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Active conformation of a-chymotrypsin in organic solvents as studied by circular dichroism

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Biotechnology Techniques

Abstract

Circular dichroic (CD) spectra of a-chymotrypsin were measured in mixtures of water and ethanol, acetonitrile, or tetrahydrofuran. The catalytic activity of a-chymotrypsin in the different solvents was related to the change of CD bands especially that at 230 nm. The fine structure in near-UV region may also be useful to estimate the catalytic activity of a-chymotrypsin.

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Authors and Affiliations

  1. Institute of Materials Science, University of Tsukuba, Tennoudai, Tsukuba Ibaraki, 305, Japan

    Toshiya Sasaki, Masami Kobayashi & Hideo Kise

Authors
  1. Toshiya Sasaki
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  2. Masami Kobayashi
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  3. Hideo Kise
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Sasaki, T., Kobayashi, M. & Kise, H. Active conformation of a-chymotrypsin in organic solvents as studied by circular dichroism. Biotechnology Techniques 11, 387–390 (1997). https://doi.org/10.1023/A:1018456420407

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