Skip to main content
SpringerLink
Log in

Exploration of mangrove-associated actinobacteria from South Andaman Islands, India

  • Original Article
  • Published:
Systems Microbiology and Biomanufacturing Aims and scope Submit manuscript

Abstract

In this study, a total of 1125 actinobacteria were isolated from the selected mangrove species: Avicennia marina, Rhizopora mucronata and Ceriops tagal from three study stations viz., Minnie Bay, Carbyn’s Cove and Burmanallah. Among these three stations, the highest number of actinobacteria was recorded in Carbyn’s Cove (64.97%), followed by (25.51%) at Burmanallah and the minimum of (9.51%) was recorded in Minnie Bay. Maximum number of actinobacteria was recorded from Ceriops tagal (40.44%) than the other selected mangrove species Avicennia marina (34.13%) and Rhizopora mucronata (25.42%). Among the 1,125 mangrove-associated actinobacteria, 103 morphologically different isolates from the Minnie Bay station was selected for the further characterization studies. In antibacterial assay, 30.11% of the isolates revealed inhibitory activity against all tested clinical pathogens and 65% isolates displayed inhibitory activity against minimum of 04 tested clinical pathogens. Growth survival studies of the actinobacterial isolates also accomplished to withstand in varied NaCl and pH levels. Of 103 isolates, all were found to synthesize gelatinase enzyme, 73 isolates demonstrated amylolytic activity, 38 isolates exhibited proteolytic and 63 isolates displayed urease activity. Interestingly, 56 isolates exhibited excellent DNase activity and 71 isolates revealed positive for l-asparaginase production. To our recognition, 11 isolates exhibited constructive results in the production of 06 extracellular enzymes of industrial importance. Of 103 isolates, 48 isolates were confirmed by molecular level identification. Based on the phylogenetic analysis, the isolates were categorized under the genera: Streptomyces, Nocardiopsis, Salinispora and Actinomadura.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

Availability of data and materials

Not Applicable.

References

  1. Kathiresan NK, Bingham BL. Biology of mangroves and mangrove ecosystems. Adv Mar Biol. 2001;40:81–251.

    Article  Google Scholar 

  2. Brown BE. Integrated coastal management: South Asia. Newcastle, Newcastle upon Tyne: Department of Marine Sciences and Coastal Management, Univ; 1997.

    Google Scholar 

  3. Baskaran R, Vijayakumar R, Mohan PM. Enrichment method for the isolation of bioactive actinomycetes from mangrove sediments of Andaman islands, India. Malays J Microbiol. 2011;7:1–7.

    Google Scholar 

  4. Bredholt H, Fjaervik E, Jhonsen G, Zotechev SB. Actinomycetes from sediments in the Trondhein Fjrod, Norway: diversity and biological activity. Mar Drugs. 2008;6:12–24.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Pelaez F. The historical delivery of antibiotic from microbial natural products—can history repeat? Biochem Pharmacol. 2006;71:981–90.

    Article  CAS  PubMed  Google Scholar 

  6. Veiga M, Esparis A, Fabregas J. Isolation of cellulolytic actinomycetes from marine sediments. Appl Environ Microbiol. 1983;46:286–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Jensen PR, Gontang E, Mafnas C, Mincer TJ, Fenical W. Culturable marine actinomycetes diversity from tropical Pacific ocean sediments. Appl Environ Microbiol. 2005;7:1039–48.

    Google Scholar 

  8. Kumar KS, Sahu MK, Kathiresan K. Isolation and characterization of streptomycetes producing antibiotic, from a mangrove environment. Asian J Microbiol Biotechnol Environ Exp Sci. 2005;7:457–64.

    CAS  Google Scholar 

  9. Jeffrey LSH, SahilahSon AMR, Tosiah S. Isolation and screening of actinomycetes from Malaysian soil for their enzymatic and antimicrobial activities. J Tropical Agric Food Sci. 2007;35:159–64.

    Google Scholar 

  10. Oskay AM, Usame T, Cem A. Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African J Biotechol. 2004;3:441–6.

    Article  Google Scholar 

  11. Meena B, Anburajan L, Vinithkumar NV, Kirubagaran R. Novel marine actinobacteria from emerald Andaman & Nicobar islands: a prospective source for industrial and pharmaceutical byproducts. BMC Microbiol. 2013;13:145.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Ellaiah P, Kalyan D, Rao VS, Rao BV. Isolation and characterization of bioactive actinomycetes from marine sediments. Hindustan Antibiot Bull. 1996;38:48–52.

    CAS  PubMed  Google Scholar 

  13. Kuster E, Williams S. Selection of media for the isolation of Streptomyces. Nature. 1964;202:928–9.

    Article  Google Scholar 

  14. Weyland H. Actinomycetes in North sea and Atlantic ocean sediments. Nature. 1969;223:858.

    Article  CAS  PubMed  Google Scholar 

  15. Shirling EB, Gottileb D. Methods for characterization of Streptomyces species. Int J Syst Bactriol. 1966;16:312–40.

    Google Scholar 

  16. Pridham TG, Gottlieb D. The utilization of carbon compounds by some actinomycetes as an aid for species determination. J Bacteriol. 1948;56:107–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Williams ST, Cross T. Actinomycetes, methods in microbiology, vol. 4. New York: Academic Press; 1971.

    Google Scholar 

  18. Lemos ML, Toranzo AE, Barja JL. Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds. Microb Ecol. 1985;11:149–63.

    Article  CAS  PubMed  Google Scholar 

  19. Salle AJ. Laboratory manual on fundamental principles of bacteriology. UK: McGraw Hill; 1948.

    Google Scholar 

  20. Leon J, Liza L, Soto I, Cuadra D, Patino L, Zerpa R. Bioactive actinomycetes of marine sediment from the central coast of Peru. Rev Peru Biol. 2007;14:259–70.

