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The influence of phytohormones, wheat ovary co-culture, and temperature stress on anther culture response of watermelon (Citrullus lanatus L.)

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An Erratum to this article was published on 04 September 2015

Abstract

The anther culture response of Citrullus lanatus L. was studied to evaluate the effect of growth regulators and wheat ovary-conditioned medium in Charleston Gray cultivar and the effect of temperature pretreatment in Crimson sweet cultivar. Callus-mediated embryogenesis was induced on Murashige and Skoog (MS) medium supplemented with 2, 4-dichlorophenoxyacetic acid (2,4-D) and a-naphthaleneacetic acid alone as well as 2,4-D in combination with N6-benzylaminopurine/kinetin (BAP/KN). Optimal percentage of embryogenic calli (EC) and mean number of embryos per anther (ME/A) were induced on MS medium supplemented with 2.0 µM 2, 4-D and 1.5 µM BAP in cultivar of Charleston Gray. In this cultivar, the inclusion of 10 wheat ovaries in the induction medium significantly increased the percentage of EC and ME/A up to 2.5- and 3-fold when compared with that in the control, respectively. Low-temperature pretreatment of flower buds (4 °C for 2 and 5 days) and cultured anthers (4 °C for 4 and 8 days) were tested in Crimson sweet cultivar and best response in terms of %EC and ME/A was obtained with pretreatment of cultured anthers at 4 °C for 4 days and flower buds at 4 °C for 2 days. Also, heat pretreatments of the cultured anthers at 30 °C for 7 and 32 °C for 2 days produced the highest percentage of EC compared to control and other heat pretreatments in Crimson sweet cultivar. The highest frequency of ME/A was obtained from cultured anthers incubated at 32 °C for 2 days. Embryos germinated into plantlets on MS medium containing 2.22 µM BAP, 3 % sucrose, and 0.8 % agar. The root tips of 12 regenerated plantlets were analyzed for ploidy level, of which 10 have been haploids.

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Figs. 1–12
Fig. 13
Fig. 14

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Abbreviations

EC:

Embryogenic callus

ME/A:

Mean number of embryo per anther

2, 4-D:

2-Dichlorophenoxyacetic acid

MS:

Murashige and Skoog

NAA:

a-Naphthaleneacetic acid

BAP:

N6-benzylaminopurine

IBA:

Indole-3-butyric acid

KIN:

Kinetin

PAA:

Phenylacetic acid

DH:

Doubled haploid

References

  • Abdollahi MR, Moini A, Javaran MJ (2004) Interactive effects of shock and culture density on embryo induction in isolated microspore culture of Brassica napus L. cv. Global. Iran J Biotechnol 2:97–100

    Google Scholar 

  • Abdollahi M, Ghazanfari P, Corral-Martínez P, Moieni A, Seguí-Simarro J (2012) Enhancing secondary embryogenesis in Brassica napus by selecting hypocotyl-derived embryos and using plant-derived smoke extract in culture medium. Plant Cell Tissue Organ Culture 110:307–315

    Article  Google Scholar 

  • Ashok Kumar HG, Murthy HN (2004) Effect of sugars and amino acids on androgenesis of Cucumis sativus L. Plant Cell Tissue Organ Culture 78:201–208

    Article  Google Scholar 

  • Ashok Kumar HG, Murthy HN, Paek KY (2003) Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L. Sci Hortic 98:213–222

    Article  CAS  Google Scholar 

  • Ashok Kumar HG, Ravishankar BV, Murthy HN (2004) The influence of polyamines on androgenesis of Cucumis sativus L. Eur J Hortic Sci 69:201–205

    Google Scholar 

  • Bhaskaran S, Smith RH (1990) Regeneration in cereal tissue culture: a review. Crop Sci 30:1328–1337

    Article  CAS  Google Scholar 

  • Bobkov S (2014) Obtaining calli and regenerated plants in anther cultures of pea. Czech J Genet Plant Breed 50:123–129

    Google Scholar 

  • Broughton S (2008) Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.). Plant Cell ue Organ Culture 95:185–195

    Article  Google Scholar 

  • Cistué L, Soriano M, Castillo AM, Vallés MP, Sanz JM, Echávarri B (2006) Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture. Plant Cell Rep 25:257–264

    Article  PubMed  Google Scholar 

  • Cistué L, Romagosa I, Batlle F, Echávarri B (2009) Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture. Plant Cell Rep 28:727–735

    Article  PubMed  Google Scholar 

  • Dryanovska OA (1985) Induced callus in vitro from ovaries and anthers of spicies from the Cucurbitaceae family. C R Acad Bulgare Sci 38:1243–1244

