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Characterization of a thermostable alkaline protease produced by marine Streptomyces fungicidicus MML1614

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Abstract

Totally 191 different marine actinomycetes were isolated from 256 different marine samples collected from the Bay of Bengal and its associated Pulicat lake and Pichavaram mangrove, India. Among them, 157 produced caseinase, 113 produced gelatinase and 108 produced both the protease enzymes. An isolate coded as MML1614 was selected for further study as it exhibited high proteolytic activity. The MML1614 was identified as Streptomyces fungicidicus based on polyphasic taxonomical approach including 16S rRNA sequence analysis. The culture conditions were standardized for the growth and protease production in S. fungicidicus MML1614. The protease was isolated from a 6-day-old culture filtrate of S. fungicidicus MML1614 and partially purified up to 4.5-fold. The protease was optimally active at pH 9 and 40 °C and it was stable up to pH 11 and 60 °C. PMSF and NaCl inhibited the enzyme activity up to 22 and 11%, respectively. The partially purified protease removed the blood stain more effectively when combined with different detergents than the detergents alone.

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  1. Biocontrol and Microbial Metabolites Lab, Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, 600 025, Tamil Nadu, India

    Subramani Ramesh, Mahalingam Rajesh & Narayanasamy Mathivanan

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  1. Subramani Ramesh
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  2. Mahalingam Rajesh
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  3. Narayanasamy Mathivanan
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Correspondence to Narayanasamy Mathivanan.

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Ramesh, S., Rajesh, M. & Mathivanan, N. Characterization of a thermostable alkaline protease produced by marine Streptomyces fungicidicus MML1614. Bioprocess Biosyst Eng 32, 791–800 (2009). https://doi.org/10.1007/s00449-009-0305-1

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