    Article  Google Scholar 

  21. Gordon RE, Mihm JM. A comparative study of some strains received as Nocardiae. J Bacteriol. 1957;73:15–27.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Gulati R, Saxena RK, Gupta R. A rapid plate assay for screening L-asparaginase producing microorganisms. Lett Appl Microbiol. 1997;24:23–6.

    Article  CAS  PubMed  Google Scholar 

  23. Kutchama AJ, Roberts MA, Knaebel DB, Crawford DL. Small-scale isolation of genomic DNA from Streptomyces mycelia or spores. Biotechniques. 1998;24:452–6.

    Article  Google Scholar 

  24. Altschul SF, Thomas LM, Alejandro AS, Zhang J, Zhang Z, Miller W, Lipman DJ. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997;25:3389–402.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Talbot GH, Bradley J, Edwards JE Jr, Gilbert D, Scheld M, Bartlett JG. Bad bugs need drugs: an update on the development pipeline from the antimicrobial availability task force of the infectious diseases society of America. Clin Infect Dis. 2006;42:657–68.

    Article  PubMed  Google Scholar 

  27. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:783–9.

    Article  PubMed  Google Scholar 

  28. Vanajakumar G, Selvakumar N, Natarajan R. Antagonistic properties of actinomycetes isolated from mollusks of the porto Novo region. South India. 1995: 267–274.

  29. Saurav K, Kannabiran K. Diversity and optimization of process parameters for the growth of Streptomyces VITSVK9 sp. isolated from Bay of Bengal, India. J Nat Environ Sci. 2010;1:56–65.

    Google Scholar 

  30. Bentley SD, Chater KF, Cerdeno-Tarraga AM, Challis GL, Thompson NR, James KD, Harris DE, Quail MA, Kieser H, Harper D. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature. 2002;417:141–7.

    Article  PubMed  Google Scholar 

  31. Hong K, Gao AH, Xie QY, Gao H, Zhuang L, Lin HP, Yu HP, Li J, Yao XS, Goodfellow M, Ruan JS. Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. Mar Drugs. 2009;7:24–44.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Pandey A, Shukla A, Majumdar SK. Utilization of carbon and nitrogen sources by Streptomyces kanamyceticus M27 for the production of an anti-bacterial antibiotic. Afr J Biotech. 2005;4:909–10.

    CAS  Google Scholar 

  33. Ramesh S, Rajesh M, Mathivanan N. Characterization of a thermostable alkaline protease produced by marine Streptomyces fungicidicus MML1614. Bioprocess Biosyst Eng. 2009;32:791–800.

    Article  CAS  PubMed  Google Scholar 

  34. Singh SP, Thumar JT, Gohel SD and Purohit MK. Molecular diversity and enzymatic potential of salt-tolerent alkaliphilic actinomycetes. In Mendez A, editors. Current research, technology and education topics in applied microbiology and microbial biotechnology; 2010.

  35. Luo HY, Wang YR, Miao LH, Yang PL, Shi PJ, Fang CX, Yao B, Fan YL. Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. Int J Syst Evol Microbiol. 2009;59:863–8.

    Article  CAS  PubMed  Google Scholar 

  36. Vasavada SH, Thumar JT, Singh SP. Secretion of a potent antibiotic by salt- tolerent and alkaliphilic actinomycete Streptomyces sannanensis strain RJT-1. Curr Sci. 2006;91:1393–7.

    CAS  Google Scholar 

  37. Okami Y. Marine microorganisms as a source of bioactive agents. Microb Ecol. 1986;12:65–78.

    Article  CAS  PubMed  Google Scholar 

  38. Bernfield P. Amylases α and β. In: Methods in enzymology. 1st edition. New York, USA: Academic Press; 1955. p. 149–58.

Download references

Acknowledgements

The author’s great fully acknowledge the financial support given by the Ministry of Earth Sciences, Government of India, New Delhi, to conduct the survey and research. The authors are thankful to Dr. M. A. Atmanand, Director, National Institute of Ocean Technology, Chennai for providing necessary facilities to perform this research.

Funding

Not Applicable.

Author information

Authors and Affiliations

  1. Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Dollygunj, Port Blair, 744103, Andaman and Nicobar Islands, India

    Balakrishnan Meena, Lawrance Anburajan & Nambali Valsalan Vinithkumar

  2. Marine Biotechnology Division, Ocean Science and Technology for Islands Group, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Chennai, 600100, Tamil Nadu, India

    Gopal Dharani

  3. Department of Marine Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India

    Munir Ahamed Johnthini

Authors
  1. Balakrishnan Meena
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lawrance Anburajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Munir Ahamed Johnthini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nambali Valsalan Vinithkumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gopal Dharani
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

The research concept and the experiments were executed by BM, LA and MAJ. NVV and GD analyzed the data and reviewed the manuscript. All of the authors approved the final manuscript.

Corresponding authors

Correspondence to Balakrishnan Meena or Lawrance Anburajan.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Ethics approval and consent to participate

Not Applicable.

Consent for publication

Not Applicable.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Meena, B., Anburajan, L., Johnthini, M.A. et al. Exploration of mangrove-associated actinobacteria from South Andaman Islands, India. Syst Microbiol and Biomanuf 3, 702–718 (2023). https://doi.org/10.1007/s43393-022-00134-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s43393-022-00134-3

Keywords

Search

Navigation