    Google Scholar 

  • Ellul PLC, Naval MM, Nogueral FJ, Sanchez S, Atarés A, Moreno V, Corella P, Dirks R (2007) Watermelon biotechnology. Agriculture and forestry, transgenic crops, vol 60. Springer Verlag, Berlin, pp 129–165

    Google Scholar 

  • Eudes F, Amundsen E (2005) Isolated microspore culture of Canadian 6 triticale cultivars. Plant Cell Tissue Organ Culture 82:233–241

    Article  CAS  Google Scholar 

  • Gürsöz N, Abak K, Pitrat M, Rode JC, Dumas de Vaulx R (1991) Obtention of haploid plants induced by irradiated pollen in watermelon (Citrullus lanatus). Rep Cucurbit Genet Coop 11:109–110

    Google Scholar 

  • Hu T, Kasha KJ (1997) Improvement of isolated microspore culture of wheat (Triticum aestivum L) through ovary co-culture. Plant Cell Rep 16:520–525

    Article  CAS  Google Scholar 

  • Kernan Z, Ferrie AMR (2006) Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria). Plant Cell Rep 25:274–280

    Article  CAS  PubMed  Google Scholar 

  • Koleva-Gudeva L, Trajkova F, Dimeska G, Spasenoski M (2009) Androgenesis efficiency in anther culture of pepper (Capsicum annuum L.). Acta Hortic (ISHS) 830:183–190

    Google Scholar 

  • Kozak K, Galek R, Waheed M, Sawicka-Sienkiewicz E (2012) Anther culture of Lupinus angustifolius: callus formation and the development of multicellular and embryo-like structures. Plant Growth Regul 66:145–153

    Article  CAS  Google Scholar 

  • Lantos C, Juhasz AG, Somogyi G, Otvos K, Vagi P, Mihaly R, Kristof Z, Somogyi N, Pauk J (2009) Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co–culture with ovary tissues of pepper or wheat. Plant Cell Tissue Organ Culture 97:285–293

    Article  Google Scholar 

  • Lazarte JE, Sasser CC (1982) Asexual embryogenesis and plantlet development in anther culture of Cucumis sativus L. HortScience 17:88

    Google Scholar 

  • Letarte J, Simion E, Miner M, Kasha K (2006) Arabinogalactans and arabinogalacta-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture. Plant Cell Rep 24:691–698

    Article  CAS  PubMed  Google Scholar 

  • Li H, Devaux P (2001) Enhancement of microspore culture efficiency of recalcitrant barley genotypes. Plant Cell Rep 20:475–481

    Article  CAS  Google Scholar 

  • Lu R, Wang Y, Sun Y, Shan L, Chen P, Huang J (2008) Improvement of isolated microspore culture of barley (Hordeum vulgare L.): the effect of floret co-culture. Plant Cell Tissue Organ Culture 93:21–27

    Article  Google Scholar 

  • Maheshwari SC, Rashid A, Tyagy AK (1982) Haploid from pollen grains-retrospect and prospect. Am J Bot 69:865–879

    Article  Google Scholar 

  • Mejza SJ, Morgant V, DiBona DE, Wong JR (1993) Plant regeneration from isolated microspores of Triticum aestivum. Plant Cell Rep 12:149–153

    Article  CAS  PubMed  Google Scholar 

  • Metwally E, Moustafa SA, El-Sawy BI, Shalaby TA (1998) Haploid plantlets derived by anther culture of Cucurbita pepo. Plant Cell Tissue Org Culture 52:171–176

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Pathirana R, Frew T, Hedderley D, Timmerman-Vaughan G, Morgan ER (2011) Haploid and doubled haploid plants from developing male and female gametes of Gentiana triflora. Plant Cell Rep 30:1055–1065

    Article  CAS  PubMed  Google Scholar 

  • Peng M, Wolyn DJ (1999) Improved callus formation and plant regeneration for shed microspore culture in asparagus (Asparagus officinalis L.). Plant Cell Rep 18:954–958

    Article  CAS  Google Scholar 

  • Perera PIP, Hocher V, Verdeil J-L, Bandupriya HDD, Yakandawala DMD, Weerakoon LK (2008) Androgenic potential in coconut (Cocos nucifera L.). Plant Cell Tissue Organ Culture 92:293–302

    Article  CAS  Google Scholar 

  • Perera PIP, Yakandawala DMD, HocherV Verdeil JL, Weerakoon LK (2009) Effect of growth regulators on microspore embryogenesis in coconut anthers. Plant Cell Tissue Organ Culture 96:171–180

