the presence of a sublethal dose (0.5 mg/l) of phosphinothricin, which inhibits this enzyme. It was shown that the sublethal dose of this herbicide provides a predictable inhibiting effect on the nontransformed aspen plants, including the inhibition of their rhizogenesis, whereas transgenic plants demonstrated various responses. In most transgenic lines, we observed an unexpected stimulating effect of low herbicide doses on in vitro rhizogenesis; this effect was manifested through the increased radication frequency, increased average number of roots per plant, and increased total length of roots.

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Abbreviations

DMSO:

dimethyl sulphoxide

DTT:

dithiothreitol

RI:

response index

IAA:

indoleacetic acid

RT-PCR:

PCR with the use of reverse transcriptase

bp:

(number) of base pairs

MS medium:

Murashige and Skoog nutrient medium

CTAB:

N-cetyl-N,N,N-trimethylammonium bromide

GS:

glutamine synthetase

PPT:

phosphinotricin

WPM:

woody plant medium

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Authors and Affiliations

  1. Pushchino Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, 142290, Russia

    K. A. Shestibratov, I. V. Bulatova & P. S. Novikov

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  1. K. A. Shestibratov
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  2. I. V. Bulatova
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  3. P. S. Novikov
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Correspondence to K. A. Shestibratov.

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Original Russian Text © K.A. Shestibratov, I.V. Bulatova, P.S. Novikov, 2009, published in Biotekhnologiya, 2009, No. 6, pp. 57–64.

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Shestibratov, K.A., Bulatova, I.V. & Novikov, P.S. In vitro response of transgenic aspen containing glutamine synthetase gene GSI to the sublethal dose of phosphinothricin. Appl Biochem Microbiol 46, 763–768 (2010). https://doi.org/10.1134/S0003683810080053

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