Abstract
Several studies have shown that the plant 14-3-3 gene family plays an important role in plant growth and development, as well as its response to abiotic and biotic stresses. At present, the 14-3-3 gene family has been found successively in many plants; however, no detailed characterization and expression profiles of the 14-3-3 gene family are found in melon. Thus, the author performed genome-wide identification, characterization and expression pattern in different tissues and under various abiotic and biotic stresses by analyzing the published RNA-Seq raw data from NCBI database. Nine 14-3-3 genes in melon were identified and classified as non-epsilon and epsilon groups based on the orthologs of Arabidopsis 14-3-3 genes and dispersedly distributed on five chromosomes. The majority of melon 14-3-3 members contained 300 amino acids or less, which might localize in the nucleus. Members of the epsilon group commonly contained more exons or introns than those of the non-epsilon group. Gene duplication analysis indicated that segmental duplication was dominant in the expansion of the melon 14-3-3 family. Many cis-elements in response to different stresses and hormones were predicted by using the PlantCARE server. The published RNA-Seq showed that several 14-3-3 genes showed significant expression changes in different tissues, during fruit development and under salt, cold and pathogen stresses at a certain time point. These results provided a general introduction to melon 14-3-3 genes and a preliminary basis for later functional verification.
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Acknowledgements
The author thanks Mr. Jiannong Zhang in Gansu Agricultural University for his providing melon inbreeding lines. The author also thanks Ms. Lilan Chen in Gansu Agricultural University for her help with manuscript revision.
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This work was supported by Research Program Sponsored by State Key Laboratory of Aridland Crop Science, Gansu Agricultural University (GSCS-2020-09), China.
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GYZ and BQW designed, conceived and performed the experiments. GYZ carried out the bioinformatics, edited the data, figures and tables, draft the manuscript. All authors read and approved the final manuscript.
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Zhang, G., Wei, B. Characterization of 14-3-3 gene family and their expression patterns under abiotic and biotic stresses in melon (Cucumis melo L.). Hortic. Environ. Biotechnol. 64, 1039–1054 (2023). https://doi.org/10.1007/s13580-023-00532-z
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DOI: https://doi.org/10.1007/s13580-023-00532-z