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A Combination of Surgical and Chemical Induction in a Rabbit Model for Osteoarthritis of the Knee

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Appropriate animal models of osteoarthritis (OA) are essential to develop new treatment modalities for OA. A combination of surgical and chemical induction could be appropriate for OA models.

Methods:

Rabbit knee OA models developed by surgical induction (anterior cruciate ligament transection [ACLT]), chemical induction (monosodium iodoacetate [MIA] injection), and a combination of both were compared to assess compositional and structural destruction of the knee joint. Twenty-one New Zealand white rabbits were randomly divided into 3 groups to induce OA (group 1: ACLT, n = 3; group 2: MIA [3, 6, 9 mg] injection, n = 9; group 3: ACLT + MIA [3, 6, 9 mg] injection, n = 9).

Results:

In all groups, the Modified Mankin score was significantly higher in the osteoarthritis-induced knee than in the control. Modified Mankin scores were compared by category. The ACLT group was observed to score high in cartilage structure. In the MIA group, chondrocytes and matrix staining showed higher scores, and the ACLT+MIA group scored higher in all categories for cartilage structure, chondrocytes, matrix staining, and tidemark integrity. The ACLT + 3 mg MIA showed definite OA characteristics such as cartilage surface destruction and degeneration of cartilage layers, and the ACLT + 6 mg MIA and ACLT + 9 mg MIA showed more prominent OA characteristics such as cartilage surface destruction, matrix disorganization, and osteophyte formation.

Conclusion:

The combination of MIA injection and ACLT could be an appropriate method for OA induction in rabbit models.

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Acknowledgements

This study received no funding.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Uijeongbu St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Cheonbo-ro, Uijeongbu-si, Gyeonggi-do, 271, Republic of Korea

    Eun Jeong Go, Seon Ae Kim & Seok Jung Kim

  2. The Rheumatism Research Center, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea

    Mi-La Cho & Kwan Soo Lee

  3. Faculty of Health and Social Care, Institute of Medical Sciences, Canterbury Christ Church University, Kent, UK

    Asode Ananthram Shetty

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  1. Eun Jeong Go
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  2. Seon Ae Kim
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Correspondence to Seok Jung Kim.

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Ethical statement

The animal studies were performed after receiving approval of the Institutional Animal Care and Use Committee (IACUC) (Approval No. UJA2019-10A).

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Go, E.J., Kim, S.A., Cho, ML. et al. A Combination of Surgical and Chemical Induction in a Rabbit Model for Osteoarthritis of the Knee. Tissue Eng Regen Med 19, 1377–1388 (2022). https://doi.org/10.1007/s13770-022-00488-8

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  • DOI: https://doi.org/10.1007/s13770-022-00488-8

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