Skip to main content
SpringerLink
Log in

Alleviation of copper toxicity on chloroplast antioxidant capacity and photosystem II photochemistry of wheat by hydrogen sulfide

  • Short Communication
  • Published:
Brazilian Journal of Botany Aims and scope Submit manuscript

Abstract

This study investigated the effect of H2S on chloroplast antioxidant capacity and chlorophyll fluorescence of wheat seedlings under copper stress. The results showed that copper stress significantly increased the activities of ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD), glutathione peroxidase (GPX), and the contents of MDA and H2O2 in chloroplast and non-photochemical quenching (qN), compared with control. However, copper stress significantly reduced the values of maximum photochemical efficiency of PSII (Fv/Fm), photochemical quenching (qP), quantum efficiency of photochemistry (ΦPSII), and the net photosynthetic rate (Pn), compared with control. Meanwhile, copper stress significantly reduced the fresh and dry weight per plant. Compared with copper stress alone, pretreatment with different concentrations of NaHS plus copper stress enhanced the activities of the above enzymes in chloroplast; and the values of Fv/Fm, qP, qN, ΦPSII, and Pn, especially for copper plus 0.8 mM NaHS. Compared with copper stress alone, pretreatments with different concentrations of NaHS plus copper stress also reduced the contents of MDA and H2O2 in chloroplast and increased the fresh and dry weight per plant, especially for copper plus 0.8 mM NaHS. Our results suggested that H2S has important role for acquisition of copper tolerance by enhancing chloroplast antioxidant capacity and the function of photosynthetic apparatus of wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Alaoui-Sossé B, Genet P, Vinit-Dunand F, Toussaint ML, Epron D, Badot PM (2004) Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant Sci 166:1213–1218

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Brennan T, Frenkel C (1977) Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol 59:411–416

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Calderwood A, Kopriva S (2014) Hydrogen sulfide in plants: from dissipation of excess sulfur to signaling molecule. Nitric Oxide 41:72–78

    Article  CAS  PubMed  Google Scholar 

  • Contreras L, Mella D, Moenne A, Correa JA (2009) Differential responses to copper-induced oxidative stress in the marine macroalgae Lessonia nigrescens and Scytosiphon lomentaria (Phaeophyceae). Aquat Toxicol 94:94–102

    Article  CAS  PubMed  Google Scholar 

  • Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Holzer R, Feller U (2004) Biochemical changes in barley plants after excessive supply of copper and manganese. Environ Exp Bot 52:253–266

    Article  CAS  Google Scholar 

  • Diao M, Ma L, Wang J, Cui J, Fu A, Liu H (2014) Selenium promotes the growth and photosynthesis of tomato seedlings under salt stress by enhancing chloroplast antioxidant defense system. J Plant Growth Regul 33:671–682

    Article  CAS  Google Scholar 

  • Fox T, Guerinot M (1998) Molecular biology of cation transport in plants. Annu Rev Plant Phys 49:669–696

    Article  CAS  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases occurrence in higher plants. Plant Physiol 59:309–314

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 12:909–1026

    Article  Google Scholar 

  • Grace SC, Logan BA (1996) Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. Plant Physiol 112:1631–1640

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hancock JT, Whiteman M (2014) Hydrogen sulfide and cell signaling: team player or referee? Plant Physiol Biochem 78:37–42

    Article  CAS  PubMed  Google Scholar 

  • Hazen MJ, Juarranz A, Stockert JC, Gonzalez A, Beltran-Porter D (1988) Effect of copper coordination complexes on sister-chromatid exchanges in plant cells. Mutat Res 207:135–139

    Article  CAS  PubMed  Google Scholar 

  • He ZQ, Zou ZR, He CX (2006) Effect of AM F on GSH-Px activity and cell membrane osmosis of tomato. J Northwest Sci Tech Univ Agri For (Nat Sci Ed) 34:53–57

    Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198

    Article  CAS  PubMed  Google Scholar 

  • Kumar A, Parsad MNV (2015) Lead-induced toxicity and interference in chlorophyll fluorescence in Talinum triangulare grown hydroponically. Photosynthetica 53:66–71