    Article  CAS  Google Scholar 

  • Perera PIP, Ordoñez CA, Becerra Lopez-Lavalle LA, Dedicova B (2014) A milestone in the doubled haploid pathway of cassava. Protoplasma 251:233–246

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Qin X, Rotino GL (1993) Anther culture of several sweet and hotpepper genotypes. Capsicum Eggplant Newsl 12:59–62

    Google Scholar 

  • Rodrigues LR, Oliveira JMS, Mariath JEA, Bodanese-Zanettini MH (2005) Histology of embryogenic responses in soybean anther culture. Plant Cell Tissue Organ Culture 80:129–137

    Article  Google Scholar 

  • Sarı N, Abak K, Pitrat M, Rode JC, de Vaulx RD (1994) Induction of parthenogenetic haploid embryos after pollination by irradiated pollen in watermelon. HortScience 29:1189–1190

    Google Scholar 

  • Sauton A, Dumas de Vaulx R (1987) Production of haploid plants in melon (Cucumis melo L.) as a result of gynogenesis induced by irradiated pollen. Agronomie 7:141–147

    Article  Google Scholar 

  • Sen C, Singh RP, Singh MK, Singh HB (2011) Effect of cold pretreatment on anther culture of Boro rice Hybrids. Int J Plant Reprod Biol 3:69–73

    Google Scholar 

  • Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesiss. Physiol Plant 127:519–534

    Article  CAS  Google Scholar 

  • Sibi M, Dumas de Vaulx R, Chambonnet D (1979) Obtaining haploid plants by in vitro androgenesis in red pepper (Capsicum annuum L.). Ann Amelior Plant 29:583–606

    Google Scholar 

  • Slama-Ayed O, De Buyser J, Picard E, Trifa Y, Amara HS (2010) Effect of pre-treatment on isolated microspores culture ability in durum wheat (Triticum turgidum subsp. Durum Desf.). J Plant Breed Crop Sci 2:30–38

    CAS  Google Scholar 

  • Song H, Lou QF, Luo XD, Wolukau J, Diao WP, Qian CT, Chen JF (2007) Regeneration of doubled haploid plants by androgenesis of cucumber (Cucumis sativus L.). Plant Cell Tissue Organ Culture 90:245–254

    Article  CAS  Google Scholar 

  • Swaminathan MS, Singh MP (1958) X-ray induced somatic haploidy in watermelon. Curr Sci 27:63–64

    Google Scholar 

  • Touraev A, Vicente O, Heberle-Bors E (1997) Initiation of embryogenesis by stress. Trends Plant Sci 2:297–302

    Article  Google Scholar 

  • Xue GR, Yu WY, Fei KW (1983) Watermelon plants derived by in vitro anther culture. Plant Physiol Commun 4:40–42 (In Chinese)

    Google Scholar 

  • Yadollahi A, Abdollahi M, Moieni A, Danaee M (2011) Effects of carbon source, polyethylene glycol and abscisic acid on secondary embryo induction and maturation in rapeseed (Brassica napus L.) microspore-derived embryos. Acta Physiol Plant 33:1905–1912

    Article  CAS  Google Scholar 

  • Zhao FC, Nilanthi D, Yang YS, Wu H (2006) Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.). Plant Cell Tissue Organ Culture 86:55–62

    Article  Google Scholar 

  • Zheng MY, Weng Y, Liu W, Konzak CF (2002) The effect of ovary-conditioned medium on microspore embryogenesis in common wheat (Triticum aestivum L.). Plant Cell Rep 20:802–807

    Article  CAS  Google Scholar 

  • Zheng MY, Liu W, Weng Y, Polle E, Konzak CF (2003) Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Springer, Netherlands, pp 83–94

    Chapter  Google Scholar 

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, P.O.Box 657833131, Hamedan, Iran

    M. R. Abdollahi, M. Darbandi & Y. Hamidvand

  2. Department of Agricultural Biotechnology, University of Kurdistan, Sanandaj, 6617715175, Iran

    M. Majdi

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  1. M. R. Abdollahi
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  2. M. Darbandi
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  3. Y. Hamidvand
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  4. M. Majdi
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Correspondence to M. R. Abdollahi.

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Abdollahi, M.R., Darbandi, M., Hamidvand, Y. et al. The influence of phytohormones, wheat ovary co-culture, and temperature stress on anther culture response of watermelon (Citrullus lanatus L.). Braz. J. Bot 38, 447–456 (2015). https://doi.org/10.1007/s40415-015-0152-z

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