    Article  CAS  Google Scholar 

  • Li L, Wang Y, Shen W (2012) Roles of hydrogen sulfide and nitric oxide in the alleviation of cadmium-induced oxidative damage in alfalfa seedling roots. Biometals 25:617–631

    Article  CAS  PubMed  Google Scholar 

  • Lisjak M, Teklic T, Wilson ID, Whiteman M, Hancock JT (2013) Hydrogen sulfide: environmental factor or signalling molecule? Plant Cell Environ 36:1607–1616

    Article  CAS  PubMed  Google Scholar 

  • Lombardi L, Sebastiani L (2005) Copper toxicity in Prunus cerasifera: growth and antioxidant enzymes responses of in vitro grown plants. Plant Sci 168:797–802

    Article  CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880

    CAS  Google Scholar 

  • Rastgoo L, Alemzadeh A (2011) Biochemical responses of Gouan (Aeluropus littoralis) to heavy metals stress. Aust J Crop Sci 5:375–383

    CAS  Google Scholar 

  • Shan CJ, Dai HP, Sun YF (2012) Hydrogen sulfide protects wheat seedlings against copper stress by regulating the ascorbate and glutathione metabolism in leaves. Aust J Crop Sci 6:248–254

    CAS  Google Scholar 

  • Vinit-Dunand F, Epron D, Alaoui-Sosse B, Badot PM (2002) Effects of copper on growth and on photosynthesis of mature and expanding leaves in cucumber plants. Plant Sci 163:53–58

    Article  CAS  Google Scholar 

  • Wang H, Feng T, Peng X, Yan M, Tang X (2009) Up-regulation of chloroplastic antioxidant capacity is involved in alleviation of nickel toxicity of Zea mays L. by exogenous salicylic acid. Ecotox Environ Safe 72:1354–1362

    Article  CAS  Google Scholar 

  • Wang L, Hou Z, Hou L, Zhao F, Liu X (2012a) H2S induced by H2O2 mediates drought-induced stomatal closure in Arabidopsis thaliana. Chin Bull Bot 47:217–225

    CAS  Google Scholar 

  • Wang Y, Li L, Cui W, Xu S, Shen W, Wang R (2012b) Hydrogen sulfide enhances alfalfa (Medicago sativa) tolerance against salinity during seed germination by nitric oxide pathway. Plant Soil 351:107–119

    Article  CAS  Google Scholar 

  • Zhang H, Hu LY, Hu KD, He YD, Wang SH, Luo JP (2008) Hydrogen sulfide promotes wheat seed germination and alleviates the oxidative damage against copper stress. J Integr Plant Biol 50:1518–1529

    Article  CAS  PubMed  Google Scholar 

  • Zhang H, Tang J, Liu XP, Wang Y, Yu W, Peng WY, Fang F, Ma DF, Wei ZJ, Hu LY (2009) Hydrogen sulfide promotes root organogenesis in Ipomoea batatas, Salix matsudana and Glycine max. J Integr Plant Biol 51:1086–1094

    Article  CAS  PubMed  Google Scholar 

  • Zhang H, Hu LY, Li P, Hu KD, Jiang CX, Luo JP (2010) Hydrogen sulfide alleviated chromium toxicity in wheat. Biol Plantarum 54:743–747

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was funded by The Starting Fund for High-level Talents (Doctor) of the Henan Institute of Science and Technology (2011010) and the National Natural Science Foundation of China (U1204301).

Author information

Authors and Affiliations

  1. Henan Institute of Science and Technology, Xinxiang, 453003, China

    Haifang Dai & Changjuan Shan

  2. Collaborative Innovation Center of Modern Biological Breeding, Xinxiang, 453003, Henan Province, China

    Haifang Dai & Changjuan Shan

  3. College of Life Sciences, Shangqiu Normal University, Shangqiu, 476000, China

    Yajun Xu & Longfei Zhao

Authors
  1. Haifang Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yajun Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Longfei Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Changjuan Shan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Changjuan Shan.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dai, H., Xu, Y., Zhao, L. et al. Alleviation of copper toxicity on chloroplast antioxidant capacity and photosystem II photochemistry of wheat by hydrogen sulfide. Braz. J. Bot 39, 787–793 (2016). https://doi.org/10.1007/s40415-016-0250-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40415-016-0250-6

Keywords

Search

Navigation